Corynebacterium aquilae DSM 44791
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2002 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L7CE29|A0A1L7CE29_9CORY ABC transporter substrate-binding protein OS=Corynebacterium aquilae DSM 44791 OX=1431546 GN=CAQU_02480 PE=4 SV=1
MM1 pKa = 7.21 NAEE4 pKa = 3.89 KK5 pKa = 9.91 TLPRR9 pKa = 11.84 VIRR12 pKa = 11.84 RR13 pKa = 11.84 ALAVGACTAPIVLVGCSNGGQSGPSTTVVTVTRR46 pKa = 11.84 SSQSSTPSTSAAAPNSSAVAEE67 pKa = 4.39 PKK69 pKa = 10.3 KK70 pKa = 10.06 DD71 pKa = 3.28 THH73 pKa = 6.01 SQNGGTDD80 pKa = 3.09 GNTFVPEE87 pKa = 4.17 HH88 pKa = 6.01 FPVRR92 pKa = 11.84 NDD94 pKa = 4.12 LIEE97 pKa = 4.74 GPPEE101 pKa = 3.97 LQGKK105 pKa = 7.15 TIVSCLGAEE114 pKa = 4.63 HH115 pKa = 7.41 PGQYY119 pKa = 10.71 DD120 pKa = 3.21 PGLTLLDD127 pKa = 4.42 DD128 pKa = 4.37 GTRR131 pKa = 11.84 VWTAQCQMAYY141 pKa = 10.48 DD142 pKa = 3.89 YY143 pKa = 11.5 GRR145 pKa = 11.84 ADD147 pKa = 3.05 IAGTDD152 pKa = 4.34 DD153 pKa = 3.68 YY154 pKa = 11.85 SEE156 pKa = 4.64 SDD158 pKa = 2.9 SDD160 pKa = 5.0 YY161 pKa = 11.41 SDD163 pKa = 4.03 TYY165 pKa = 11.85 DD166 pKa = 4.29 DD167 pKa = 5.41 GYY169 pKa = 11.51 SDD171 pKa = 5.01 GYY173 pKa = 10.73 DD174 pKa = 3.21 AAVEE178 pKa = 4.28 EE179 pKa = 4.57 YY180 pKa = 10.92 EE181 pKa = 4.51 EE182 pKa = 4.32 ALEE185 pKa = 4.57 DD186 pKa = 3.97 ALSEE190 pKa = 4.98 LEE192 pKa = 4.52 DD193 pKa = 3.56 NN194 pKa = 4.67
Molecular weight: 20.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.973
IPC_protein 3.948
Toseland 3.745
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.859
Rodwell 3.783
Grimsley 3.656
Solomon 3.935
Lehninger 3.884
Nozaki 4.05
DTASelect 4.266
Thurlkill 3.795
EMBOSS 3.872
Sillero 4.075
Patrickios 1.163
IPC_peptide 3.923
IPC2_peptide 4.05
IPC2.peptide.svr19 3.947
Protein with the highest isoelectric point:
>tr|A0A1L7CD50|A0A1L7CD50_9CORY Uncharacterized protein OS=Corynebacterium aquilae DSM 44791 OX=1431546 GN=CAQU_00335 PE=3 SV=1
MM1 pKa = 7.28 GVVIYY6 pKa = 10.52 GSFGQLWWEE15 pKa = 3.59 KK16 pKa = 9.66 RR17 pKa = 11.84 VRR19 pKa = 11.84 VFVWRR24 pKa = 11.84 CAPVFYY30 pKa = 11.04 ALFTLVKK37 pKa = 10.24 IPVTSGWKK45 pKa = 9.78 SMANIFLAQPCAWRR59 pKa = 11.84 VVSLQVLGCGKK70 pKa = 8.36 TSYY73 pKa = 10.68 RR74 pKa = 11.84 KK75 pKa = 8.56 GVVMAGHH82 pKa = 7.16 RR83 pKa = 11.84 EE84 pKa = 3.88 PVSWRR89 pKa = 11.84 EE90 pKa = 3.74 QLRR93 pKa = 11.84 ASLKK97 pKa = 8.93 QTADD101 pKa = 3.27 PDD103 pKa = 3.33 ATIRR107 pKa = 11.84 FGVGMGVLFFVLNSFRR123 pKa = 11.84 DD124 pKa = 3.78 EE125 pKa = 4.2 VGLVLNLGLSLSLVAVFGIYY145 pKa = 10.45 GLAARR150 pKa = 11.84 NPGGRR155 pKa = 11.84 PRR157 pKa = 11.84 YY158 pKa = 9.15 VRR160 pKa = 11.84 GVVAAAVLIGVLAAALALVGQFFF183 pKa = 3.95
Molecular weight: 20.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.838
IPC_protein 10.73
Toseland 10.672
ProMoST 10.452
Dawson 10.804
Bjellqvist 10.555
Wikipedia 11.038
Rodwell 10.965
Grimsley 10.862
Solomon 10.921
Lehninger 10.877
Nozaki 10.687
DTASelect 10.54
Thurlkill 10.701
EMBOSS 11.082
Sillero 10.73
Patrickios 10.701
IPC_peptide 10.921
IPC2_peptide 9.736
IPC2.peptide.svr19 8.356
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2002
0
2002
655671
40
4411
327.5
35.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.056 ± 0.076
0.868 ± 0.017
6.186 ± 0.047
5.839 ± 0.053
3.297 ± 0.032
8.265 ± 0.049
2.335 ± 0.036
5.106 ± 0.041
3.457 ± 0.052
9.28 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.255 ± 0.024
2.964 ± 0.042
5.229 ± 0.045
3.452 ± 0.031
5.776 ± 0.061
5.436 ± 0.035
6.473 ± 0.068
8.229 ± 0.058
1.279 ± 0.02
2.219 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here