Drosophila immigrans Nora virus

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075E230|A0A075E230_9VIRU VP1 OS=Drosophila immigrans Nora virus OX=1500866 GN=VP1 PE=4 SV=1
MM1 pKa = 7.65ALKK4 pKa = 10.46QEE6 pKa = 4.1IFEE9 pKa = 4.34QNSTLFEE16 pKa = 4.1VLDD19 pKa = 4.11EE20 pKa = 4.47NEE22 pKa = 3.8VMEE25 pKa = 4.51IKK27 pKa = 10.3EE28 pKa = 4.12IQSSVTAVQTQVDD41 pKa = 4.02QQKK44 pKa = 10.33LQLDD48 pKa = 4.17GLARR52 pKa = 11.84RR53 pKa = 11.84VDD55 pKa = 3.92LNQARR60 pKa = 11.84NEE62 pKa = 4.02EE63 pKa = 4.13QFVNFNTMLIATNTEE78 pKa = 3.38VDD80 pKa = 3.5KK81 pKa = 11.63LKK83 pKa = 8.78TTTTTLSVSVNNLEE97 pKa = 4.06RR98 pKa = 11.84SVGEE102 pKa = 4.62LEE104 pKa = 4.22TTTQSNFDD112 pKa = 3.8TIFRR116 pKa = 11.84RR117 pKa = 11.84LAITEE122 pKa = 4.19EE123 pKa = 4.19TSSQNKK129 pKa = 9.92SEE131 pKa = 4.09IVTILDD137 pKa = 4.13KK138 pKa = 10.85IDD140 pKa = 4.31DD141 pKa = 4.41LEE143 pKa = 5.5HH144 pKa = 7.26SVEE147 pKa = 4.21SANDD151 pKa = 3.63EE152 pKa = 4.16IAEE155 pKa = 4.22LTDD158 pKa = 3.84SVEE161 pKa = 4.47DD162 pKa = 3.61NQTAITTLSGRR173 pKa = 11.84VTTLADD179 pKa = 3.11RR180 pKa = 11.84VTKK183 pKa = 10.78LEE185 pKa = 4.7NIVQSISPYY194 pKa = 8.79IQRR197 pKa = 11.84QTFYY201 pKa = 10.87RR202 pKa = 11.84IVYY205 pKa = 8.38YY206 pKa = 9.05KK207 pKa = 10.69TNGSMRR213 pKa = 11.84NVGFWANGYY222 pKa = 8.78VRR224 pKa = 11.84YY225 pKa = 10.65SMSFQVGTSATANFFTNLTITSVISSYY252 pKa = 11.14DD253 pKa = 3.35YY254 pKa = 10.86STIPVLKK261 pKa = 9.73GVPVRR266 pKa = 11.84LTGSYY271 pKa = 7.75PTGSNTFEE279 pKa = 4.24SVSGSTAIIYY289 pKa = 9.86DD290 pKa = 3.85DD291 pKa = 3.67NALVV295 pKa = 3.35

Molecular weight: 32.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075E230|A0A075E230_9VIRU VP1 OS=Drosophila immigrans Nora virus OX=1500866 GN=VP1 PE=4 SV=1
MM1 pKa = 7.04NQDD4 pKa = 3.19EE5 pKa = 4.48QKK7 pKa = 11.16KK8 pKa = 10.37KK9 pKa = 9.61GAQLKK14 pKa = 9.54RR15 pKa = 11.84VHH17 pKa = 5.76TVGTQVVDD25 pKa = 3.17TCNRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84QKK33 pKa = 8.27GTKK36 pKa = 8.6MEE38 pKa = 4.3VNFTVKK44 pKa = 10.27RR45 pKa = 11.84NNADD49 pKa = 3.42EE50 pKa = 4.12EE51 pKa = 4.56QKK53 pKa = 10.93FFVTEE58 pKa = 4.06RR59 pKa = 11.84VDD61 pKa = 3.95HH62 pKa = 6.46KK63 pKa = 11.24LEE65 pKa = 4.16KK66 pKa = 9.74QVKK69 pKa = 9.42AEE71 pKa = 3.91AKK73 pKa = 9.81QNHH76 pKa = 5.64TFIKK80 pKa = 10.11PKK82 pKa = 10.7LNFVDD87 pKa = 3.72KK88 pKa = 11.7VEE90 pKa = 4.06VEE92 pKa = 4.31KK93 pKa = 11.52VKK95 pKa = 9.65VTSRR99 pKa = 11.84GKK101 pKa = 9.87EE102 pKa = 3.4RR103 pKa = 11.84AANFAFFKK111 pKa = 10.86EE112 pKa = 4.29LVNSNKK118 pKa = 9.12VEE120 pKa = 4.18QKK122 pKa = 10.4WNVEE126 pKa = 4.01TEE128 pKa = 4.06KK129 pKa = 11.14EE130 pKa = 3.75IDD132 pKa = 3.66EE133 pKa = 4.31VDD135 pKa = 3.57LFFMKK140 pKa = 10.47KK141 pKa = 7.05KK142 pKa = 8.73TKK144 pKa = 9.54PFSGFSIKK152 pKa = 10.71SLGDD156 pKa = 3.52SLKK159 pKa = 10.76VMPDD163 pKa = 3.19EE164 pKa = 4.59KK165 pKa = 10.79HH166 pKa = 6.48ISPQTAMSSINEE178 pKa = 4.08NVATDD183 pKa = 3.59AVVDD187 pKa = 5.13VIALTAQLNQLIKK200 pKa = 10.44RR201 pKa = 11.84VEE203 pKa = 3.87ALEE206 pKa = 4.57LDD208 pKa = 3.68NKK210 pKa = 10.37QLVEE214 pKa = 4.23MNNQLRR220 pKa = 11.84QDD222 pKa = 3.58NEE224 pKa = 3.93KK225 pKa = 10.63LVNEE229 pKa = 5.72GITSIKK235 pKa = 10.03NDD237 pKa = 3.19KK238 pKa = 10.42KK239 pKa = 10.61EE240 pKa = 4.28KK241 pKa = 10.37KK242 pKa = 9.86NSEE245 pKa = 4.1NKK247 pKa = 9.75IEE249 pKa = 3.97QAISKK254 pKa = 7.45TASCMFKK261 pKa = 10.14IVAEE265 pKa = 4.5KK266 pKa = 10.73KK267 pKa = 10.0RR268 pKa = 11.84VTQVTPIVGEE278 pKa = 4.17EE279 pKa = 3.87QCLEE283 pKa = 4.02LQSTIKK289 pKa = 10.32RR290 pKa = 11.84KK291 pKa = 10.2YY292 pKa = 9.19KK293 pKa = 10.23EE294 pKa = 4.13KK295 pKa = 10.32PKK297 pKa = 10.73LPSVPSKK304 pKa = 10.8KK305 pKa = 9.54EE306 pKa = 3.68KK307 pKa = 10.6KK308 pKa = 10.26SIQQTKK314 pKa = 8.31IRR316 pKa = 11.84QWYY319 pKa = 8.62NFNKK323 pKa = 8.19QTIEE327 pKa = 3.88EE328 pKa = 4.23HH329 pKa = 5.39QKK331 pKa = 10.41EE332 pKa = 4.55VLSSVQTDD340 pKa = 3.18VTFAEE345 pKa = 4.59KK346 pKa = 10.6VKK348 pKa = 10.9EE349 pKa = 3.88NGIPKK354 pKa = 9.89QKK356 pKa = 10.41LRR358 pKa = 11.84YY359 pKa = 6.93TVSTTVEE366 pKa = 4.08KK367 pKa = 10.69QPKK370 pKa = 9.66KK371 pKa = 10.43SIHH374 pKa = 6.22HH375 pKa = 6.04YY376 pKa = 8.86GYY378 pKa = 10.84KK379 pKa = 9.72PAGVPNKK386 pKa = 9.75IWWKK390 pKa = 9.09WVTTGTAAEE399 pKa = 4.56AYY401 pKa = 9.42KK402 pKa = 10.48KK403 pKa = 10.76AQTFLFKK410 pKa = 10.08MFKK413 pKa = 10.32RR414 pKa = 11.84EE415 pKa = 4.07MIVFRR420 pKa = 11.84QKK422 pKa = 10.0WICHH426 pKa = 4.84TKK428 pKa = 10.22EE429 pKa = 3.7FNPYY433 pKa = 9.49LSEE436 pKa = 4.24PKK438 pKa = 9.76MVWLEE443 pKa = 3.89NTNEE447 pKa = 3.74YY448 pKa = 10.2PYY450 pKa = 11.08DD451 pKa = 3.8VNVLFEE457 pKa = 5.13FIKK460 pKa = 9.63KK461 pKa = 8.36WRR463 pKa = 11.84MLVTSYY469 pKa = 11.35KK470 pKa = 9.44PGKK473 pKa = 9.4PITNDD478 pKa = 2.5WYY480 pKa = 11.13KK481 pKa = 11.26NKK483 pKa = 10.17QNN485 pKa = 3.82

Molecular weight: 56.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3761

295

2043

940.3

106.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.275 ± 0.634

1.09 ± 0.264

4.892 ± 0.395

7.392 ± 0.431

4.201 ± 0.15

5.238 ± 0.677

1.462 ± 0.216

6.7 ± 0.442

7.471 ± 1.956

8.349 ± 0.85

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.994 ± 0.071

5.477 ± 0.48

4.121 ± 1.139

4.866 ± 0.611

4.307 ± 0.175

6.036 ± 0.659

7.312 ± 0.98

7.844 ± 0.522

1.462 ± 0.253

3.51 ± 0.456
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski