Posidoniimonas polymericola
Average proteome isoelectric point is 5.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4870 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C5YKX3|A0A5C5YKX3_9BACT Putative acetyltransferase OS=Posidoniimonas polymericola OX=2528002 GN=Pla123a_29780 PE=4 SV=1
MM1 pKa = 7.38 CRR3 pKa = 11.84 SIAAITLATLLASQGVCHH21 pKa = 6.06 AVVVASLEE29 pKa = 4.49 TNVAPAGTAGDD40 pKa = 4.24 PYY42 pKa = 11.34 NPTFTAGGPSTTDD55 pKa = 3.41 LLEE58 pKa = 4.54 GLLPTVAIGNLTQEE72 pKa = 4.19 NSLGASALTNGTVATAYY89 pKa = 10.48 GSGDD93 pKa = 3.52 ANSAHH98 pKa = 6.99 IAYY101 pKa = 7.55 ATGGPGEE108 pKa = 4.36 EE109 pKa = 3.66 VTYY112 pKa = 10.97 ALGGIYY118 pKa = 10.35 DD119 pKa = 4.69 LDD121 pKa = 4.45 SIVIFGGWNDD131 pKa = 3.68 AGRR134 pKa = 11.84 DD135 pKa = 3.64 AQSYY139 pKa = 10.07 DD140 pKa = 4.05 LYY142 pKa = 11.04 VSSDD146 pKa = 3.07 GAQNFSLLTNYY157 pKa = 10.61 NNGNGEE163 pKa = 4.1 EE164 pKa = 4.17 QGVVTTPVSHH174 pKa = 6.41 RR175 pKa = 11.84 VEE177 pKa = 4.25 FTEE180 pKa = 5.88 DD181 pKa = 3.35 SLPNLATGVTHH192 pKa = 6.63 VKK194 pKa = 10.32 VDD196 pKa = 4.0 FLDD199 pKa = 3.66 VEE201 pKa = 4.28 NGYY204 pKa = 8.04 TGYY207 pKa = 9.28 TEE209 pKa = 4.28 IDD211 pKa = 3.56 VFGSLVNVPGDD222 pKa = 3.54 ADD224 pKa = 3.75 GDD226 pKa = 4.13 GDD228 pKa = 4.3 VDD230 pKa = 4.69 LDD232 pKa = 4.02 DD233 pKa = 5.52 FFVISNHH240 pKa = 5.89 FFTTPSAIGLDD251 pKa = 3.74 GDD253 pKa = 4.12 VEE255 pKa = 4.27 PDD257 pKa = 3.54 NIVDD261 pKa = 3.79 EE262 pKa = 4.62 KK263 pKa = 11.4 DD264 pKa = 3.1 FRR266 pKa = 11.84 LWKK269 pKa = 10.01 SVASASVLAEE279 pKa = 4.21 FAALGVPEE287 pKa = 5.15 PSTLALIGVSMGLLAGRR304 pKa = 11.84 RR305 pKa = 11.84 RR306 pKa = 11.84 GG307 pKa = 3.38
Molecular weight: 31.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.821
IPC_protein 3.834
Toseland 3.617
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.656
Grimsley 3.516
Solomon 3.821
Lehninger 3.77
Nozaki 3.935
DTASelect 4.177
Thurlkill 3.668
EMBOSS 3.77
Sillero 3.948
Patrickios 1.189
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.842
Protein with the highest isoelectric point:
>tr|A0A5C5YSW6|A0A5C5YSW6_9BACT Quinolinate synthase A OS=Posidoniimonas polymericola OX=2528002 GN=nadA PE=3 SV=1
MM1 pKa = 7.07 NRR3 pKa = 11.84 KK4 pKa = 8.76 NLVVAAVLASAFALGMLTSKK24 pKa = 10.28 QASAGKK30 pKa = 9.95 YY31 pKa = 9.49 GPAGLGANLHH41 pKa = 6.26 RR42 pKa = 11.84 RR43 pKa = 11.84 HH44 pKa = 6.14 VMKK47 pKa = 10.59 QATKK51 pKa = 9.23 NARR54 pKa = 11.84 RR55 pKa = 11.84 GLPVRR60 pKa = 11.84 KK61 pKa = 9.33 SAIVWLSAPQSNLRR75 pKa = 11.84 WARR78 pKa = 3.38
Molecular weight: 8.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.362
IPC2_protein 11.008
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.281
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.019
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4870
0
4870
1793801
30
6968
368.3
40.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.531 ± 0.041
1.092 ± 0.014
6.292 ± 0.029
6.16 ± 0.03
3.367 ± 0.019
8.628 ± 0.043
2.044 ± 0.017
4.036 ± 0.022
2.84 ± 0.028
10.031 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.987 ± 0.015
2.892 ± 0.023
5.582 ± 0.032
3.794 ± 0.023
6.746 ± 0.038
6.052 ± 0.029
5.352 ± 0.029
7.519 ± 0.026
1.538 ± 0.014
2.519 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here