Gordonia phage Benczkowski14
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142KCF1|A0A142KCF1_9CAUD Uncharacterized protein OS=Gordonia phage Benczkowski14 OX=1821550 GN=74 PE=4 SV=1
MM1 pKa = 7.77 IKK3 pKa = 10.33 RR4 pKa = 11.84 IALGLAAGALAFAAPAIAVASPTPAPAYY32 pKa = 10.55 VSTACTISTPTGPAPCPPPIISTNGDD58 pKa = 3.76 NIGGGANSEE67 pKa = 4.47 PINMGDD73 pKa = 4.13 LPSNGPDD80 pKa = 3.25 YY81 pKa = 10.62 TYY83 pKa = 11.03 EE84 pKa = 4.18 RR85 pKa = 11.84 PTYY88 pKa = 10.42 FDD90 pKa = 4.21 DD91 pKa = 5.25 PEE93 pKa = 6.11 PEE95 pKa = 4.13 APAEE99 pKa = 4.1 PEE101 pKa = 4.19 APAEE105 pKa = 4.16 DD106 pKa = 3.71
Molecular weight: 10.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.841
IPC2_protein 3.745
IPC_protein 3.63
Toseland 3.452
ProMoST 3.706
Dawson 3.617
Bjellqvist 3.872
Wikipedia 3.567
Rodwell 3.478
Grimsley 3.376
Solomon 3.579
Lehninger 3.541
Nozaki 3.77
DTASelect 3.897
Thurlkill 3.516
EMBOSS 3.567
Sillero 3.745
Patrickios 0.299
IPC_peptide 3.579
IPC2_peptide 3.719
IPC2.peptide.svr19 3.808
Protein with the highest isoelectric point:
>tr|A0A142KCH7|A0A142KCH7_9CAUD Uncharacterized protein OS=Gordonia phage Benczkowski14 OX=1821550 GN=100 PE=4 SV=1
MM1 pKa = 7.55 GRR3 pKa = 11.84 PLPMASAPRR12 pKa = 11.84 TVTVTKK18 pKa = 10.39 RR19 pKa = 11.84 VRR21 pKa = 11.84 ATAKK25 pKa = 10.07 RR26 pKa = 11.84 GTGINTPLTPLNTVIAMRR44 pKa = 11.84 EE45 pKa = 3.84 RR46 pKa = 11.84 TEE48 pKa = 3.98 ADD50 pKa = 4.0 CVNKK54 pKa = 10.65 DD55 pKa = 2.69 NWAGKK60 pKa = 10.34 DD61 pKa = 3.15 EE62 pKa = 4.18 TRR64 pKa = 11.84 PDD66 pKa = 3.16 EE67 pKa = 4.18 TTAYY71 pKa = 8.42 SWNMTGIQANVKK83 pKa = 9.34 TMVEE87 pKa = 4.21 NQTMRR92 pKa = 11.84 PVQPKK97 pKa = 10.08 NPAPKK102 pKa = 8.74 TARR105 pKa = 11.84 VKK107 pKa = 10.57 KK108 pKa = 9.74 NKK110 pKa = 9.93 SSTRR114 pKa = 11.84 RR115 pKa = 11.84 VDD117 pKa = 3.9 RR118 pKa = 11.84 DD119 pKa = 3.68 DD120 pKa = 4.01 LHH122 pKa = 6.95 FSAVVRR128 pKa = 11.84 YY129 pKa = 9.59 SEE131 pKa = 4.34 VVKK134 pKa = 10.75 RR135 pKa = 11.84 SLWPHH140 pKa = 4.79 RR141 pKa = 11.84 TLRR144 pKa = 11.84 LDD146 pKa = 3.12 VPKK149 pKa = 10.39 RR150 pKa = 11.84 YY151 pKa = 9.58 RR152 pKa = 11.84 ADD154 pKa = 3.34 VAKK157 pKa = 10.84 LCGLL161 pKa = 3.9
Molecular weight: 18.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.692
IPC_protein 10.438
Toseland 10.921
ProMoST 10.599
Dawson 10.979
Bjellqvist 10.672
Wikipedia 11.184
Rodwell 11.228
Grimsley 11.008
Solomon 11.125
Lehninger 11.096
Nozaki 10.891
DTASelect 10.672
Thurlkill 10.891
EMBOSS 11.316
Sillero 10.906
Patrickios 10.95
IPC_peptide 11.14
IPC2_peptide 9.575
IPC2.peptide.svr19 8.722
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
99
0
99
23715
37
3027
239.5
26.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.256 ± 0.656
0.974 ± 0.159
6.384 ± 0.315
6.903 ± 0.367
3.504 ± 0.112
7.641 ± 0.317
1.957 ± 0.176
5.385 ± 0.197
5.313 ± 0.227
7.421 ± 0.258
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.8 ± 0.203
3.981 ± 0.14
5.461 ± 0.333
3.795 ± 0.166
5.676 ± 0.376
5.832 ± 0.183
6.114 ± 0.218
6.793 ± 0.211
1.75 ± 0.089
3.061 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here