Gordonia phage Benczkowski14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Demosthenesvirus; Gordonia virus Kvothe

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A142KCF1|A0A142KCF1_9CAUD Uncharacterized protein OS=Gordonia phage Benczkowski14 OX=1821550 GN=74 PE=4 SV=1
MM1 pKa = 7.77IKK3 pKa = 10.33RR4 pKa = 11.84IALGLAAGALAFAAPAIAVASPTPAPAYY32 pKa = 10.55VSTACTISTPTGPAPCPPPIISTNGDD58 pKa = 3.76NIGGGANSEE67 pKa = 4.47PINMGDD73 pKa = 4.13LPSNGPDD80 pKa = 3.25YY81 pKa = 10.62TYY83 pKa = 11.03EE84 pKa = 4.18RR85 pKa = 11.84PTYY88 pKa = 10.42FDD90 pKa = 4.21DD91 pKa = 5.25PEE93 pKa = 6.11PEE95 pKa = 4.13APAEE99 pKa = 4.1PEE101 pKa = 4.19APAEE105 pKa = 4.16DD106 pKa = 3.71

Molecular weight:
10.64 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A142KCH7|A0A142KCH7_9CAUD Uncharacterized protein OS=Gordonia phage Benczkowski14 OX=1821550 GN=100 PE=4 SV=1
MM1 pKa = 7.55GRR3 pKa = 11.84PLPMASAPRR12 pKa = 11.84TVTVTKK18 pKa = 10.39RR19 pKa = 11.84VRR21 pKa = 11.84ATAKK25 pKa = 10.07RR26 pKa = 11.84GTGINTPLTPLNTVIAMRR44 pKa = 11.84EE45 pKa = 3.84RR46 pKa = 11.84TEE48 pKa = 3.98ADD50 pKa = 4.0CVNKK54 pKa = 10.65DD55 pKa = 2.69NWAGKK60 pKa = 10.34DD61 pKa = 3.15EE62 pKa = 4.18TRR64 pKa = 11.84PDD66 pKa = 3.16EE67 pKa = 4.18TTAYY71 pKa = 8.42SWNMTGIQANVKK83 pKa = 9.34TMVEE87 pKa = 4.21NQTMRR92 pKa = 11.84PVQPKK97 pKa = 10.08NPAPKK102 pKa = 8.74TARR105 pKa = 11.84VKK107 pKa = 10.57KK108 pKa = 9.74NKK110 pKa = 9.93SSTRR114 pKa = 11.84RR115 pKa = 11.84VDD117 pKa = 3.9RR118 pKa = 11.84DD119 pKa = 3.68DD120 pKa = 4.01LHH122 pKa = 6.95FSAVVRR128 pKa = 11.84YY129 pKa = 9.59SEE131 pKa = 4.34VVKK134 pKa = 10.75RR135 pKa = 11.84SLWPHH140 pKa = 4.79RR141 pKa = 11.84TLRR144 pKa = 11.84LDD146 pKa = 3.12VPKK149 pKa = 10.39RR150 pKa = 11.84YY151 pKa = 9.58RR152 pKa = 11.84ADD154 pKa = 3.34VAKK157 pKa = 10.84LCGLL161 pKa = 3.9

Molecular weight:
18.21 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

99

0

99

23715

37

3027

239.5

26.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.256 ± 0.656

0.974 ± 0.159

6.384 ± 0.315

6.903 ± 0.367

3.504 ± 0.112

7.641 ± 0.317

1.957 ± 0.176

5.385 ± 0.197

5.313 ± 0.227

7.421 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.8 ± 0.203

3.981 ± 0.14

5.461 ± 0.333

3.795 ± 0.166

5.676 ± 0.376

5.832 ± 0.183

6.114 ± 0.218

6.793 ± 0.211

1.75 ± 0.089

3.061 ± 0.181

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski