Rosa chinensis (China rose)
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 45026 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P6S819|A0A2P6S819_ROSCH Putative endothelin-converting enzyme 1 OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0318081 PE=4 SV=1
MM1 pKa = 7.81 ANGQPPVDD9 pKa = 3.54 IKK11 pKa = 10.99 KK12 pKa = 9.48 QASSEE17 pKa = 3.82 ICNICWEE24 pKa = 4.15 EE25 pKa = 3.85 LQEE28 pKa = 4.14 VNHH31 pKa = 5.9 VKK33 pKa = 10.38 EE34 pKa = 4.15 MPKK37 pKa = 9.63 CKK39 pKa = 10.18 HH40 pKa = 5.8 MFHH43 pKa = 6.07 VHH45 pKa = 7.41 CINAWLAKK53 pKa = 9.77 NACCPLCRR61 pKa = 11.84 RR62 pKa = 11.84 FAVVPDD68 pKa = 4.74 LISILVQEE76 pKa = 4.9 YY77 pKa = 10.81 EE78 pKa = 5.09 DD79 pKa = 4.93 DD80 pKa = 4.24 HH81 pKa = 9.11 DD82 pKa = 6.09 DD83 pKa = 4.08 DD84 pKa = 5.23 GPYY87 pKa = 10.89 VSIHH91 pKa = 6.87 DD92 pKa = 5.07 LLMEE96 pKa = 5.88 DD97 pKa = 5.51 DD98 pKa = 6.02 DD99 pKa = 4.42 EE100 pKa = 5.5 TDD102 pKa = 4.46 DD103 pKa = 5.5 IIPILIPDD111 pKa = 4.29 HH112 pKa = 6.82 EE113 pKa = 4.93 NGVDD117 pKa = 4.22 PIGLGNVDD125 pKa = 3.41 NAEE128 pKa = 4.16 DD129 pKa = 3.75 VLVGLVEE136 pKa = 3.78 EE137 pKa = 5.38 HH138 pKa = 6.44 IRR140 pKa = 11.84 DD141 pKa = 4.01 YY142 pKa = 11.33 IPILVTYY149 pKa = 9.96 HH150 pKa = 7.04 HH151 pKa = 7.93 DD152 pKa = 4.5 DD153 pKa = 3.82 GLDD156 pKa = 3.55 PMSLGNDD163 pKa = 3.56 DD164 pKa = 5.02 DD165 pKa = 5.9 FDD167 pKa = 3.85 MGLDD171 pKa = 3.71 QEE173 pKa = 4.09 EE174 pKa = 4.14 DD175 pKa = 3.61 AYY177 pKa = 10.23 IEE179 pKa = 4.3 SLVFSSSDD187 pKa = 3.55 DD188 pKa = 3.24 DD189 pKa = 4.64 WYY191 pKa = 11.26 GQDD194 pKa = 4.1 DD195 pKa = 4.57 TEE197 pKa = 4.6 TQNSQQ202 pKa = 2.69
Molecular weight: 22.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.808
IPC_protein 3.834
Toseland 3.617
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.668
Grimsley 3.528
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.202
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.961
Patrickios 0.82
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A2P6PQ00|A0A2P6PQ00_ROSCH Putative transcription factor MYB-HB-like family OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr6g0267581 PE=4 SV=1
MM1 pKa = 7.76 ASLKK5 pKa = 10.47 ASRR8 pKa = 11.84 AAATKK13 pKa = 9.95 SAPSSSRR20 pKa = 11.84 TVPPPLAPARR30 pKa = 11.84 SRR32 pKa = 11.84 PAPPPPSATARR43 pKa = 11.84 SRR45 pKa = 11.84 PAPPPPSATARR56 pKa = 11.84 SRR58 pKa = 11.84 PATSGTASSRR68 pKa = 11.84 HH69 pKa = 4.74 VRR71 pKa = 11.84 TSQASSNKK79 pKa = 9.19 AANPTRR85 pKa = 11.84 ASQRR89 pKa = 11.84 IRR91 pKa = 11.84 QNSGKK96 pKa = 10.69 GGGKK100 pKa = 9.75
Molecular weight: 10.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.735
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.457
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.237
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
45026
0
45026
14462663
40
5463
321.2
35.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.407 ± 0.012
2.021 ± 0.006
5.149 ± 0.009
6.242 ± 0.014
4.418 ± 0.009
6.495 ± 0.011
2.422 ± 0.005
5.422 ± 0.009
6.029 ± 0.011
10.209 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.495 ± 0.005
4.449 ± 0.009
4.759 ± 0.01
3.659 ± 0.009
5.144 ± 0.01
8.882 ± 0.015
4.931 ± 0.008
6.584 ± 0.01
1.389 ± 0.005
2.894 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here