Marinobacter sp. R17

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Marinobacter; unclassified Marinobacter

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4075 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N2QLJ7|A0A3N2QLJ7_9ALTE Sodium:proton antiporter OS=Marinobacter sp. R17 OX=2484250 GN=EB809_17620 PE=4 SV=1
MM1 pKa = 7.12KK2 pKa = 9.64TLGMLFFLSFLLVGCGGSSSGDD24 pKa = 3.28TSDD27 pKa = 3.53NTSASSDD34 pKa = 3.43SDD36 pKa = 4.61PITGGDD42 pKa = 3.18WYY44 pKa = 11.12QPVPLATWQWQLQGTVDD61 pKa = 3.03TDD63 pKa = 4.11YY64 pKa = 11.56DD65 pKa = 3.95VEE67 pKa = 5.05IYY69 pKa = 10.66DD70 pKa = 4.4IDD72 pKa = 5.24LFDD75 pKa = 6.03SDD77 pKa = 3.98VSLIQSIQATGKK89 pKa = 10.5KK90 pKa = 9.11VICYY94 pKa = 8.54FSAGSYY100 pKa = 9.42EE101 pKa = 3.88NWRR104 pKa = 11.84DD105 pKa = 3.5DD106 pKa = 4.32AGDD109 pKa = 4.12FEE111 pKa = 5.67AADD114 pKa = 4.73LGNSLDD120 pKa = 3.65GWDD123 pKa = 3.64GEE125 pKa = 4.26RR126 pKa = 11.84WLDD129 pKa = 3.36IRR131 pKa = 11.84NEE133 pKa = 3.94NVRR136 pKa = 11.84EE137 pKa = 3.85IMAARR142 pKa = 11.84LDD144 pKa = 3.71LAADD148 pKa = 4.0KK149 pKa = 11.25GCDD152 pKa = 3.46GVEE155 pKa = 4.04PDD157 pKa = 5.34NMDD160 pKa = 4.26GYY162 pKa = 9.77TNDD165 pKa = 3.7PGFDD169 pKa = 3.39FTAADD174 pKa = 3.32QLAYY178 pKa = 10.66NRR180 pKa = 11.84WIANEE185 pKa = 3.55AHH187 pKa = 5.9QRR189 pKa = 11.84TLAVGLKK196 pKa = 10.16NDD198 pKa = 4.05LNQIDD203 pKa = 4.23SLVNYY208 pKa = 10.12FDD210 pKa = 3.86FAVNEE215 pKa = 4.09EE216 pKa = 4.09CFEE219 pKa = 4.01YY220 pKa = 10.86SEE222 pKa = 4.75CDD224 pKa = 3.07RR225 pKa = 11.84LQPFIDD231 pKa = 3.61ANKK234 pKa = 10.0AVLNAEE240 pKa = 4.19YY241 pKa = 10.33QDD243 pKa = 3.89QYY245 pKa = 12.05VNDD248 pKa = 3.71ATARR252 pKa = 11.84AALCTDD258 pKa = 3.32SVNRR262 pKa = 11.84QFSTLVLPLDD272 pKa = 4.17LDD274 pKa = 3.46NSFRR278 pKa = 11.84YY279 pKa = 9.59SCNN282 pKa = 3.16

Molecular weight: 31.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N2R0S2|A0A3N2R0S2_9ALTE Glycine--tRNA ligase alpha subunit OS=Marinobacter sp. R17 OX=2484250 GN=glyQ PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 9.32VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4075

0

4075

1336245

21

4024

327.9

36.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.126 ± 0.04

0.93 ± 0.012

6.312 ± 0.032

6.174 ± 0.042

3.71 ± 0.024

7.833 ± 0.035

2.308 ± 0.02

5.128 ± 0.029

3.405 ± 0.034

10.647 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.523 ± 0.02

3.061 ± 0.021

4.813 ± 0.026

4.069 ± 0.026

6.736 ± 0.037

5.73 ± 0.029

5.268 ± 0.033

7.197 ± 0.032

1.384 ± 0.017

2.648 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski