Streptococcus satellite phage Javan634
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZTN8|A0A4D5ZTN8_9VIRU DNA primase OS=Streptococcus satellite phage Javan634 OX=2558797 GN=JavanS634_0013 PE=4 SV=1
MM1 pKa = 7.73 EE2 pKa = 5.17 YY3 pKa = 10.85 EE4 pKa = 4.73 LLLDD8 pKa = 5.21 DD9 pKa = 5.46 LLEE12 pKa = 4.43 SLSALGVDD20 pKa = 3.59 TSKK23 pKa = 11.49 LNIVEE28 pKa = 4.06 KK29 pKa = 10.06 TDD31 pKa = 3.6 YY32 pKa = 11.16 VDD34 pKa = 3.41 EE35 pKa = 4.38 QEE37 pKa = 5.22 FIIDD41 pKa = 3.62 QRR43 pKa = 11.84 ASKK46 pKa = 10.78 YY47 pKa = 10.9 SYY49 pKa = 9.1 TQLKK53 pKa = 10.81 AEE55 pKa = 4.62 TNHH58 pKa = 5.57 SWVSASFFII67 pKa = 5.56
Molecular weight: 7.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.247
IPC2_protein 4.228
IPC_protein 4.101
Toseland 3.923
ProMoST 4.228
Dawson 4.062
Bjellqvist 4.215
Wikipedia 3.961
Rodwell 3.935
Grimsley 3.834
Solomon 4.05
Lehninger 3.999
Nozaki 4.19
DTASelect 4.342
Thurlkill 3.961
EMBOSS 3.973
Sillero 4.215
Patrickios 3.427
IPC_peptide 4.05
IPC2_peptide 4.19
IPC2.peptide.svr19 4.136
Protein with the highest isoelectric point:
>tr|A0A4D5ZY06|A0A4D5ZY06_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan634 OX=2558797 GN=JavanS634_0015 PE=4 SV=1
MM1 pKa = 7.53 SGTVSISHH9 pKa = 6.57 IAEE12 pKa = 4.11 KK13 pKa = 10.77 GRR15 pKa = 11.84 ARR17 pKa = 11.84 MIWNVKK23 pKa = 9.63 EE24 pKa = 4.03 KK25 pKa = 10.69 DD26 pKa = 3.29 RR27 pKa = 11.84 RR28 pKa = 11.84 PTFTQIKK35 pKa = 9.57 EE36 pKa = 3.9 RR37 pKa = 11.84 QQQKK41 pKa = 10.36 KK42 pKa = 9.91 LKK44 pKa = 9.73 KK45 pKa = 9.36 KK46 pKa = 9.89 RR47 pKa = 11.84 NRR49 pKa = 11.84 GKK51 pKa = 10.6
Molecular weight: 6.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.172
IPC2_protein 10.131
IPC_protein 11.462
Toseland 11.74
ProMoST 12.12
Dawson 11.754
Bjellqvist 11.667
Wikipedia 12.149
Rodwell 11.886
Grimsley 11.784
Solomon 12.164
Lehninger 12.076
Nozaki 11.725
DTASelect 11.667
Thurlkill 11.725
EMBOSS 12.208
Sillero 11.725
Patrickios 11.608
IPC_peptide 12.164
IPC2_peptide 11.096
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
23
0
23
3419
51
494
148.7
17.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.592 ± 0.477
0.702 ± 0.176
6.464 ± 0.48
7.692 ± 0.594
3.802 ± 0.357
4.329 ± 0.418
2.252 ± 0.303
8.774 ± 0.639
9.33 ± 0.59
10.354 ± 0.583
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.194 ± 0.261
6.376 ± 0.474
3.364 ± 0.439
3.451 ± 0.407
5.089 ± 0.392
5.499 ± 0.435
5.937 ± 0.36
3.89 ± 0.416
1.053 ± 0.24
4.855 ± 0.378
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here