Xanthomonas phage phiL7
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C4ML58|C4ML58_9CAUD p58 OS=Xanthomonas phage phiL7 OX=538979 PE=4 SV=1
MM1 pKa = 7.42 MYY3 pKa = 10.47 LVEE6 pKa = 4.23 ITGAYY11 pKa = 10.3 DD12 pKa = 3.61 GFPNVLSDD20 pKa = 3.77 YY21 pKa = 10.79 CLEE24 pKa = 4.77 ADD26 pKa = 4.97 LSNLVRR32 pKa = 11.84 KK33 pKa = 7.91 YY34 pKa = 7.94 TGRR37 pKa = 11.84 YY38 pKa = 7.89 YY39 pKa = 10.66 RR40 pKa = 11.84 IEE42 pKa = 4.46 CDD44 pKa = 3.19 EE45 pKa = 3.96 QDD47 pKa = 3.68 RR48 pKa = 11.84 DD49 pKa = 3.85 DD50 pKa = 3.69 RR51 pKa = 11.84 TIGYY55 pKa = 9.29 VVDD58 pKa = 4.14 VPWRR62 pKa = 11.84 MDD64 pKa = 3.26 DD65 pKa = 3.86 RR66 pKa = 11.84 DD67 pKa = 3.61 WLGTYY72 pKa = 10.78 ALADD76 pKa = 4.23 LLRR79 pKa = 11.84 KK80 pKa = 9.7 AIAMQGEE87 pKa = 4.28 EE88 pKa = 4.76 AIDD91 pKa = 3.62 IAVDD95 pKa = 3.95 YY96 pKa = 10.73 YY97 pKa = 11.54 DD98 pKa = 4.36 EE99 pKa = 4.68 DD100 pKa = 3.66
Molecular weight: 11.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.935
IPC_protein 3.91
Toseland 3.694
ProMoST 4.024
Dawson 3.91
Bjellqvist 4.113
Wikipedia 3.872
Rodwell 3.745
Grimsley 3.605
Solomon 3.897
Lehninger 3.859
Nozaki 4.024
DTASelect 4.291
Thurlkill 3.757
EMBOSS 3.872
Sillero 4.037
Patrickios 1.036
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.937
Protein with the highest isoelectric point:
>tr|C4ML41|C4ML41_9CAUD p41 OS=Xanthomonas phage phiL7 OX=538979 PE=4 SV=1
MM1 pKa = 7.31 TRR3 pKa = 11.84 LTQASLKK10 pKa = 7.63 TWRR13 pKa = 11.84 AGQQTKK19 pKa = 9.07 QAGRR23 pKa = 11.84 CALCGLPLGSSPPHH37 pKa = 6.9 DD38 pKa = 3.44 PVGDD42 pKa = 3.71 HH43 pKa = 6.54 DD44 pKa = 4.48 HH45 pKa = 6.63 RR46 pKa = 11.84 TGAMRR51 pKa = 11.84 GVLHH55 pKa = 6.94 RR56 pKa = 11.84 GCNSLLGCVEE66 pKa = 4.25 NNAPRR71 pKa = 11.84 YY72 pKa = 7.84 GVRR75 pKa = 11.84 DD76 pKa = 2.96 IGVFANGVAQYY87 pKa = 10.94 LRR89 pKa = 11.84 AHH91 pKa = 6.92 IVNTTGLLHH100 pKa = 6.62 PSFRR104 pKa = 11.84 TPDD107 pKa = 3.32 EE108 pKa = 3.76 KK109 pKa = 10.7 RR110 pKa = 11.84 LRR112 pKa = 11.84 RR113 pKa = 11.84 NAKK116 pKa = 9.3 ARR118 pKa = 11.84 KK119 pKa = 8.96 KK120 pKa = 9.79 RR121 pKa = 11.84 AANKK125 pKa = 10.26 GLTDD129 pKa = 3.87 AEE131 pKa = 4.33
Molecular weight: 14.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.633
IPC_protein 10.496
Toseland 10.877
ProMoST 10.628
Dawson 10.935
Bjellqvist 10.672
Wikipedia 11.155
Rodwell 11.096
Grimsley 10.965
Solomon 11.111
Lehninger 11.067
Nozaki 10.877
DTASelect 10.657
Thurlkill 10.862
EMBOSS 11.286
Sillero 10.877
Patrickios 10.847
IPC_peptide 11.125
IPC2_peptide 9.94
IPC2.peptide.svr19 8.665
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
58
0
58
12903
54
1475
222.5
24.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.889 ± 0.594
1.132 ± 0.17
5.464 ± 0.229
5.588 ± 0.3
3.03 ± 0.197
8.548 ± 0.227
2.286 ± 0.298
4.162 ± 0.185
4.58 ± 0.362
8.471 ± 0.294
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.527 ± 0.144
3.534 ± 0.195
4.425 ± 0.265
4.115 ± 0.302
6.448 ± 0.318
6.177 ± 0.364
6.433 ± 0.369
7.239 ± 0.3
1.798 ± 0.153
3.154 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here