Cyanobacterium aponinum (strain PCC 10605)
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3415 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K9Z106|K9Z106_CYAAP Uncharacterized protein OS=Cyanobacterium aponinum (strain PCC 10605) OX=755178 GN=Cyan10605_0267 PE=4 SV=1
MM1 pKa = 7.33 SLVTGDD7 pKa = 3.31 IDD9 pKa = 4.48 GDD11 pKa = 4.21 GIEE14 pKa = 5.0 DD15 pKa = 4.05 LVIGAPNVNNGDD27 pKa = 3.69 GIVYY31 pKa = 8.51 VIRR34 pKa = 11.84 GSYY37 pKa = 9.5 IADD40 pKa = 3.37 NQGQIININSDD51 pKa = 2.87 NSFSDD56 pKa = 3.57 QKK58 pKa = 10.31 NTASNNIGFVFNPTVTGAYY77 pKa = 9.13 FGYY80 pKa = 10.67 AVAVGNFDD88 pKa = 4.12 GNGSLDD94 pKa = 3.87 LAISSPGASKK104 pKa = 11.25 GDD106 pKa = 3.42 GLVYY110 pKa = 10.08 IAHH113 pKa = 6.6 NNSEE117 pKa = 4.44 LQTFATGSNGEE128 pKa = 3.99 NLGYY132 pKa = 10.61 SLAVSQANGKK142 pKa = 9.28 QSFSGNTTIDD152 pKa = 3.72 DD153 pKa = 5.26 LIVGAPSYY161 pKa = 10.97 ASSVSNQWVGADD173 pKa = 3.48 QLPSEE178 pKa = 4.42 NQNLFPNTTSTAIGKK193 pKa = 9.8 VYY195 pKa = 10.84 VFGNNQTTPLYY206 pKa = 10.3 SFTGSILPSTNGTAEE221 pKa = 3.81 NSFTGSALASDD232 pKa = 4.53 DD233 pKa = 3.65 WNLDD237 pKa = 3.16 GARR240 pKa = 11.84 DD241 pKa = 3.78 LAISAPGSDD250 pKa = 3.39 NSDD253 pKa = 3.06 GLVYY257 pKa = 10.21 VVKK260 pKa = 10.56 GGKK263 pKa = 7.04 PTSGDD268 pKa = 3.3 LDD270 pKa = 4.31 SISNLIILGGLPFSKK285 pKa = 9.43 TGSEE289 pKa = 3.84 IASAGDD295 pKa = 3.52 VNGDD299 pKa = 3.29 GYY301 pKa = 11.62 EE302 pKa = 4.23 DD303 pKa = 4.15 FLVGAPQGLMGVGQSYY319 pKa = 11.14 LLFGPLDD326 pKa = 4.36 LDD328 pKa = 3.93 SQGTLFNLNVTATDD342 pKa = 3.45 SKK344 pKa = 11.0 KK345 pKa = 9.05 TFLLNGSQPYY355 pKa = 10.05 QLTGSALSGIGDD367 pKa = 3.82 VNNDD371 pKa = 3.23 NVDD374 pKa = 3.81 DD375 pKa = 5.0 LMITAPNAQQLYY387 pKa = 10.75 AVFGHH392 pKa = 5.97 QWLADD397 pKa = 3.95 DD398 pKa = 5.52 GSIKK402 pKa = 10.61 LADD405 pKa = 3.3 ISADD409 pKa = 3.16 NGFVIDD415 pKa = 3.92 GDD417 pKa = 4.1 LYY419 pKa = 10.83 KK420 pKa = 11.12 ANSKK424 pKa = 10.21 SLNGNGNNVLILGDD438 pKa = 3.58 INGDD442 pKa = 3.31 GFADD446 pKa = 3.76 VLSGGSEE453 pKa = 4.07 DD454 pKa = 3.61 GAVIIFGSSTEE465 pKa = 4.01 NLLDD469 pKa = 3.99 ASVGSNDD476 pKa = 5.62 LILSVANHH484 pKa = 6.9 SIQDD488 pKa = 3.69 FASLGDD494 pKa = 3.79 YY495 pKa = 11.05 NGDD498 pKa = 3.53 GLQDD502 pKa = 3.76 FGVIDD507 pKa = 5.09 DD508 pKa = 5.15 DD509 pKa = 4.55 NNFYY513 pKa = 10.56 IQLGSNNLNSLGNLSLSNPSVTSIIEE539 pKa = 4.48 AIEE542 pKa = 4.17 LGDD545 pKa = 3.94 YY546 pKa = 11.18 NGDD549 pKa = 3.68 GYY551 pKa = 11.41 DD552 pKa = 5.27 DD553 pKa = 3.9 ILLNTSSNSRR563 pKa = 11.84 IYY565 pKa = 10.75 LGNEE569 pKa = 3.58 EE570 pKa = 4.8 GNVNNFLTFNPVGGNSFSDD589 pKa = 3.68 YY590 pKa = 10.38 TFGSIGDD597 pKa = 3.76 IDD599 pKa = 4.27 GDD601 pKa = 4.38 GYY603 pKa = 11.33 QDD605 pKa = 3.21 IATGLPQSNFTIQDD619 pKa = 3.29 AANGQFTIYY628 pKa = 9.92 TNRR631 pKa = 11.84 GNQSILPPVTPSLSNFPSNDD651 pKa = 3.5 YY652 pKa = 11.1 NPTIASYY659 pKa = 10.52 GVPSQQSPVPPNFAVYY675 pKa = 9.87 QGYY678 pKa = 10.43 LYY680 pKa = 7.91 MTFKK684 pKa = 11.35 GNGNDD689 pKa = 4.67 DD690 pKa = 3.86 INVMRR695 pKa = 11.84 SRR697 pKa = 11.84 DD698 pKa = 3.51 GNTWEE703 pKa = 4.12 NQVVLTSLQTPYY715 pKa = 10.92 SPSLVVFEE723 pKa = 5.04 DD724 pKa = 3.28 KK725 pKa = 10.94 LYY727 pKa = 10.81 LIHH730 pKa = 6.63 TFEE733 pKa = 5.87 DD734 pKa = 3.36 STIYY738 pKa = 9.1 ITPFTGDD745 pKa = 3.76 DD746 pKa = 3.35 NSISFGEE753 pKa = 4.3 YY754 pKa = 8.03 TALIADD760 pKa = 5.04 AGTSPTPIVFNNKK773 pKa = 9.63 LYY775 pKa = 10.47 IFYY778 pKa = 10.36 IADD781 pKa = 3.62 GDD783 pKa = 3.98 DD784 pKa = 3.46 QINYY788 pKa = 7.19 ITLSDD793 pKa = 3.91 PANVTEE799 pKa = 4.84 FGYY802 pKa = 11.08 DD803 pKa = 2.99 EE804 pKa = 4.35 FNYY807 pKa = 10.12 INGQQSFDD815 pKa = 3.37 RR816 pKa = 11.84 VGATLTPDD824 pKa = 3.49 GEE826 pKa = 4.57 KK827 pKa = 10.73 LVLAYY832 pKa = 9.88 KK833 pKa = 10.46 NSNKK837 pKa = 9.99 ADD839 pKa = 3.23 IYY841 pKa = 9.79 ITNFDD846 pKa = 3.52 GSNWSEE852 pKa = 3.76 GKK854 pKa = 9.71 IVQDD858 pKa = 3.46 SQNNNNTIEE867 pKa = 4.29 TTDD870 pKa = 3.74 GVSLTTVGNNIYY882 pKa = 10.26 LAYY885 pKa = 10.11 EE886 pKa = 4.13 GTKK889 pKa = 10.69 PNDD892 pKa = 2.95 LRR894 pKa = 11.84 YY895 pKa = 9.69 IYY897 pKa = 10.72 SQDD900 pKa = 4.24 SGNSWEE906 pKa = 4.44 NDD908 pKa = 2.82 RR909 pKa = 11.84 NTPNQLVNGGASLTFFQSSLYY930 pKa = 10.22 FGYY933 pKa = 10.44 SSQVTSNAPLYY944 pKa = 10.41 VSYY947 pKa = 10.96 SEE949 pKa = 5.27 PIYY952 pKa = 10.02 NTNQTQQLGEE962 pKa = 3.92 QLYY965 pKa = 11.05 SIGDD969 pKa = 3.65 FNGDD973 pKa = 3.67 GIEE976 pKa = 4.53 DD977 pKa = 3.89 FAIVAQGYY985 pKa = 9.29 IADD988 pKa = 4.75 LGIIDD993 pKa = 5.37 LSAGLQIRR1001 pKa = 11.84 NNQGAILIYY1010 pKa = 10.14 YY1011 pKa = 9.31 GNANGISNSAKK1022 pKa = 9.64 PDD1024 pKa = 3.5 VVLVNPPVTDD1034 pKa = 3.73 NQSLLYY1040 pKa = 9.97 EE1041 pKa = 4.41 INRR1044 pKa = 11.84 FTAIGDD1050 pKa = 3.82 INGDD1054 pKa = 3.47 GYY1056 pKa = 11.49 DD1057 pKa = 3.94 DD1058 pKa = 4.16 VAVSSPTTKK1067 pKa = 10.6 SNEE1070 pKa = 3.35 GSIFVVFGGSSWQSTYY1086 pKa = 11.3 SATNPFDD1093 pKa = 5.04 LSSLSNNQSNSTTDD1107 pKa = 3.16 NSNTNGFLITGLPASQAGISLSGGEE1132 pKa = 4.65 DD1133 pKa = 3.46 VNGDD1137 pKa = 3.49 GFDD1140 pKa = 4.23 DD1141 pKa = 4.16 FTVGAPGNDD1150 pKa = 3.62 DD1151 pKa = 3.83 DD1152 pKa = 4.23 LTYY1155 pKa = 11.17 VIFGSDD1161 pKa = 2.95 FTQQVNQTGTIGRR1174 pKa = 11.84 DD1175 pKa = 3.15 RR1176 pKa = 11.84 MIGTPTGEE1184 pKa = 3.63 IFIAGEE1190 pKa = 4.16 GNDD1193 pKa = 3.74 RR1194 pKa = 11.84 IYY1196 pKa = 11.46 SNGGVDD1202 pKa = 3.4 VVYY1205 pKa = 10.93 ASVGNDD1211 pKa = 2.95 FVTVNDD1217 pKa = 3.87 TYY1219 pKa = 11.02 FRR1221 pKa = 11.84 RR1222 pKa = 11.84 LDD1224 pKa = 3.66 GGTGIDD1230 pKa = 3.33 TLKK1233 pKa = 9.53 FTGYY1237 pKa = 10.5 NGQNWDD1243 pKa = 4.22 LTTLSPGNRR1252 pKa = 11.84 LRR1254 pKa = 11.84 NFEE1257 pKa = 4.02 ILDD1260 pKa = 3.43 IKK1262 pKa = 11.13 DD1263 pKa = 3.49 YY1264 pKa = 11.74 GEE1266 pKa = 4.01 NTLTLNSLTVTQLSSNNTITVLMDD1290 pKa = 4.47 KK1291 pKa = 10.71 EE1292 pKa = 4.2 DD1293 pKa = 4.0 TLNLSADD1300 pKa = 3.86 FVNEE1304 pKa = 4.45 GIINRR1309 pKa = 11.84 NGEE1312 pKa = 4.21 TYY1314 pKa = 9.69 QKK1316 pKa = 10.91 YY1317 pKa = 9.98 RR1318 pKa = 11.84 IDD1320 pKa = 3.67 GATVLILEE1328 pKa = 5.73 DD1329 pKa = 3.39 IVQTKK1334 pKa = 10.76 AFTPLSLSLYY1344 pKa = 8.35 NTGVDD1349 pKa = 4.88 DD1350 pKa = 6.19 SGTPLNNNSTGDD1362 pKa = 3.41 PHH1364 pKa = 8.21 YY1365 pKa = 10.11 EE1366 pKa = 3.92 LVSAPDD1372 pKa = 3.95 GSSSNILVTNSGDD1385 pKa = 4.16 GISGWISPDD1394 pKa = 3.18 NNNSDD1399 pKa = 3.6 SSPPGDD1405 pKa = 3.39 YY1406 pKa = 10.2 TYY1408 pKa = 10.19 RR1409 pKa = 11.84 TTFNLPDD1416 pKa = 3.97 TVNLSQFFIDD1426 pKa = 5.46 GEE1428 pKa = 4.2 WHH1430 pKa = 6.4 TDD1432 pKa = 3.41 DD1433 pKa = 4.38 EE1434 pKa = 5.76 GITITLNGIEE1444 pKa = 4.85 QYY1446 pKa = 10.73 LASNPNFNTDD1456 pKa = 3.91 FVPFTINEE1464 pKa = 3.95 GFIAGEE1470 pKa = 4.24 NILEE1474 pKa = 4.13 FTVNNAGTEE1483 pKa = 4.0 INPTVLRR1490 pKa = 11.84 VKK1492 pKa = 10.47 FDD1494 pKa = 3.41 NAFDD1498 pKa = 4.39 SNIQFSAPSTNIPQAILNPEE1518 pKa = 4.04 NVSQVSSANNSVVSIDD1534 pKa = 3.4 EE1535 pKa = 4.43 SNNITEE1541 pKa = 4.32 VSSSLFANTSNNEE1554 pKa = 3.87 DD1555 pKa = 3.65 STTNLYY1561 pKa = 7.72 VTNPTVNEE1569 pKa = 3.67 ADD1571 pKa = 3.85 GEE1573 pKa = 4.84 VKK1575 pKa = 9.33 FTIQRR1580 pKa = 11.84 TGNLDD1585 pKa = 3.38 KK1586 pKa = 10.89 YY1587 pKa = 9.41 VQIHH1591 pKa = 5.85 YY1592 pKa = 7.6 VTQDD1596 pKa = 2.69 GRR1598 pKa = 11.84 AKK1600 pKa = 10.57 AGNDD1604 pKa = 3.16 YY1605 pKa = 10.93 HH1606 pKa = 6.17 PTVGKK1611 pKa = 10.51 AIFTPGEE1618 pKa = 3.75 SSINVSVPLILDD1630 pKa = 3.55 DD1631 pKa = 4.75 VYY1633 pKa = 11.37 TGTKK1637 pKa = 10.25 DD1638 pKa = 2.64 IGLFVTLEE1646 pKa = 4.36 KK1647 pKa = 10.59 EE1648 pKa = 4.12 SHH1650 pKa = 6.04 TPLIDD1655 pKa = 3.36 EE1656 pKa = 4.92 FNIHH1660 pKa = 7.06 ADD1662 pKa = 3.65 FNNGIINSWHH1672 pKa = 6.8 HH1673 pKa = 5.51 IQEE1676 pKa = 4.49 EE1677 pKa = 4.72 STHH1680 pKa = 6.34 PLSLINGEE1688 pKa = 4.03 LEE1690 pKa = 4.12 FRR1692 pKa = 11.84 VTATEE1697 pKa = 4.36 GEE1699 pKa = 4.49 AEE1701 pKa = 4.22 VKK1703 pKa = 10.53 LYY1705 pKa = 10.83 FDD1707 pKa = 4.84 GNHH1710 pKa = 5.83 EE1711 pKa = 4.29 FNRR1714 pKa = 11.84 YY1715 pKa = 9.86 YY1716 pKa = 10.29 IFNTQNQRR1724 pKa = 11.84 YY1725 pKa = 7.95 EE1726 pKa = 4.02 VFDD1729 pKa = 4.35 FNGDD1733 pKa = 3.11 TGAEE1737 pKa = 4.21 LFDD1740 pKa = 4.89 EE1741 pKa = 5.92 DD1742 pKa = 5.74 DD1743 pKa = 3.96 DD1744 pKa = 5.16 SNYY1747 pKa = 10.8 EE1748 pKa = 4.01 GVILHH1753 pKa = 6.2 LQDD1756 pKa = 4.48 GSEE1759 pKa = 4.17 HH1760 pKa = 6.9 DD1761 pKa = 4.37 LDD1763 pKa = 4.04 GARR1766 pKa = 11.84 NGVIIKK1772 pKa = 10.28 RR1773 pKa = 11.84 GFFAGGNPPEE1783 pKa = 4.31 TVLNKK1788 pKa = 9.79 PMYY1791 pKa = 10.18 RR1792 pKa = 11.84 FRR1794 pKa = 11.84 NSDD1797 pKa = 3.61 YY1798 pKa = 10.75 DD1799 pKa = 3.54 TGAYY1803 pKa = 10.07 LYY1805 pKa = 10.44 AGEE1808 pKa = 4.86 IEE1810 pKa = 4.33 SEE1812 pKa = 4.64 SIRR1815 pKa = 11.84 EE1816 pKa = 3.89 NYY1818 pKa = 9.48 PNFVEE1823 pKa = 4.5 EE1824 pKa = 4.73 GFAFYY1829 pKa = 11.16 VSTTPQDD1836 pKa = 3.42 GLITFNRR1843 pKa = 11.84 FQNLDD1848 pKa = 3.41 YY1849 pKa = 10.76 PGSYY1853 pKa = 10.3 LYY1855 pKa = 10.87 AEE1857 pKa = 4.38 EE1858 pKa = 4.72 TEE1860 pKa = 4.35 SEE1862 pKa = 4.74 SIRR1865 pKa = 11.84 EE1866 pKa = 3.94 NYY1868 pKa = 9.48 PNFVEE1873 pKa = 4.42 EE1874 pKa = 5.28 GIAFYY1879 pKa = 10.18 AYY1881 pKa = 9.46 PQGSQMADD1889 pKa = 3.29 VISRR1893 pKa = 11.84 FQSNDD1898 pKa = 2.94 LLGSYY1903 pKa = 10.14 LYY1905 pKa = 10.25 TSEE1908 pKa = 5.1 PEE1910 pKa = 3.8 TDD1912 pKa = 3.5 NVISNYY1918 pKa = 10.84 ANFNLEE1924 pKa = 4.17 GIAFEE1929 pKa = 4.58 AVVAA1933 pKa = 4.42
Molecular weight: 208.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.706
IPC_protein 3.77
Toseland 3.528
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.439
Solomon 3.757
Lehninger 3.719
Nozaki 3.859
DTASelect 4.139
Thurlkill 3.579
EMBOSS 3.706
Sillero 3.884
Patrickios 0.642
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.778
Protein with the highest isoelectric point:
>tr|K9Z3X2|K9Z3X2_CYAAP Uncharacterized protein OS=Cyanobacterium aponinum (strain PCC 10605) OX=755178 GN=Cyan10605_0948 PE=4 SV=1
MM1 pKa = 7.74 SKK3 pKa = 9.12 YY4 pKa = 8.82 TLNGTRR10 pKa = 11.84 RR11 pKa = 11.84 KK12 pKa = 9.42 QKK14 pKa = 8.48 RR15 pKa = 11.84 TSGFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 TKK26 pKa = 10.37 NGRR29 pKa = 11.84 KK30 pKa = 9.23 VIQARR35 pKa = 11.84 RR36 pKa = 11.84 NKK38 pKa = 9.03 GRR40 pKa = 11.84 KK41 pKa = 8.78 RR42 pKa = 11.84 LAVV45 pKa = 3.41
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3415
0
3415
1109571
29
4183
324.9
36.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.291 ± 0.04
1.001 ± 0.016
5.078 ± 0.037
6.806 ± 0.044
4.327 ± 0.032
6.24 ± 0.047
1.709 ± 0.023
8.363 ± 0.037
6.221 ± 0.057
10.783 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.823 ± 0.022
5.892 ± 0.059
4.066 ± 0.027
4.807 ± 0.032
4.086 ± 0.031
6.498 ± 0.036
5.384 ± 0.046
5.898 ± 0.033
1.304 ± 0.019
3.424 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here