Colletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422) (Cucumber anthracnose fungus) (Colletotrichum lagenarium)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum; Colletotrichum orbiculare species complex; Colletotrichum orbiculare

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13227 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A484F6J5|A0A484F6J5_COLOR Rhamnogalacturonan acetylesterase OS=Colletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422) OX=1213857 GN=Cob_v013164 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.41FSLSLCLFALASLGVTEE19 pKa = 6.31DD20 pKa = 3.22IDD22 pKa = 3.87EE23 pKa = 4.81HH24 pKa = 7.05DD25 pKa = 4.22VPQACLSDD33 pKa = 3.81CRR35 pKa = 11.84SLLDD39 pKa = 5.1LSAKK43 pKa = 10.42CEE45 pKa = 3.96NQTDD49 pKa = 3.69GDD51 pKa = 4.28SAFNRR56 pKa = 11.84CVCNDD61 pKa = 3.35GGATSSFDD69 pKa = 3.27KK70 pKa = 11.03CAVCAKK76 pKa = 10.4QNGFTSTDD84 pKa = 3.7DD85 pKa = 3.67NDD87 pKa = 3.92VASLMQDD94 pKa = 2.96CGLNFDD100 pKa = 4.91AASATPNTTASVVTSTVTSVSGSDD124 pKa = 3.23TLVQTMTATQTDD136 pKa = 4.48GTSEE140 pKa = 4.29TSGSASGSASTAAAAHH156 pKa = 5.67ATAAAGVVLAGFALGLPVFVV176 pKa = 4.89

Molecular weight: 17.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A484FJZ5|A0A484FJZ5_COLOR Uncharacterized protein OS=Colletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422) OX=1213857 GN=Cob_v008332 PE=4 SV=1
MM1 pKa = 7.4SARR4 pKa = 11.84GGSRR8 pKa = 11.84PPPRR12 pKa = 11.84GRR14 pKa = 11.84PNKK17 pKa = 7.34VTKK20 pKa = 9.56PASQGTTKK28 pKa = 9.0PTPSRR33 pKa = 11.84HH34 pKa = 5.53SPQLPRR40 pKa = 11.84RR41 pKa = 11.84LKK43 pKa = 10.37RR44 pKa = 11.84NSHH47 pKa = 6.01NSSSSSIIIIIIITNINNTIPTTLSTT73 pKa = 3.98

Molecular weight: 7.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13227

0

13227

6122766

66

8752

462.9

50.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.359 ± 0.02

1.27 ± 0.009

5.79 ± 0.017

5.95 ± 0.022

3.776 ± 0.014

7.201 ± 0.021

2.353 ± 0.011

4.494 ± 0.015

4.635 ± 0.021

8.736 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.165 ± 0.01

3.514 ± 0.013

6.123 ± 0.024

3.828 ± 0.015

6.193 ± 0.019

7.863 ± 0.024

5.963 ± 0.031

6.545 ± 0.019

1.535 ± 0.009

2.7 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski