Luteipulveratus mongoliensis
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4674 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1JPQ7|A0A0K1JPQ7_9MICO Peptidase M23 OS=Luteipulveratus mongoliensis OX=571913 GN=VV02_02685 PE=4 SV=1
MM1 pKa = 7.33 VPTGLVGAADD11 pKa = 3.78 EE12 pKa = 4.81 SVAGTADD19 pKa = 3.86 GAWPGDD25 pKa = 3.69 SSVTVADD32 pKa = 4.04 GSGVFGKK39 pKa = 10.03 NLSGLAFEE47 pKa = 5.21 SPDD50 pKa = 3.46 VLWAVKK56 pKa = 10.28 NGPGTLYY63 pKa = 10.9 RR64 pKa = 11.84 LQQDD68 pKa = 3.78 GGTWAPDD75 pKa = 3.9 GEE77 pKa = 4.69 GHH79 pKa = 7.02 ALHH82 pKa = 6.68 YY83 pKa = 10.68 SDD85 pKa = 6.43 GEE87 pKa = 4.12 GDD89 pKa = 3.6 PDD91 pKa = 4.0 AEE93 pKa = 4.18 GVVATADD100 pKa = 3.53 GLFAATEE107 pKa = 4.01 RR108 pKa = 11.84 DD109 pKa = 3.64 NGNEE113 pKa = 3.92 DD114 pKa = 3.47 ASSQKK119 pKa = 10.11 ILRR122 pKa = 11.84 FDD124 pKa = 3.62 PTSSSDD130 pKa = 3.44 SLNATGEE137 pKa = 3.91 WDD139 pKa = 3.68 LTSDD143 pKa = 5.19 LPEE146 pKa = 4.87 SDD148 pKa = 4.32 PNTGIEE154 pKa = 4.65 GITWVPDD161 pKa = 3.38 SYY163 pKa = 11.27 LVSHH167 pKa = 6.71 GFRR170 pKa = 11.84 DD171 pKa = 3.81 EE172 pKa = 4.37 HH173 pKa = 8.4 SGAAYY178 pKa = 10.18 DD179 pKa = 3.74 PSAYY183 pKa = 9.05 PNHH186 pKa = 6.8 GSGLYY191 pKa = 10.43 LVGLEE196 pKa = 4.46 SNGGVYY202 pKa = 10.08 AYY204 pKa = 10.5 ALDD207 pKa = 4.14 LAGSGYY213 pKa = 8.93 TRR215 pKa = 11.84 VATVDD220 pKa = 3.09 TGLPAVMEE228 pKa = 4.51 LEE230 pKa = 4.32 FEE232 pKa = 4.56 PEE234 pKa = 4.06 TGHH237 pKa = 6.87 LWSVCDD243 pKa = 4.28 DD244 pKa = 3.67 TCEE247 pKa = 4.25 GQSKK251 pKa = 10.25 VLDD254 pKa = 4.05 VNDD257 pKa = 3.51 QGAFAVTATYY267 pKa = 10.39 DD268 pKa = 3.55 RR269 pKa = 11.84 PGEE272 pKa = 3.79 MSNYY276 pKa = 8.97 NNEE279 pKa = 3.78 GFAIGPQSMCANGHH293 pKa = 5.99 KK294 pKa = 9.96 PVVWADD300 pKa = 3.48 DD301 pKa = 5.07 DD302 pKa = 5.23 NDD304 pKa = 4.17 DD305 pKa = 4.51 DD306 pKa = 4.04 HH307 pKa = 7.98 ALRR310 pKa = 11.84 SGTLPCAAEE319 pKa = 3.9
Molecular weight: 33.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.795
IPC_protein 3.821
Toseland 3.592
ProMoST 3.961
Dawson 3.808
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.757
Nozaki 3.923
DTASelect 4.177
Thurlkill 3.643
EMBOSS 3.757
Sillero 3.935
Patrickios 1.011
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.85
Protein with the highest isoelectric point:
>tr|A0A0K1JPF7|A0A0K1JPF7_9MICO Bacillolysin OS=Luteipulveratus mongoliensis OX=571913 GN=VV02_03275 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.97 KK16 pKa = 9.33 HH17 pKa = 4.25 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AVLASRR35 pKa = 11.84 RR36 pKa = 11.84 TKK38 pKa = 10.43 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4674
0
4674
1476537
37
2217
315.9
33.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.61 ± 0.042
0.724 ± 0.01
6.384 ± 0.028
5.365 ± 0.037
2.735 ± 0.023
8.978 ± 0.03
2.246 ± 0.018
3.856 ± 0.022
2.356 ± 0.03
10.032 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.971 ± 0.015
1.908 ± 0.019
5.514 ± 0.028
3.192 ± 0.021
7.32 ± 0.041
5.827 ± 0.026
6.316 ± 0.028
8.956 ± 0.036
1.626 ± 0.016
2.084 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here