Santa barbara virus
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A097A5B9|A0A097A5B9_9RHAB GDP polyribonucleotidyltransferase OS=Santa barbara virus OX=1552661 PE=4 SV=1
MM1 pKa = 7.68 APILPFHH8 pKa = 7.35 VDD10 pKa = 3.58 LTAHH14 pKa = 6.96 DD15 pKa = 4.37 ASSAQLIADD24 pKa = 3.95 QIHH27 pKa = 5.91 FLIGFINDD35 pKa = 3.21 LNTPLRR41 pKa = 11.84 LVGIVKK47 pKa = 9.73 QGYY50 pKa = 7.52 WIKK53 pKa = 10.55 IYY55 pKa = 10.79 ALPDD59 pKa = 3.38 QLNKK63 pKa = 10.33 FMMDD67 pKa = 3.86 LVNPLIFGPNSGLII81 pKa = 3.65
Molecular weight: 9.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.627
IPC2_protein 5.512
IPC_protein 5.436
Toseland 6.046
ProMoST 5.83
Dawson 5.753
Bjellqvist 5.728
Wikipedia 5.792
Rodwell 5.728
Grimsley 6.287
Solomon 5.753
Lehninger 5.753
Nozaki 6.02
DTASelect 6.237
Thurlkill 6.237
EMBOSS 6.211
Sillero 6.122
Patrickios 4.075
IPC_peptide 5.766
IPC2_peptide 6.135
IPC2.peptide.svr19 6.181
Protein with the highest isoelectric point:
>tr|A0A097A5A4|A0A097A5A4_9RHAB G protein OS=Santa barbara virus OX=1552661 PE=4 SV=1
MM1 pKa = 7.35 EE2 pKa = 6.07 RR3 pKa = 11.84 GVFDD7 pKa = 5.24 PLVNWLSQASNNVKK21 pKa = 10.23 FYY23 pKa = 10.71 FWVIWLLMLTLGFLFVLSKK42 pKa = 10.92 AIDD45 pKa = 4.64 LISSIILYY53 pKa = 7.77 ATQFYY58 pKa = 10.64 KK59 pKa = 10.86 AFQISLRR66 pKa = 11.84 WITKK70 pKa = 8.75 KK71 pKa = 10.77 CKK73 pKa = 9.74 NCKK76 pKa = 8.62 TNNNQISNKK85 pKa = 9.45 RR86 pKa = 11.84 GLRR89 pKa = 11.84 SAVALVV95 pKa = 3.21
Molecular weight: 11.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.197
IPC2_protein 9.545
IPC_protein 9.56
Toseland 10.321
ProMoST 9.897
Dawson 10.467
Bjellqvist 10.101
Wikipedia 10.584
Rodwell 11.082
Grimsley 10.511
Solomon 10.496
Lehninger 10.467
Nozaki 10.321
DTASelect 10.087
Thurlkill 10.335
EMBOSS 10.701
Sillero 10.379
Patrickios 10.847
IPC_peptide 10.496
IPC2_peptide 8.858
IPC2.peptide.svr19 8.428
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
3932
81
2115
561.7
64.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.967 ± 0.566
1.602 ± 0.261
5.443 ± 0.34
6.612 ± 0.486
4.858 ± 0.53
5.036 ± 0.147
2.162 ± 0.191
8.291 ± 0.35
7.731 ± 0.372
10.224 ± 0.815
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.035 ± 0.218
6.384 ± 0.293
4.4 ± 0.358
3.484 ± 0.215
4.374 ± 0.337
7.884 ± 0.264
5.493 ± 0.359
4.476 ± 0.324
1.958 ± 0.277
3.586 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here