Paenibacillus sp. UNCCL117
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5982 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1K1LLN7|A0A1K1LLN7_9BACL Putative aminopeptidase FrvX OS=Paenibacillus sp. UNCCL117 OX=1502764 GN=SAMN02799630_00191 PE=3 SV=1
MM1 pKa = 7.38 SNKK4 pKa = 9.51 NKK6 pKa = 9.96 HH7 pKa = 5.52 VPPFALKK14 pKa = 10.08 AWRR17 pKa = 11.84 GLLAASLVIAPASYY31 pKa = 9.22 PAAFVHH37 pKa = 7.18 ADD39 pKa = 4.08 LSPHH43 pKa = 6.23 PVHH46 pKa = 7.57 DD47 pKa = 3.94 IVALPGEE54 pKa = 4.31 HH55 pKa = 6.59 MKK57 pKa = 10.88 LQLKK61 pKa = 10.36 DD62 pKa = 4.23 LFDD65 pKa = 4.74 GNSGVFQLSGTEE77 pKa = 4.03 DD78 pKa = 3.68 DD79 pKa = 4.18 EE80 pKa = 4.96 QVVSVNLDD88 pKa = 3.3 THH90 pKa = 6.81 DD91 pKa = 3.78 LQLFFRR97 pKa = 11.84 QPGQATFTVTLNGDD111 pKa = 3.49 SRR113 pKa = 11.84 QVAVNVLEE121 pKa = 4.51 AANEE125 pKa = 4.2 GPMDD129 pKa = 3.76 IGDD132 pKa = 4.14 LVRR135 pKa = 11.84 FINANPEE142 pKa = 3.92 KK143 pKa = 9.82 FTDD146 pKa = 3.59 RR147 pKa = 11.84 SSVSTYY153 pKa = 10.43 LQTVAPGEE161 pKa = 4.28 VQMNHH166 pKa = 6.26 APTVSQATYY175 pKa = 9.91 SLRR178 pKa = 11.84 YY179 pKa = 7.31 TSGYY183 pKa = 7.85 TLDD186 pKa = 3.45 MSAFFNDD193 pKa = 3.42 SDD195 pKa = 4.96 GDD197 pKa = 3.84 TLDD200 pKa = 3.98 YY201 pKa = 11.22 VVLPASMNGIEE212 pKa = 4.35 ASLSGSVLTLTGTQTAKK229 pKa = 10.65 SAFTVYY235 pKa = 11.2 AMDD238 pKa = 4.3 PKK240 pKa = 11.09 GAFASIKK247 pKa = 9.92 LLPNTPPAPLSVTGSVYY264 pKa = 10.64 AAVYY268 pKa = 7.16 EE269 pKa = 4.15 QPQAISLSPYY279 pKa = 9.64 FIDD282 pKa = 4.05 MEE284 pKa = 4.59 GDD286 pKa = 3.26 EE287 pKa = 4.41 LSYY290 pKa = 10.73 RR291 pKa = 11.84 VAGVTVTDD299 pKa = 3.62 YY300 pKa = 10.84 TYY302 pKa = 11.25 NGQLTPLVPFINPGEE317 pKa = 4.39 SILRR321 pKa = 11.84 FSGQLAARR329 pKa = 11.84 TDD331 pKa = 3.28 ISIIAGDD338 pKa = 3.81 GQYY341 pKa = 10.78 EE342 pKa = 4.31 SEE344 pKa = 4.38 PFRR347 pKa = 11.84 LTLDD351 pKa = 4.31 PSPSAYY357 pKa = 9.97 NRR359 pKa = 11.84 PPVGTPLSLSAYY371 pKa = 9.13 KK372 pKa = 10.57 NSLLSSVTLSTYY384 pKa = 10.52 FHH386 pKa = 7.5 DD387 pKa = 5.26 PDD389 pKa = 5.2 GDD391 pKa = 3.95 PLTYY395 pKa = 10.23 SVSPSTSGNVTASIANGILSFSGKK419 pKa = 9.43 IKK421 pKa = 10.89 ANATFTVVATDD432 pKa = 3.03 SHH434 pKa = 6.3 GASTSAAFTYY444 pKa = 10.34 TLDD447 pKa = 3.29 NRR449 pKa = 11.84 APTGSPVTAVVYY461 pKa = 10.86 KK462 pKa = 9.51 NTEE465 pKa = 3.75 LSSIALASHH474 pKa = 6.45 FTDD477 pKa = 5.34 PDD479 pKa = 4.53 GDD481 pKa = 3.57 WLTYY485 pKa = 8.99 TVSPPTSGGVTASITSGVLSFSGQLAANASFIVTAKK521 pKa = 10.3 DD522 pKa = 3.16 AQGAQGSAAFTYY534 pKa = 10.5 VLEE537 pKa = 4.23 NRR539 pKa = 11.84 PPAGTPFTVQVPLYY553 pKa = 6.25 TTPDD557 pKa = 3.47 SVTLATYY564 pKa = 10.43 FSDD567 pKa = 3.97 PDD569 pKa = 3.82 GDD571 pKa = 3.93 ALSYY575 pKa = 10.35 TVTPGAPEE583 pKa = 4.16 SQNGLAASIIDD594 pKa = 4.74 GILSFSGAITGPSEE608 pKa = 4.18 FVVRR612 pKa = 11.84 AYY614 pKa = 10.68 DD615 pKa = 3.48 GGGLFGEE622 pKa = 4.79 AVFAYY627 pKa = 10.09 DD628 pKa = 3.86 IANSAPIAAEE638 pKa = 3.69 PVVSLNVHH646 pKa = 6.0 NEE648 pKa = 3.67 VPPIDD653 pKa = 4.24 LSANFSDD660 pKa = 4.61 PDD662 pKa = 3.78 LDD664 pKa = 3.8 EE665 pKa = 4.5 LTFTVDD671 pKa = 3.11 PLEE674 pKa = 4.27 AAKK677 pKa = 10.69 LPASIEE683 pKa = 3.91 NGYY686 pKa = 10.09 LLIDD690 pKa = 4.47 GMPEE694 pKa = 4.28 DD695 pKa = 3.76 IVSFTVTAMDD705 pKa = 4.27 PSGASASAVYY715 pKa = 10.36 QLTPSNTPPIAEE727 pKa = 4.12 PHH729 pKa = 5.37 TVTGARR735 pKa = 11.84 YY736 pKa = 8.06 HH737 pKa = 6.71 APDD740 pKa = 5.82 PIDD743 pKa = 3.4 LTEE746 pKa = 4.94 FFQDD750 pKa = 4.22 DD751 pKa = 4.57 DD752 pKa = 4.58 GDD754 pKa = 3.93 KK755 pKa = 10.59 LTFSLTSTEE764 pKa = 4.01 EE765 pKa = 4.05 SGGLNAYY772 pKa = 9.64 IEE774 pKa = 4.65 GNHH777 pKa = 6.42 LNFSGAQTATAVFTVTGTDD796 pKa = 3.27 QMGGTISAMFTIEE809 pKa = 5.77 LINQAPTGSSRR820 pKa = 11.84 NEE822 pKa = 3.69 TVFKK826 pKa = 10.86 NDD828 pKa = 3.22 EE829 pKa = 4.26 LVSLSLDD836 pKa = 4.42 DD837 pKa = 5.26 YY838 pKa = 11.04 FTDD841 pKa = 4.15 PDD843 pKa = 4.42 GDD845 pKa = 3.79 EE846 pKa = 4.07 LTYY849 pKa = 10.42 TVSPDD854 pKa = 3.06 EE855 pKa = 4.75 AGGITASIEE864 pKa = 4.19 SGVLYY869 pKa = 10.63 LSGLIQSDD877 pKa = 3.36 ASFTVTATDD886 pKa = 2.77 IGGAEE891 pKa = 3.86 ATATFSYY898 pKa = 10.98 LLANRR903 pKa = 11.84 KK904 pKa = 9.27 PEE906 pKa = 4.16 VVQVPDD912 pKa = 4.93 AISTPRR918 pKa = 11.84 NGALTSIDD926 pKa = 3.25 LSAYY930 pKa = 10.42 FSDD933 pKa = 3.96 PDD935 pKa = 3.65 GDD937 pKa = 3.63 EE938 pKa = 5.25 LYY940 pKa = 11.14 FEE942 pKa = 4.47 VLPNEE947 pKa = 4.23 EE948 pKa = 4.38 VNGITAQINGGVMSFIGSITEE969 pKa = 4.11 TTTFTVRR976 pKa = 11.84 AYY978 pKa = 10.25 DD979 pKa = 3.21 HH980 pKa = 6.8 SEE982 pKa = 3.88 AYY984 pKa = 9.95 EE985 pKa = 3.84 EE986 pKa = 5.19 LEE988 pKa = 4.07 FTFLIVNQAPIAGEE1002 pKa = 3.96 TLITLQAHH1010 pKa = 5.68 NEE1012 pKa = 3.84 VGAIHH1017 pKa = 6.91 LPSNFSDD1024 pKa = 3.75 PDD1026 pKa = 3.6 GDD1028 pKa = 3.95 KK1029 pKa = 11.33 LIFTVDD1035 pKa = 3.41 PAVTGDD1041 pKa = 3.89 LTTSIQSGNLYY1052 pKa = 8.32 ITGVPKK1058 pKa = 10.25 EE1059 pKa = 4.05 QASFTVTATDD1069 pKa = 3.52 EE1070 pKa = 4.42 RR1071 pKa = 11.84 GEE1073 pKa = 4.47 SAWAVYY1079 pKa = 9.01 TIVPVNQAPTGSPVTIAGTWNEE1101 pKa = 4.11 TPATVDD1107 pKa = 3.52 LNDD1110 pKa = 3.56 YY1111 pKa = 10.26 FQDD1114 pKa = 3.54 SDD1116 pKa = 3.65 EE1117 pKa = 5.17 DD1118 pKa = 3.74 EE1119 pKa = 4.78 LLFSIAEE1126 pKa = 4.17 PTTSGGLTAYY1136 pKa = 10.9 LEE1138 pKa = 4.69 GGHH1141 pKa = 5.9 MLSFTGNQTAEE1152 pKa = 3.74 ARR1154 pKa = 11.84 FEE1156 pKa = 3.8 ITAADD1161 pKa = 3.14 IMGGYY1166 pKa = 7.48 EE1167 pKa = 4.15 TITYY1171 pKa = 9.04 TIEE1174 pKa = 3.93 LHH1176 pKa = 5.99 NLPPVGEE1183 pKa = 4.12 VLYY1186 pKa = 10.98 LDD1188 pKa = 3.88 VGRR1191 pKa = 11.84 NSYY1194 pKa = 10.06 AAPISLAEE1202 pKa = 4.28 LFVDD1206 pKa = 4.99 PEE1208 pKa = 4.11 NDD1210 pKa = 3.32 PLTFEE1215 pKa = 4.73 VEE1217 pKa = 4.4 TLSSGNVHH1225 pKa = 7.08 ASISGNLLMFEE1236 pKa = 4.43 GQIEE1240 pKa = 4.12 ADD1242 pKa = 2.85 ASFRR1246 pKa = 11.84 VTATEE1251 pKa = 3.65 YY1252 pKa = 10.56 RR1253 pKa = 11.84 YY1254 pKa = 10.66 DD1255 pKa = 3.44 QAAPLSATADD1265 pKa = 2.99 IHH1267 pKa = 6.85 LRR1269 pKa = 11.84 AVNSAPEE1276 pKa = 4.04 TASPVITDD1284 pKa = 3.87 YY1285 pKa = 11.55 YY1286 pKa = 10.35 HH1287 pKa = 6.32 YY1288 pKa = 10.39 LYY1290 pKa = 10.86 GEE1292 pKa = 4.3 AYY1294 pKa = 9.5 IYY1296 pKa = 10.1 PAVTLAHH1303 pKa = 7.13 LFLDD1307 pKa = 4.25 PNGDD1311 pKa = 3.82 PLTFSLKK1318 pKa = 10.72 DD1319 pKa = 3.46 GANSGLSEE1327 pKa = 4.12 VTVNGVKK1334 pKa = 9.36 ATIMPDD1340 pKa = 2.94 NEE1342 pKa = 4.66 KK1343 pKa = 10.66 IMLYY1347 pKa = 10.58 FGGNPTNVDD1356 pKa = 3.27 EE1357 pKa = 5.7 AIEE1360 pKa = 4.26 FTVVAADD1367 pKa = 3.75 GQGAEE1372 pKa = 4.25 TPLTYY1377 pKa = 10.68 RR1378 pKa = 11.84 LAFNHH1383 pKa = 6.4 APEE1386 pKa = 4.65 LVDD1389 pKa = 3.98 PEE1391 pKa = 4.67 GGSEE1395 pKa = 3.96 GLAYY1399 pKa = 10.56 VYY1401 pKa = 10.04 IDD1403 pKa = 3.14 HH1404 pKa = 7.24 TGTIEE1409 pKa = 5.27 DD1410 pKa = 4.67 LDD1412 pKa = 4.99 LNTLVDD1418 pKa = 5.31 DD1419 pKa = 4.98 PDD1421 pKa = 4.74 GDD1423 pKa = 4.15 PLAFTYY1429 pKa = 11.12 YY1430 pKa = 10.59 EE1431 pKa = 4.68 DD1432 pKa = 5.23 DD1433 pKa = 3.9 SYY1435 pKa = 11.97 TDD1437 pKa = 3.71 GLSVTLSGSTLSFEE1451 pKa = 4.12 GRR1453 pKa = 11.84 LNRR1456 pKa = 11.84 EE1457 pKa = 3.78 TPEE1460 pKa = 4.4 PEE1462 pKa = 4.39 LALHH1466 pKa = 6.33 LQADD1470 pKa = 3.9 DD1471 pKa = 4.25 GKK1473 pKa = 10.52 NGRR1476 pKa = 11.84 IDD1478 pKa = 3.72 FVLLARR1484 pKa = 11.84 YY1485 pKa = 9.39 RR1486 pKa = 11.84 SIPVASEE1493 pKa = 3.79 PEE1495 pKa = 4.05 VRR1497 pKa = 11.84 VSYY1500 pKa = 10.73 LKK1502 pKa = 9.95 EE1503 pKa = 3.84 QPAGALALSSVYY1515 pKa = 10.75 NRR1517 pKa = 11.84 FYY1519 pKa = 10.9 ATDD1522 pKa = 3.82 GNEE1525 pKa = 4.02 LFYY1528 pKa = 10.65 EE1529 pKa = 4.33 IYY1531 pKa = 10.31 EE1532 pKa = 4.31 PEE1534 pKa = 4.73 GEE1536 pKa = 4.28 PLPDD1540 pKa = 4.48 GYY1542 pKa = 10.53 HH1543 pKa = 7.08 AEE1545 pKa = 3.93 LAYY1548 pKa = 9.91 PDD1550 pKa = 4.32 GLLHH1554 pKa = 6.05 VQLSKK1559 pKa = 11.15 DD1560 pKa = 3.2 SSVEE1564 pKa = 3.74 VEE1566 pKa = 4.27 TPYY1569 pKa = 10.75 VPVRR1573 pKa = 11.84 VRR1575 pKa = 11.84 AHH1577 pKa = 6.28 GAHH1580 pKa = 6.7 GFAEE1584 pKa = 4.11 IVYY1587 pKa = 8.81 VFQLNQAPASLLPSNVDD1604 pKa = 3.28 IYY1606 pKa = 11.11 PEE1608 pKa = 3.88 IGILNQRR1615 pKa = 11.84 EE1616 pKa = 4.21 FFMDD1620 pKa = 3.98 PDD1622 pKa = 4.91 GGDD1625 pKa = 3.2 SFTVEE1630 pKa = 3.63 MSITDD1635 pKa = 3.3 RR1636 pKa = 11.84 GYY1638 pKa = 10.86 DD1639 pKa = 3.57 GQNYY1643 pKa = 7.99 WLGITGGSYY1652 pKa = 10.63 EE1653 pKa = 5.02 LIGLNPYY1660 pKa = 10.56 LEE1662 pKa = 5.53 DD1663 pKa = 3.94 GLNGIISFTATDD1675 pKa = 3.15 NHH1677 pKa = 5.83 TQSSQQTVEE1686 pKa = 3.8 FHH1688 pKa = 5.7 KK1689 pKa = 9.53 TAASEE1694 pKa = 4.3 VIQMPDD1700 pKa = 2.73 KK1701 pKa = 10.54 RR1702 pKa = 11.84 LSAGTEE1708 pKa = 3.91 LTLKK1712 pKa = 10.54 DD1713 pKa = 3.47 LSTRR1717 pKa = 11.84 YY1718 pKa = 10.41 SLDD1721 pKa = 3.55 TIHH1724 pKa = 7.1 KK1725 pKa = 8.97 FKK1727 pKa = 10.97 ASSDD1731 pKa = 3.84 DD1732 pKa = 3.7 PEE1734 pKa = 6.17 GMLVSISNDD1743 pKa = 3.45 GQDD1746 pKa = 3.13 LTLAAEE1752 pKa = 4.09 HH1753 pKa = 6.75 AGTYY1757 pKa = 10.0 RR1758 pKa = 11.84 ITLIGTAADD1767 pKa = 3.46 DD1768 pKa = 3.85 TGFIDD1773 pKa = 3.5 QFYY1776 pKa = 8.91 VTVTEE1781 pKa = 4.17 PEE1783 pKa = 3.99 IFGSSLTISNPFGTEE1798 pKa = 3.8 AIVNRR1803 pKa = 11.84 DD1804 pKa = 3.3 SVEE1807 pKa = 4.01 SEE1809 pKa = 3.95 NANFAAVLEE1818 pKa = 4.86 DD1819 pKa = 3.91 EE1820 pKa = 5.35 ADD1822 pKa = 3.86 PDD1824 pKa = 4.13 LLQLQVSVDD1833 pKa = 3.17 EE1834 pKa = 4.78 GAAFLEE1840 pKa = 3.97 NTYY1843 pKa = 10.71 FILYY1847 pKa = 10.33 SNGAIYY1853 pKa = 10.16 KK1854 pKa = 9.87 IRR1856 pKa = 11.84 FYY1858 pKa = 11.56 SNTTAA1863 pKa = 4.53
Molecular weight: 199.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.948
IPC_protein 3.973
Toseland 3.77
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.859
Rodwell 3.808
Grimsley 3.681
Solomon 3.948
Lehninger 3.91
Nozaki 4.05
DTASelect 4.279
Thurlkill 3.795
EMBOSS 3.872
Sillero 4.088
Patrickios 1.074
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 3.969
Protein with the highest isoelectric point:
>tr|A0A1K1Q3S0|A0A1K1Q3S0_9BACL Ribosomal RNA large subunit methyltransferase H OS=Paenibacillus sp. UNCCL117 OX=1502764 GN=rlmH PE=3 SV=1
MM1 pKa = 7.9 RR2 pKa = 11.84 PTFKK6 pKa = 10.87 PNVRR10 pKa = 11.84 KK11 pKa = 9.84 RR12 pKa = 11.84 KK13 pKa = 8.89 KK14 pKa = 8.64 VHH16 pKa = 5.57 GFRR19 pKa = 11.84 KK20 pKa = 10.01 RR21 pKa = 11.84 MSTKK25 pKa = 10.18 NGRR28 pKa = 11.84 KK29 pKa = 8.87 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.2 GRR39 pKa = 11.84 KK40 pKa = 8.79 VLSAA44 pKa = 4.05
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5982
0
5982
2115940
9
3347
353.7
39.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.233 ± 0.039
0.72 ± 0.01
5.063 ± 0.024
6.653 ± 0.036
3.954 ± 0.023
7.804 ± 0.034
2.045 ± 0.017
5.921 ± 0.028
4.883 ± 0.032
10.262 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.704 ± 0.017
3.384 ± 0.026
4.347 ± 0.02
3.906 ± 0.02
5.582 ± 0.033
6.221 ± 0.025
5.415 ± 0.029
7.022 ± 0.026
1.354 ± 0.012
3.525 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here