Paenibacillus sp. UNCCL117

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5982 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1K1LLN7|A0A1K1LLN7_9BACL Putative aminopeptidase FrvX OS=Paenibacillus sp. UNCCL117 OX=1502764 GN=SAMN02799630_00191 PE=3 SV=1
MM1 pKa = 7.38SNKK4 pKa = 9.51NKK6 pKa = 9.96HH7 pKa = 5.52VPPFALKK14 pKa = 10.08AWRR17 pKa = 11.84GLLAASLVIAPASYY31 pKa = 9.22PAAFVHH37 pKa = 7.18ADD39 pKa = 4.08LSPHH43 pKa = 6.23PVHH46 pKa = 7.57DD47 pKa = 3.94IVALPGEE54 pKa = 4.31HH55 pKa = 6.59MKK57 pKa = 10.88LQLKK61 pKa = 10.36DD62 pKa = 4.23LFDD65 pKa = 4.74GNSGVFQLSGTEE77 pKa = 4.03DD78 pKa = 3.68DD79 pKa = 4.18EE80 pKa = 4.96QVVSVNLDD88 pKa = 3.3THH90 pKa = 6.81DD91 pKa = 3.78LQLFFRR97 pKa = 11.84QPGQATFTVTLNGDD111 pKa = 3.49SRR113 pKa = 11.84QVAVNVLEE121 pKa = 4.51AANEE125 pKa = 4.2GPMDD129 pKa = 3.76IGDD132 pKa = 4.14LVRR135 pKa = 11.84FINANPEE142 pKa = 3.92KK143 pKa = 9.82FTDD146 pKa = 3.59RR147 pKa = 11.84SSVSTYY153 pKa = 10.43LQTVAPGEE161 pKa = 4.28VQMNHH166 pKa = 6.26APTVSQATYY175 pKa = 9.91SLRR178 pKa = 11.84YY179 pKa = 7.31TSGYY183 pKa = 7.85TLDD186 pKa = 3.45MSAFFNDD193 pKa = 3.42SDD195 pKa = 4.96GDD197 pKa = 3.84TLDD200 pKa = 3.98YY201 pKa = 11.22VVLPASMNGIEE212 pKa = 4.35ASLSGSVLTLTGTQTAKK229 pKa = 10.65SAFTVYY235 pKa = 11.2AMDD238 pKa = 4.3PKK240 pKa = 11.09GAFASIKK247 pKa = 9.92LLPNTPPAPLSVTGSVYY264 pKa = 10.64AAVYY268 pKa = 7.16EE269 pKa = 4.15QPQAISLSPYY279 pKa = 9.64FIDD282 pKa = 4.05MEE284 pKa = 4.59GDD286 pKa = 3.26EE287 pKa = 4.41LSYY290 pKa = 10.73RR291 pKa = 11.84VAGVTVTDD299 pKa = 3.62YY300 pKa = 10.84TYY302 pKa = 11.25NGQLTPLVPFINPGEE317 pKa = 4.39SILRR321 pKa = 11.84FSGQLAARR329 pKa = 11.84TDD331 pKa = 3.28ISIIAGDD338 pKa = 3.81GQYY341 pKa = 10.78EE342 pKa = 4.31SEE344 pKa = 4.38PFRR347 pKa = 11.84LTLDD351 pKa = 4.31PSPSAYY357 pKa = 9.97NRR359 pKa = 11.84PPVGTPLSLSAYY371 pKa = 9.13KK372 pKa = 10.57NSLLSSVTLSTYY384 pKa = 10.52FHH386 pKa = 7.5DD387 pKa = 5.26PDD389 pKa = 5.2GDD391 pKa = 3.95PLTYY395 pKa = 10.23SVSPSTSGNVTASIANGILSFSGKK419 pKa = 9.43IKK421 pKa = 10.89ANATFTVVATDD432 pKa = 3.03SHH434 pKa = 6.3GASTSAAFTYY444 pKa = 10.34TLDD447 pKa = 3.29NRR449 pKa = 11.84APTGSPVTAVVYY461 pKa = 10.86KK462 pKa = 9.51NTEE465 pKa = 3.75LSSIALASHH474 pKa = 6.45FTDD477 pKa = 5.34PDD479 pKa = 4.53GDD481 pKa = 3.57WLTYY485 pKa = 8.99TVSPPTSGGVTASITSGVLSFSGQLAANASFIVTAKK521 pKa = 10.3DD522 pKa = 3.16AQGAQGSAAFTYY534 pKa = 10.5VLEE537 pKa = 4.23NRR539 pKa = 11.84PPAGTPFTVQVPLYY553 pKa = 6.25TTPDD557 pKa = 3.47SVTLATYY564 pKa = 10.43FSDD567 pKa = 3.97PDD569 pKa = 3.82GDD571 pKa = 3.93ALSYY575 pKa = 10.35TVTPGAPEE583 pKa = 4.16SQNGLAASIIDD594 pKa = 4.74GILSFSGAITGPSEE608 pKa = 4.18FVVRR612 pKa = 11.84AYY614 pKa = 10.68DD615 pKa = 3.48GGGLFGEE622 pKa = 4.79AVFAYY627 pKa = 10.09DD628 pKa = 3.86IANSAPIAAEE638 pKa = 3.69PVVSLNVHH646 pKa = 6.0NEE648 pKa = 3.67VPPIDD653 pKa = 4.24LSANFSDD660 pKa = 4.61PDD662 pKa = 3.78LDD664 pKa = 3.8EE665 pKa = 4.5LTFTVDD671 pKa = 3.11PLEE674 pKa = 4.27AAKK677 pKa = 10.69LPASIEE683 pKa = 3.91NGYY686 pKa = 10.09LLIDD690 pKa = 4.47GMPEE694 pKa = 4.28DD695 pKa = 3.76IVSFTVTAMDD705 pKa = 4.27PSGASASAVYY715 pKa = 10.36QLTPSNTPPIAEE727 pKa = 4.12PHH729 pKa = 5.37TVTGARR735 pKa = 11.84YY736 pKa = 8.06HH737 pKa = 6.71APDD740 pKa = 5.82PIDD743 pKa = 3.4LTEE746 pKa = 4.94FFQDD750 pKa = 4.22DD751 pKa = 4.57DD752 pKa = 4.58GDD754 pKa = 3.93KK755 pKa = 10.59LTFSLTSTEE764 pKa = 4.01EE765 pKa = 4.05SGGLNAYY772 pKa = 9.64IEE774 pKa = 4.65GNHH777 pKa = 6.42LNFSGAQTATAVFTVTGTDD796 pKa = 3.27QMGGTISAMFTIEE809 pKa = 5.77LINQAPTGSSRR820 pKa = 11.84NEE822 pKa = 3.69TVFKK826 pKa = 10.86NDD828 pKa = 3.22EE829 pKa = 4.26LVSLSLDD836 pKa = 4.42DD837 pKa = 5.26YY838 pKa = 11.04FTDD841 pKa = 4.15PDD843 pKa = 4.42GDD845 pKa = 3.79EE846 pKa = 4.07LTYY849 pKa = 10.42TVSPDD854 pKa = 3.06EE855 pKa = 4.75AGGITASIEE864 pKa = 4.19SGVLYY869 pKa = 10.63LSGLIQSDD877 pKa = 3.36ASFTVTATDD886 pKa = 2.77IGGAEE891 pKa = 3.86ATATFSYY898 pKa = 10.98LLANRR903 pKa = 11.84KK904 pKa = 9.27PEE906 pKa = 4.16VVQVPDD912 pKa = 4.93AISTPRR918 pKa = 11.84NGALTSIDD926 pKa = 3.25LSAYY930 pKa = 10.42FSDD933 pKa = 3.96PDD935 pKa = 3.65GDD937 pKa = 3.63EE938 pKa = 5.25LYY940 pKa = 11.14FEE942 pKa = 4.47VLPNEE947 pKa = 4.23EE948 pKa = 4.38VNGITAQINGGVMSFIGSITEE969 pKa = 4.11TTTFTVRR976 pKa = 11.84AYY978 pKa = 10.25DD979 pKa = 3.21HH980 pKa = 6.8SEE982 pKa = 3.88AYY984 pKa = 9.95EE985 pKa = 3.84EE986 pKa = 5.19LEE988 pKa = 4.07FTFLIVNQAPIAGEE1002 pKa = 3.96TLITLQAHH1010 pKa = 5.68NEE1012 pKa = 3.84VGAIHH1017 pKa = 6.91LPSNFSDD1024 pKa = 3.75PDD1026 pKa = 3.6GDD1028 pKa = 3.95KK1029 pKa = 11.33LIFTVDD1035 pKa = 3.41PAVTGDD1041 pKa = 3.89LTTSIQSGNLYY1052 pKa = 8.32ITGVPKK1058 pKa = 10.25EE1059 pKa = 4.05QASFTVTATDD1069 pKa = 3.52EE1070 pKa = 4.42RR1071 pKa = 11.84GEE1073 pKa = 4.47SAWAVYY1079 pKa = 9.01TIVPVNQAPTGSPVTIAGTWNEE1101 pKa = 4.11TPATVDD1107 pKa = 3.52LNDD1110 pKa = 3.56YY1111 pKa = 10.26FQDD1114 pKa = 3.54SDD1116 pKa = 3.65EE1117 pKa = 5.17DD1118 pKa = 3.74EE1119 pKa = 4.78LLFSIAEE1126 pKa = 4.17PTTSGGLTAYY1136 pKa = 10.9LEE1138 pKa = 4.69GGHH1141 pKa = 5.9MLSFTGNQTAEE1152 pKa = 3.74ARR1154 pKa = 11.84FEE1156 pKa = 3.8ITAADD1161 pKa = 3.14IMGGYY1166 pKa = 7.48EE1167 pKa = 4.15TITYY1171 pKa = 9.04TIEE1174 pKa = 3.93LHH1176 pKa = 5.99NLPPVGEE1183 pKa = 4.12VLYY1186 pKa = 10.98LDD1188 pKa = 3.88VGRR1191 pKa = 11.84NSYY1194 pKa = 10.06AAPISLAEE1202 pKa = 4.28LFVDD1206 pKa = 4.99PEE1208 pKa = 4.11NDD1210 pKa = 3.32PLTFEE1215 pKa = 4.73VEE1217 pKa = 4.4TLSSGNVHH1225 pKa = 7.08ASISGNLLMFEE1236 pKa = 4.43GQIEE1240 pKa = 4.12ADD1242 pKa = 2.85ASFRR1246 pKa = 11.84VTATEE1251 pKa = 3.65YY1252 pKa = 10.56RR1253 pKa = 11.84YY1254 pKa = 10.66DD1255 pKa = 3.44QAAPLSATADD1265 pKa = 2.99IHH1267 pKa = 6.85LRR1269 pKa = 11.84AVNSAPEE1276 pKa = 4.04TASPVITDD1284 pKa = 3.87YY1285 pKa = 11.55YY1286 pKa = 10.35HH1287 pKa = 6.32YY1288 pKa = 10.39LYY1290 pKa = 10.86GEE1292 pKa = 4.3AYY1294 pKa = 9.5IYY1296 pKa = 10.1PAVTLAHH1303 pKa = 7.13LFLDD1307 pKa = 4.25PNGDD1311 pKa = 3.82PLTFSLKK1318 pKa = 10.72DD1319 pKa = 3.46GANSGLSEE1327 pKa = 4.12VTVNGVKK1334 pKa = 9.36ATIMPDD1340 pKa = 2.94NEE1342 pKa = 4.66KK1343 pKa = 10.66IMLYY1347 pKa = 10.58FGGNPTNVDD1356 pKa = 3.27EE1357 pKa = 5.7AIEE1360 pKa = 4.26FTVVAADD1367 pKa = 3.75GQGAEE1372 pKa = 4.25TPLTYY1377 pKa = 10.68RR1378 pKa = 11.84LAFNHH1383 pKa = 6.4APEE1386 pKa = 4.65LVDD1389 pKa = 3.98PEE1391 pKa = 4.67GGSEE1395 pKa = 3.96GLAYY1399 pKa = 10.56VYY1401 pKa = 10.04IDD1403 pKa = 3.14HH1404 pKa = 7.24TGTIEE1409 pKa = 5.27DD1410 pKa = 4.67LDD1412 pKa = 4.99LNTLVDD1418 pKa = 5.31DD1419 pKa = 4.98PDD1421 pKa = 4.74GDD1423 pKa = 4.15PLAFTYY1429 pKa = 11.12YY1430 pKa = 10.59EE1431 pKa = 4.68DD1432 pKa = 5.23DD1433 pKa = 3.9SYY1435 pKa = 11.97TDD1437 pKa = 3.71GLSVTLSGSTLSFEE1451 pKa = 4.12GRR1453 pKa = 11.84LNRR1456 pKa = 11.84EE1457 pKa = 3.78TPEE1460 pKa = 4.4PEE1462 pKa = 4.39LALHH1466 pKa = 6.33LQADD1470 pKa = 3.9DD1471 pKa = 4.25GKK1473 pKa = 10.52NGRR1476 pKa = 11.84IDD1478 pKa = 3.72FVLLARR1484 pKa = 11.84YY1485 pKa = 9.39RR1486 pKa = 11.84SIPVASEE1493 pKa = 3.79PEE1495 pKa = 4.05VRR1497 pKa = 11.84VSYY1500 pKa = 10.73LKK1502 pKa = 9.95EE1503 pKa = 3.84QPAGALALSSVYY1515 pKa = 10.75NRR1517 pKa = 11.84FYY1519 pKa = 10.9ATDD1522 pKa = 3.82GNEE1525 pKa = 4.02LFYY1528 pKa = 10.65EE1529 pKa = 4.33IYY1531 pKa = 10.31EE1532 pKa = 4.31PEE1534 pKa = 4.73GEE1536 pKa = 4.28PLPDD1540 pKa = 4.48GYY1542 pKa = 10.53HH1543 pKa = 7.08AEE1545 pKa = 3.93LAYY1548 pKa = 9.91PDD1550 pKa = 4.32GLLHH1554 pKa = 6.05VQLSKK1559 pKa = 11.15DD1560 pKa = 3.2SSVEE1564 pKa = 3.74VEE1566 pKa = 4.27TPYY1569 pKa = 10.75VPVRR1573 pKa = 11.84VRR1575 pKa = 11.84AHH1577 pKa = 6.28GAHH1580 pKa = 6.7GFAEE1584 pKa = 4.11IVYY1587 pKa = 8.81VFQLNQAPASLLPSNVDD1604 pKa = 3.28IYY1606 pKa = 11.11PEE1608 pKa = 3.88IGILNQRR1615 pKa = 11.84EE1616 pKa = 4.21FFMDD1620 pKa = 3.98PDD1622 pKa = 4.91GGDD1625 pKa = 3.2SFTVEE1630 pKa = 3.63MSITDD1635 pKa = 3.3RR1636 pKa = 11.84GYY1638 pKa = 10.86DD1639 pKa = 3.57GQNYY1643 pKa = 7.99WLGITGGSYY1652 pKa = 10.63EE1653 pKa = 5.02LIGLNPYY1660 pKa = 10.56LEE1662 pKa = 5.53DD1663 pKa = 3.94GLNGIISFTATDD1675 pKa = 3.15NHH1677 pKa = 5.83TQSSQQTVEE1686 pKa = 3.8FHH1688 pKa = 5.7KK1689 pKa = 9.53TAASEE1694 pKa = 4.3VIQMPDD1700 pKa = 2.73KK1701 pKa = 10.54RR1702 pKa = 11.84LSAGTEE1708 pKa = 3.91LTLKK1712 pKa = 10.54DD1713 pKa = 3.47LSTRR1717 pKa = 11.84YY1718 pKa = 10.41SLDD1721 pKa = 3.55TIHH1724 pKa = 7.1KK1725 pKa = 8.97FKK1727 pKa = 10.97ASSDD1731 pKa = 3.84DD1732 pKa = 3.7PEE1734 pKa = 6.17GMLVSISNDD1743 pKa = 3.45GQDD1746 pKa = 3.13LTLAAEE1752 pKa = 4.09HH1753 pKa = 6.75AGTYY1757 pKa = 10.0RR1758 pKa = 11.84ITLIGTAADD1767 pKa = 3.46DD1768 pKa = 3.85TGFIDD1773 pKa = 3.5QFYY1776 pKa = 8.91VTVTEE1781 pKa = 4.17PEE1783 pKa = 3.99IFGSSLTISNPFGTEE1798 pKa = 3.8AIVNRR1803 pKa = 11.84DD1804 pKa = 3.3SVEE1807 pKa = 4.01SEE1809 pKa = 3.95NANFAAVLEE1818 pKa = 4.86DD1819 pKa = 3.91EE1820 pKa = 5.35ADD1822 pKa = 3.86PDD1824 pKa = 4.13LLQLQVSVDD1833 pKa = 3.17EE1834 pKa = 4.78GAAFLEE1840 pKa = 3.97NTYY1843 pKa = 10.71FILYY1847 pKa = 10.33SNGAIYY1853 pKa = 10.16KK1854 pKa = 9.87IRR1856 pKa = 11.84FYY1858 pKa = 11.56SNTTAA1863 pKa = 4.53

Molecular weight:
199.18 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1K1Q3S0|A0A1K1Q3S0_9BACL Ribosomal RNA large subunit methyltransferase H OS=Paenibacillus sp. UNCCL117 OX=1502764 GN=rlmH PE=3 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.87PNVRR10 pKa = 11.84KK11 pKa = 9.84RR12 pKa = 11.84KK13 pKa = 8.89KK14 pKa = 8.64VHH16 pKa = 5.57GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight:
5.19 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5982

0

5982

2115940

9

3347

353.7

39.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.233 ± 0.039

0.72 ± 0.01

5.063 ± 0.024

6.653 ± 0.036

3.954 ± 0.023

7.804 ± 0.034

2.045 ± 0.017

5.921 ± 0.028

4.883 ± 0.032

10.262 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.704 ± 0.017

3.384 ± 0.026

4.347 ± 0.02

3.906 ± 0.02

5.582 ± 0.033

6.221 ± 0.025

5.415 ± 0.029

7.022 ± 0.026

1.354 ± 0.012

3.525 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski