Shahe isopoda virus 3
Average proteome isoelectric point is 7.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KL89|A0A1L3KL89_9VIRU Uncharacterized protein OS=Shahe isopoda virus 3 OX=1923423 PE=4 SV=1
MM1 pKa = 7.55 AAPVGANYY9 pKa = 7.49 FTQAALNTLAYY20 pKa = 9.94 VAGIPGLDD28 pKa = 3.49 PRR30 pKa = 11.84 DD31 pKa = 4.74 LIMQGHH37 pKa = 7.07 PEE39 pKa = 3.91 LFEE42 pKa = 4.24 YY43 pKa = 10.48 EE44 pKa = 4.49 CLALPGMTCGEE55 pKa = 4.18 SALRR59 pKa = 11.84 LASAAVWSEE68 pKa = 3.85 TNLKK72 pKa = 10.13 KK73 pKa = 10.34 KK74 pKa = 10.38 RR75 pKa = 11.84 QDD77 pKa = 3.13 RR78 pKa = 11.84 DD79 pKa = 3.41 PPPFLLHH86 pKa = 6.23 GVAGALTTLQMVRR99 pKa = 11.84 RR100 pKa = 11.84 LNRR103 pKa = 11.84 LSMGSFPIYY112 pKa = 9.95 RR113 pKa = 11.84 YY114 pKa = 10.44 AGLDD118 pKa = 3.32 VLGAPMFDD126 pKa = 4.79 LISGKK131 pKa = 9.25 PRR133 pKa = 11.84 ADD135 pKa = 3.17 NYY137 pKa = 11.26 ACVFVPQNLVLVPAFPVAHH156 pKa = 5.82 WTFTTILPPPDD167 pKa = 3.33 VPDD170 pKa = 3.75 RR171 pKa = 11.84 LVVGLPTLIYY181 pKa = 10.03 TRR183 pKa = 11.84 LPLAPQMDD191 pKa = 4.69 PVPGQIVFWRR201 pKa = 11.84 ARR203 pKa = 11.84 VTPEE207 pKa = 3.41 NAEE210 pKa = 3.98 AFAAAAAVGVACKK223 pKa = 10.51 CPWIQVCDD231 pKa = 4.09 HH232 pKa = 5.8 EE233 pKa = 4.75 QAWLAVNPFFSRR245 pKa = 11.84 VTCADD250 pKa = 3.64 SACMEE255 pKa = 4.27 GSSYY259 pKa = 11.87 AMVLKK264 pKa = 9.61 TLSPDD269 pKa = 4.22 FILRR273 pKa = 11.84 RR274 pKa = 11.84 IPNNGVKK281 pKa = 9.92 VLQHH285 pKa = 4.82 NTVLKK290 pKa = 9.67 EE291 pKa = 4.05 EE292 pKa = 4.41 NVFGCPTIRR301 pKa = 11.84 SEE303 pKa = 5.14 RR304 pKa = 11.84 FFNTLATAFGSLEE317 pKa = 3.98 LQPYY321 pKa = 9.43 QSVAEE326 pKa = 4.0 RR327 pKa = 11.84 DD328 pKa = 3.08 IYY330 pKa = 10.29 PYY332 pKa = 10.48 RR333 pKa = 11.84 FRR335 pKa = 11.84 VYY337 pKa = 9.24 TYY339 pKa = 10.05 GRR341 pKa = 11.84 CVEE344 pKa = 4.27 HH345 pKa = 5.83 ATMYY349 pKa = 11.68 NMFKK353 pKa = 9.83 TLCLDD358 pKa = 3.66 TASMLSPYY366 pKa = 9.18 TFQRR370 pKa = 11.84 EE371 pKa = 4.24 FEE373 pKa = 4.37 VEE375 pKa = 3.99 PVAPAKK381 pKa = 10.26 PWGEE385 pKa = 4.21 CGDD388 pKa = 3.7 LTLRR392 pKa = 11.84 LPRR395 pKa = 11.84 LNEE398 pKa = 3.66 LLQRR402 pKa = 11.84 LAVRR406 pKa = 11.84 KK407 pKa = 8.99 EE408 pKa = 3.63 VTADD412 pKa = 3.43 VVIDD416 pKa = 3.59 TVRR419 pKa = 11.84 RR420 pKa = 11.84 LAHH423 pKa = 5.31 EE424 pKa = 4.54 EE425 pKa = 3.73 KK426 pKa = 8.48 WTTEE430 pKa = 3.1 IDD432 pKa = 3.45 RR433 pKa = 11.84 DD434 pKa = 4.04 EE435 pKa = 4.72 FEE437 pKa = 4.48 LWLSRR442 pKa = 11.84 VVTEE446 pKa = 4.3 AGEE449 pKa = 4.09 RR450 pKa = 11.84 AQPLTLAYY458 pKa = 9.78 TNGQCWTCGTFGKK471 pKa = 7.08 THH473 pKa = 5.6 RR474 pKa = 11.84 HH475 pKa = 4.33 EE476 pKa = 4.95 CKK478 pKa = 9.4 ACKK481 pKa = 9.55 RR482 pKa = 11.84 RR483 pKa = 11.84 ARR485 pKa = 11.84 NRR487 pKa = 11.84 VPDD490 pKa = 4.11 RR491 pKa = 11.84 PLSDD495 pKa = 4.21 AFATHH500 pKa = 6.35 MGFFPLWSEE509 pKa = 4.3 RR510 pKa = 11.84 FMLPNMAFKK519 pKa = 10.7 RR520 pKa = 11.84 GTQVRR525 pKa = 11.84 VGKK528 pKa = 10.42 AGNRR532 pKa = 11.84 VMTTRR537 pKa = 11.84 AEE539 pKa = 4.09 VNAWLSRR546 pKa = 11.84 QRR548 pKa = 11.84 VDD550 pKa = 2.94 VSQRR554 pKa = 11.84 GRR556 pKa = 11.84 LCGPMFCNQVPKK568 pKa = 10.43 CYY570 pKa = 9.92 PKK572 pKa = 10.44 GQAVALSAFLVRR584 pKa = 11.84 LGGTRR589 pKa = 11.84 PYY591 pKa = 10.54 PDD593 pKa = 3.6 TFPCQMCASDD603 pKa = 3.58 GHH605 pKa = 5.7 EE606 pKa = 4.2 EE607 pKa = 4.23 CTKK610 pKa = 10.64 QCTPDD615 pKa = 3.47 EE616 pKa = 4.45 LVFAPNNVRR625 pKa = 11.84 PEE627 pKa = 3.79 VKK629 pKa = 10.07 LAYY632 pKa = 9.95 DD633 pKa = 3.66 LLYY636 pKa = 10.4 TVFSQYY642 pKa = 10.86 VLYY645 pKa = 10.92 GGLTSASCPLKK656 pKa = 10.53 PEE658 pKa = 3.84 SRR660 pKa = 11.84 TEE662 pKa = 4.01 FLSHH666 pKa = 7.04 FAGEE670 pKa = 4.46 KK671 pKa = 8.52 LLKK674 pKa = 9.71 MVEE677 pKa = 4.06 ALAMINDD684 pKa = 3.71 GWLEE688 pKa = 3.97 NMDD691 pKa = 4.21 DD692 pKa = 3.42 EE693 pKa = 4.61 AVRR696 pKa = 11.84 VRR698 pKa = 11.84 FTGFTKK704 pKa = 10.62 AEE706 pKa = 3.7 KK707 pKa = 10.49 SYY709 pKa = 11.01 DD710 pKa = 3.74 FEE712 pKa = 4.47 WTGDD716 pKa = 3.66 CLILKK721 pKa = 10.22 KK722 pKa = 10.28 EE723 pKa = 3.98 GKK725 pKa = 9.28 PRR727 pKa = 11.84 FICSPDD733 pKa = 3.4 PVMLYY738 pKa = 10.05 YY739 pKa = 10.86 LGRR742 pKa = 11.84 YY743 pKa = 5.89 THH745 pKa = 6.55 VQTKK749 pKa = 8.5 WLSARR754 pKa = 11.84 FLPCSRR760 pKa = 11.84 MFYY763 pKa = 10.22 AGCATPEE770 pKa = 4.48 DD771 pKa = 3.92 MNVWLNYY778 pKa = 8.27 TAGDD782 pKa = 3.73 AQYY785 pKa = 10.46 MNTIVDD791 pKa = 4.42 DD792 pKa = 4.29 VSAMDD797 pKa = 3.75 SSFTKK802 pKa = 10.24 TLLDD806 pKa = 3.3 FHH808 pKa = 6.69 EE809 pKa = 5.68 RR810 pKa = 11.84 IRR812 pKa = 11.84 DD813 pKa = 3.48 LQFPHH818 pKa = 6.31 MAAHH822 pKa = 6.41 TKK824 pKa = 9.84 AAFRR828 pKa = 11.84 GEE830 pKa = 3.58 EE831 pKa = 4.14 RR832 pKa = 11.84 FWVRR836 pKa = 11.84 IGEE839 pKa = 3.83 IRR841 pKa = 11.84 AFVEE845 pKa = 4.1 WVNASGVPDD854 pKa = 3.7 TSYY857 pKa = 11.76 KK858 pKa = 9.03 NTAPCLPLRR867 pKa = 11.84 VFAIVHH873 pKa = 6.78 AIWGMIGQPFEE884 pKa = 4.2 LTIEE888 pKa = 4.34 RR889 pKa = 11.84 YY890 pKa = 9.95 YY891 pKa = 11.31 LVLDD895 pKa = 4.86 GIYY898 pKa = 10.17 TSAAGDD904 pKa = 3.79 DD905 pKa = 3.85 GLTRR909 pKa = 11.84 VPTHH913 pKa = 5.89 MFGVDD918 pKa = 3.1 TSSPQFKK925 pKa = 9.69 MGYY928 pKa = 8.31 CKK930 pKa = 10.26 AWSFFGFNVKK940 pKa = 9.79 VDD942 pKa = 3.6 IVPPCRR948 pKa = 11.84 WRR950 pKa = 11.84 MATYY954 pKa = 10.06 LAQRR958 pKa = 11.84 PVWVGHH964 pKa = 5.75 RR965 pKa = 11.84 YY966 pKa = 8.37 EE967 pKa = 4.4 WAPEE971 pKa = 3.46 PARR974 pKa = 11.84 RR975 pKa = 11.84 LRR977 pKa = 11.84 GIFWQIDD984 pKa = 3.77 CSLHH988 pKa = 5.63 PIAWGRR994 pKa = 11.84 GIATQLLQQAKK1005 pKa = 8.68 ALPVVSHH1012 pKa = 5.55 VCQWYY1017 pKa = 9.05 LARR1020 pKa = 11.84 TSGPAIISAATAASHH1035 pKa = 6.57 EE1036 pKa = 4.18 YY1037 pKa = 10.78 SPFHH1041 pKa = 6.49 GAVCSGDD1048 pKa = 3.15 INEE1051 pKa = 4.66 RR1052 pKa = 11.84 GVSEE1056 pKa = 4.21 FCVDD1060 pKa = 3.43 YY1061 pKa = 10.93 NIPRR1065 pKa = 11.84 AEE1067 pKa = 4.15 LDD1069 pKa = 3.4 RR1070 pKa = 11.84 FCRR1073 pKa = 11.84 LLEE1076 pKa = 4.09 LVPSVLVNLNSFVLDD1091 pKa = 4.21 RR1092 pKa = 11.84 IYY1094 pKa = 11.24 AEE1096 pKa = 4.3 EE1097 pKa = 4.06 SS1098 pKa = 3.15
Molecular weight: 123.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.851
IPC2_protein 7.059
IPC_protein 6.883
Toseland 6.62
ProMoST 7.468
Dawson 7.541
Bjellqvist 8.024
Wikipedia 7.454
Rodwell 7.556
Grimsley 6.678
Solomon 7.556
Lehninger 7.585
Nozaki 8.302
DTASelect 7.746
Thurlkill 7.79
EMBOSS 7.79
Sillero 8.126
Patrickios 3.973
IPC_peptide 7.556
IPC2_peptide 7.439
IPC2.peptide.svr19 7.129
Protein with the highest isoelectric point:
>tr|A0A1L3KL89|A0A1L3KL89_9VIRU Uncharacterized protein OS=Shahe isopoda virus 3 OX=1923423 PE=4 SV=1
MM1 pKa = 7.73 PSSKK5 pKa = 10.11 PSQLDD10 pKa = 3.68 LVSLPTLCKK19 pKa = 10.03 TISLPGDD26 pKa = 3.69 YY27 pKa = 10.5 PPVRR31 pKa = 11.84 LPTYY35 pKa = 9.07 PAVEE39 pKa = 4.22 RR40 pKa = 11.84 TSVLPFNATLTTTVPSEE57 pKa = 4.07 GMRR60 pKa = 11.84 GMLIKK65 pKa = 10.67 SSTMPLWTDD74 pKa = 3.4 APVVAPYY81 pKa = 10.16 SYY83 pKa = 11.31 TMGWDD88 pKa = 3.29 ISPTVGTPAATSSIWPIMPDD108 pKa = 2.94 AKK110 pKa = 10.77 GAGGGSFSADD120 pKa = 2.73 VRR122 pKa = 11.84 NGLEE126 pKa = 4.46 VIGSNPPPGGYY137 pKa = 9.51 PIVGEE142 pKa = 4.88 LDD144 pKa = 3.33 LKK146 pKa = 11.22 AYY148 pKa = 9.76 IYY150 pKa = 10.06 LPKK153 pKa = 10.07 GARR156 pKa = 11.84 VLITAFGPSVPDD168 pKa = 3.11 SGANLTLEE176 pKa = 4.07 QAEE179 pKa = 4.34 LGGTIASEE187 pKa = 4.06 NYY189 pKa = 9.56 ALNVGAGATASYY201 pKa = 10.3 KK202 pKa = 10.54 GYY204 pKa = 9.99 FRR206 pKa = 11.84 EE207 pKa = 4.08 FTIPKK212 pKa = 9.89 SFFARR217 pKa = 11.84 PLSLSINRR225 pKa = 11.84 AWYY228 pKa = 9.83 GFTLNITVMNKK239 pKa = 9.05 VPIMNASTGNMTLTWSPTEE258 pKa = 3.7 PSIPLMVPAIKK269 pKa = 10.26 PPALDD274 pKa = 3.1 ISAIPFSNTRR284 pKa = 11.84 VTALAALFTNVTKK297 pKa = 10.83 ALNKK301 pKa = 9.89 EE302 pKa = 4.17 GTVMAGRR309 pKa = 11.84 FNPATLDD316 pKa = 3.86 ALDD319 pKa = 4.49 LTMADD324 pKa = 3.89 YY325 pKa = 11.03 SQLAPCEE332 pKa = 4.21 KK333 pKa = 10.58 YY334 pKa = 10.55 FFGLEE339 pKa = 3.94 KK340 pKa = 10.74 GFYY343 pKa = 9.81 TYY345 pKa = 10.61 IPLQTDD351 pKa = 3.12 VGEE354 pKa = 4.45 FADD357 pKa = 4.91 DD358 pKa = 3.28 VWDD361 pKa = 4.31 APQISVTGGVRR372 pKa = 11.84 IPWLHH377 pKa = 6.97 LGRR380 pKa = 11.84 TALVNTFRR388 pKa = 11.84 FDD390 pKa = 3.93 DD391 pKa = 4.52 PDD393 pKa = 5.34 GGTSLAVNLDD403 pKa = 2.98 WHH405 pKa = 5.8 VEE407 pKa = 3.94 YY408 pKa = 10.85 RR409 pKa = 11.84 NSSVLWPVAVSALSLEE425 pKa = 4.42 EE426 pKa = 3.61 AHH428 pKa = 7.32 RR429 pKa = 11.84 AQLVCLEE436 pKa = 4.56 AGFFFDD442 pKa = 5.4 NVDD445 pKa = 3.27 HH446 pKa = 6.98 KK447 pKa = 11.09 FILSMVSKK455 pKa = 10.13 GLGMVAPMLKK465 pKa = 9.97 NRR467 pKa = 11.84 APAIASAGMAARR479 pKa = 11.84 GYY481 pKa = 8.97 IRR483 pKa = 11.84 SYY485 pKa = 10.63 LGEE488 pKa = 4.23 SSHH491 pKa = 6.89 PKK493 pKa = 9.76 PSSLQVATAPRR504 pKa = 11.84 VKK506 pKa = 10.21 VRR508 pKa = 11.84 HH509 pKa = 6.0 PKK511 pKa = 10.1 DD512 pKa = 3.5 KK513 pKa = 10.29 KK514 pKa = 10.84 AKK516 pKa = 9.29 AAPRR520 pKa = 11.84 KK521 pKa = 9.35 PKK523 pKa = 10.1 SDD525 pKa = 3.03 KK526 pKa = 10.55 KK527 pKa = 10.65 KK528 pKa = 10.4 KK529 pKa = 10.19 KK530 pKa = 9.88 KK531 pKa = 10.33
Molecular weight: 57.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.656
IPC2_protein 8.624
IPC_protein 8.521
Toseland 9.165
ProMoST 9.063
Dawson 9.472
Bjellqvist 9.224
Wikipedia 9.663
Rodwell 9.692
Grimsley 9.56
Solomon 9.516
Lehninger 9.472
Nozaki 9.268
DTASelect 9.194
Thurlkill 9.311
EMBOSS 9.604
Sillero 9.428
Patrickios 4.66
IPC_peptide 9.516
IPC2_peptide 7.775
IPC2.peptide.svr19 7.722
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1629
531
1098
814.5
90.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.699 ± 0.342
2.394 ± 0.95
4.604 ± 0.044
4.85 ± 0.758
4.911 ± 0.497
6.446 ± 0.565
1.78 ± 0.338
3.683 ± 0.434
4.174 ± 0.864
9.208 ± 0.01
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.885 ± 0.066
3.376 ± 0.203
7.612 ± 0.839
2.578 ± 0.654
6.2 ± 1.166
5.893 ± 1.243
6.507 ± 0.435
7.551 ± 0.303
2.087 ± 0.204
3.56 ± 0.089
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here