Curvularia thermal tolerance virus
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A3EYA9|A3EYA9_9VIRU Uncharacterized protein OS=Curvularia thermal tolerance virus OX=421976 PE=4 SV=1
MM1 pKa = 7.56 AAMSAMTRR9 pKa = 11.84 TGTLFANLSWRR20 pKa = 11.84 LLSAGNDD27 pKa = 4.07 GIRR30 pKa = 11.84 LQKK33 pKa = 10.54 RR34 pKa = 11.84 VGTTSGHH41 pKa = 4.66 SHH43 pKa = 5.52 NTLLQSICTLIVGYY57 pKa = 9.81 GVLMATHH64 pKa = 7.72 PDD66 pKa = 3.43 LTDD69 pKa = 3.52 EE70 pKa = 4.81 EE71 pKa = 4.5 IKK73 pKa = 10.77 AAAQIEE79 pKa = 4.61 SLGDD83 pKa = 3.66 DD84 pKa = 4.36 NITGTKK90 pKa = 10.35 APLQPLPVEE99 pKa = 4.54 DD100 pKa = 4.35 VADD103 pKa = 4.14 KK104 pKa = 10.76 AWDD107 pKa = 3.4 MFGIDD112 pKa = 3.27 WSGKK116 pKa = 9.74 KK117 pKa = 10.14 SFATTAVLDD126 pKa = 3.72 ATALQFQGVQYY137 pKa = 10.03 LGKK140 pKa = 8.68 YY141 pKa = 8.16 FRR143 pKa = 11.84 RR144 pKa = 11.84 EE145 pKa = 3.96 EE146 pKa = 4.19 YY147 pKa = 10.1 PLDD150 pKa = 3.85 EE151 pKa = 4.49 GTVVVAIPYY160 pKa = 10.06 RR161 pKa = 11.84 PFKK164 pKa = 9.56 EE165 pKa = 4.21 TYY167 pKa = 10.02 LRR169 pKa = 11.84 LLYY172 pKa = 9.93 PEE174 pKa = 4.58 YY175 pKa = 11.08 GSLEE179 pKa = 4.07 ADD181 pKa = 3.66 QTWLRR186 pKa = 11.84 LLGNYY191 pKa = 9.51 LDD193 pKa = 4.5 AAGNPVTEE201 pKa = 4.21 KK202 pKa = 9.99 WLQGFMDD209 pKa = 3.77 WLEE212 pKa = 4.26 PQVVAPPQVWPSNFQRR228 pKa = 11.84 MVSRR232 pKa = 11.84 DD233 pKa = 3.32 YY234 pKa = 11.49 SGIGIEE240 pKa = 4.14 MPRR243 pKa = 11.84 PEE245 pKa = 4.29 RR246 pKa = 11.84 MCYY249 pKa = 7.86 EE250 pKa = 3.72 QWRR253 pKa = 11.84 DD254 pKa = 3.54 LVVLPRR260 pKa = 11.84 DD261 pKa = 4.68 DD262 pKa = 5.58 YY263 pKa = 10.67 RR264 pKa = 11.84 QLWKK268 pKa = 10.88 ASDD271 pKa = 4.06 PNDD274 pKa = 3.73 DD275 pKa = 3.92 ALYY278 pKa = 8.89 EE279 pKa = 4.03 AEE281 pKa = 4.31 YY282 pKa = 11.25
Molecular weight: 31.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.67
IPC2_protein 4.711
IPC_protein 4.647
Toseland 4.482
ProMoST 4.762
Dawson 4.609
Bjellqvist 4.749
Wikipedia 4.495
Rodwell 4.482
Grimsley 4.393
Solomon 4.609
Lehninger 4.558
Nozaki 4.711
DTASelect 4.902
Thurlkill 4.495
EMBOSS 4.507
Sillero 4.762
Patrickios 3.795
IPC_peptide 4.609
IPC2_peptide 4.762
IPC2.peptide.svr19 4.704
Protein with the highest isoelectric point:
>tr|A3EYB0|A3EYB0_9VIRU Uncharacterized protein OS=Curvularia thermal tolerance virus OX=421976 PE=4 SV=1
MM1 pKa = 8.14 SGMKK5 pKa = 10.37 APVSFLEE12 pKa = 4.39 KK13 pKa = 10.84 YY14 pKa = 10.55 GGATANVDD22 pKa = 4.1 FSEE25 pKa = 5.27 GPSLPAEE32 pKa = 4.37 VLPMPEE38 pKa = 4.58 PTDD41 pKa = 3.7 SLQTLSNKK49 pKa = 8.33 MRR51 pKa = 11.84 ALAEE55 pKa = 4.11 VVEE58 pKa = 5.12 RR59 pKa = 11.84 GASVARR65 pKa = 11.84 FTGMLGTPQGSRR77 pKa = 11.84 DD78 pKa = 3.53 AEE80 pKa = 4.11 EE81 pKa = 4.03 VGKK84 pKa = 10.63 LRR86 pKa = 11.84 MTDD89 pKa = 3.63 LEE91 pKa = 4.65 RR92 pKa = 11.84 EE93 pKa = 4.39 MFDD96 pKa = 2.56 HH97 pKa = 6.79 WAAGGVLPPIDD108 pKa = 3.82 WDD110 pKa = 3.54 KK111 pKa = 11.96 DD112 pKa = 3.49 MKK114 pKa = 10.48 KK115 pKa = 10.39 APSSGEE121 pKa = 3.66 GMRR124 pKa = 11.84 RR125 pKa = 11.84 AQRR128 pKa = 11.84 RR129 pKa = 11.84 AQALNMLYY137 pKa = 8.51 KK138 pKa = 9.79 TDD140 pKa = 3.66 KK141 pKa = 10.78 AQPQHH146 pKa = 6.06 VMWYY150 pKa = 8.04 QDD152 pKa = 3.13 TYY154 pKa = 11.0 ATIIPLLVAVTKK166 pKa = 10.79 VIGAQTDD173 pKa = 3.9 FLSGQDD179 pKa = 3.37 KK180 pKa = 9.55 LTDD183 pKa = 3.51 NEE185 pKa = 4.13 VAEE188 pKa = 4.32 VQTIGRR194 pKa = 11.84 ATGTTYY200 pKa = 10.4 ATINAIQEE208 pKa = 4.31 EE209 pKa = 4.86 VHH211 pKa = 6.92 KK212 pKa = 10.93 LLANINRR219 pKa = 11.84 SKK221 pKa = 11.16 DD222 pKa = 3.3 VLQARR227 pKa = 11.84 EE228 pKa = 3.86 HH229 pKa = 6.57 AIKK232 pKa = 10.6 AKK234 pKa = 10.19 NPEE237 pKa = 4.2 YY238 pKa = 10.24 KK239 pKa = 10.22 KK240 pKa = 10.71 KK241 pKa = 10.52 RR242 pKa = 11.84 SPEE245 pKa = 3.49 NDD247 pKa = 2.77 RR248 pKa = 11.84 RR249 pKa = 11.84 GLLGKK254 pKa = 10.2 RR255 pKa = 11.84 KK256 pKa = 9.73 IGVEE260 pKa = 3.84 VDD262 pKa = 4.33 FGQAPEE268 pKa = 3.82 PSRR271 pKa = 11.84 RR272 pKa = 11.84 ARR274 pKa = 11.84 RR275 pKa = 11.84 DD276 pKa = 2.9 LAQGIRR282 pKa = 11.84 PRR284 pKa = 11.84 SVIEE288 pKa = 3.77 AAKK291 pKa = 8.99 ATAEE295 pKa = 4.01 SRR297 pKa = 11.84 EE298 pKa = 4.26 KK299 pKa = 10.83 GKK301 pKa = 10.35 QPAKK305 pKa = 9.77 EE306 pKa = 4.23 GNGTPPTAGRR316 pKa = 11.84 RR317 pKa = 11.84 DD318 pKa = 3.21 GHH320 pKa = 5.83 FQLGGWQDD328 pKa = 3.3 TGVV331 pKa = 3.14
Molecular weight: 36.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.775
IPC2_protein 8.697
IPC_protein 8.639
Toseland 9.604
ProMoST 9.209
Dawson 9.794
Bjellqvist 9.399
Wikipedia 9.911
Rodwell 10.218
Grimsley 9.838
Solomon 9.823
Lehninger 9.809
Nozaki 9.56
DTASelect 9.399
Thurlkill 9.648
EMBOSS 9.999
Sillero 9.692
Patrickios 9.502
IPC_peptide 9.838
IPC2_peptide 7.746
IPC2.peptide.svr19 7.686
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1152
168
371
288.0
32.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.028 ± 0.689
0.521 ± 0.185
6.337 ± 0.534
7.726 ± 0.712
3.125 ± 0.518
7.118 ± 1.086
1.563 ± 0.171
3.472 ± 0.103
4.774 ± 0.926
9.375 ± 0.924
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.125 ± 0.089
2.778 ± 0.109
5.903 ± 0.232
4.253 ± 0.562
7.378 ± 0.529
5.729 ± 0.878
6.25 ± 0.18
5.816 ± 0.172
2.344 ± 0.513
3.385 ± 0.576
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here