Cannes 8 virus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Marseilleviridae; Marseillevirus; unclassified Marseillevirus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 483 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T2AXC7|T2AXC7_9VIRU Small membrane protein OS=Cannes 8 virus OX=1394979 GN=C8_123 PE=4 SV=1
MM1 pKa = 7.16SLVRR5 pKa = 11.84GFFKK9 pKa = 10.57DD10 pKa = 3.24IPIVGSYY17 pKa = 10.72SVLPPVLPRR26 pKa = 11.84GSLVFLTTDD35 pKa = 2.98QNLYY39 pKa = 10.15ISNGQIWAVAGGDD52 pKa = 3.84VGPLAAQVAQNTADD66 pKa = 3.38IATLQTDD73 pKa = 3.63VTTLDD78 pKa = 3.56GQVATNTSNITALQGQVATNTSDD101 pKa = 2.77IGTLQGQVATNTTDD115 pKa = 2.52ITTLQGQVTTNTSNITTLQGQVATNTSDD143 pKa = 2.76ITSNTNSINNIIVSQSQGFTIADD166 pKa = 3.64SAQLRR171 pKa = 11.84TFTNPITRR179 pKa = 11.84IDD181 pKa = 3.71VSSTSPTVIYY191 pKa = 10.0TLGTPLDD198 pKa = 3.85GSFNGVYY205 pKa = 10.54LIQWNILAHH214 pKa = 5.22YY215 pKa = 7.21QTANVFYY222 pKa = 10.62AVGQSSVLSNGIGALVSVFGTSTNQTNSSGSSVITVTADD261 pKa = 2.85INLNNYY267 pKa = 9.3RR268 pKa = 11.84LIVTSDD274 pKa = 3.3SAVTTTYY281 pKa = 10.17SGYY284 pKa = 10.92VIVTRR289 pKa = 11.84ILTISPP295 pKa = 3.78

Molecular weight: 30.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T2AXZ1|T2AXZ1_9VIRU Uncharacterized protein OS=Cannes 8 virus OX=1394979 GN=C8_184 PE=4 SV=1
MM1 pKa = 7.35SRR3 pKa = 11.84AKK5 pKa = 10.53KK6 pKa = 8.25ITEE9 pKa = 4.09PFLVAWTLLGIPAGFVIGRR28 pKa = 11.84GEE30 pKa = 4.21GRR32 pKa = 11.84NQKK35 pKa = 9.01NRR37 pKa = 11.84HH38 pKa = 4.42VSWYY42 pKa = 8.82VGVPKK47 pKa = 10.51VVWRR51 pKa = 11.84TICWPYY57 pKa = 10.57YY58 pKa = 7.69VRR60 pKa = 11.84EE61 pKa = 4.18YY62 pKa = 10.82II63 pKa = 4.45

Molecular weight: 7.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

483

0

483

113970

44

1537

236.0

27.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.097 ± 0.12

2.594 ± 0.095

4.581 ± 0.075

8.917 ± 0.169

5.882 ± 0.094

6.248 ± 0.109

1.873 ± 0.056

4.849 ± 0.077

8.916 ± 0.189

8.845 ± 0.142

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.029 ± 0.05

3.853 ± 0.083

3.924 ± 0.083

3.31 ± 0.077

5.686 ± 0.1

7.708 ± 0.115

4.835 ± 0.104

6.294 ± 0.088

1.597 ± 0.051

2.964 ± 0.066
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski