Amino acid dipepetide frequency for Lactococcus phage CHPC362

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.485AlaAla: 0.485 ± 0.203
0.121AlaCys: 0.121 ± 0.111
3.029AlaAsp: 3.029 ± 0.626
4.604AlaGlu: 4.604 ± 0.941
3.393AlaPhe: 3.393 ± 0.748
4.726AlaGly: 4.726 ± 1.151
0.848AlaHis: 0.848 ± 0.34
4.847AlaIle: 4.847 ± 1.026
5.574AlaLys: 5.574 ± 0.864
6.543AlaLeu: 6.543 ± 1.129
2.787AlaMet: 2.787 ± 0.649
3.999AlaAsn: 3.999 ± 0.927
0.727AlaPro: 0.727 ± 0.292
2.302AlaGln: 2.302 ± 0.69
1.939AlaArg: 1.939 ± 0.426
3.514AlaSer: 3.514 ± 0.867
3.393AlaThr: 3.393 ± 0.908
4.604AlaVal: 4.604 ± 1.295
1.696AlaTrp: 1.696 ± 0.811
1.696AlaTyr: 1.696 ± 0.457
0.0AlaXaa: 0.0 ± 0.0
Cys
0.121CysAla: 0.121 ± 0.13
0.0CysCys: 0.0 ± 0.0
0.242CysAsp: 0.242 ± 0.17
0.242CysGlu: 0.242 ± 0.158
0.121CysPhe: 0.121 ± 0.127
0.848CysGly: 0.848 ± 0.319
0.242CysHis: 0.242 ± 0.205
0.606CysIle: 0.606 ± 0.263
0.485CysLys: 0.485 ± 0.268
0.485CysLeu: 0.485 ± 0.254
0.121CysMet: 0.121 ± 0.108
0.242CysAsn: 0.242 ± 0.177
0.242CysPro: 0.242 ± 0.203
0.364CysGln: 0.364 ± 0.215
0.727CysArg: 0.727 ± 0.283
0.364CysSer: 0.364 ± 0.203
0.121CysThr: 0.121 ± 0.11
0.242CysVal: 0.242 ± 0.161
0.121CysTrp: 0.121 ± 0.127
0.364CysTyr: 0.364 ± 0.199
0.0CysXaa: 0.0 ± 0.0
Asp
1.575AspAla: 1.575 ± 0.456
0.121AspCys: 0.121 ± 0.115
3.756AspAsp: 3.756 ± 0.876
3.999AspGlu: 3.999 ± 0.988
3.272AspPhe: 3.272 ± 0.636
4.241AspGly: 4.241 ± 0.695
0.969AspHis: 0.969 ± 0.423
3.999AspIle: 3.999 ± 0.806
4.968AspLys: 4.968 ± 0.57
6.301AspLeu: 6.301 ± 1.047
1.091AspMet: 1.091 ± 0.354
4.483AspAsn: 4.483 ± 0.703
1.454AspPro: 1.454 ± 0.504
0.848AspGln: 0.848 ± 0.348
1.696AspArg: 1.696 ± 0.621
2.908AspSer: 2.908 ± 0.588
3.999AspThr: 3.999 ± 0.699
3.272AspVal: 3.272 ± 0.604
0.606AspTrp: 0.606 ± 0.209
2.666AspTyr: 2.666 ± 0.531
0.0AspXaa: 0.0 ± 0.0
Glu
3.393GluAla: 3.393 ± 0.536
0.364GluCys: 0.364 ± 0.218
3.15GluAsp: 3.15 ± 0.744
3.514GluGlu: 3.514 ± 0.736
3.272GluPhe: 3.272 ± 0.69
1.939GluGly: 1.939 ± 0.474
0.606GluHis: 0.606 ± 0.369
5.331GluIle: 5.331 ± 0.662
4.968GluLys: 4.968 ± 1.011
9.936GluLeu: 9.936 ± 1.699
2.423GluMet: 2.423 ± 0.423
4.483GluAsn: 4.483 ± 0.692
1.091GluPro: 1.091 ± 0.363
3.635GluGln: 3.635 ± 0.83
2.545GluArg: 2.545 ± 0.619
4.12GluSer: 4.12 ± 0.458
4.604GluThr: 4.604 ± 0.761
4.12GluVal: 4.12 ± 0.648
0.848GluTrp: 0.848 ± 0.302
3.029GluTyr: 3.029 ± 0.825
0.0GluXaa: 0.0 ± 0.0
Phe
3.15PheAla: 3.15 ± 0.85
0.242PheCys: 0.242 ± 0.16
3.029PheAsp: 3.029 ± 0.587
2.423PheGlu: 2.423 ± 0.677
2.06PhePhe: 2.06 ± 0.85
1.939PheGly: 1.939 ± 0.547
0.242PheHis: 0.242 ± 0.177
3.393PheIle: 3.393 ± 0.551
3.514PheLys: 3.514 ± 0.732
2.423PheLeu: 2.423 ± 0.623
0.969PheMet: 0.969 ± 0.326
2.908PheAsn: 2.908 ± 0.806
0.969PhePro: 0.969 ± 0.355
1.333PheGln: 1.333 ± 0.443
1.333PheArg: 1.333 ± 0.335
4.726PheSer: 4.726 ± 1.313
3.15PheThr: 3.15 ± 0.6
2.302PheVal: 2.302 ± 0.447
0.242PheTrp: 0.242 ± 0.165
1.696PheTyr: 1.696 ± 0.43
0.0PheXaa: 0.0 ± 0.0
Gly
3.877GlyAla: 3.877 ± 1.428
0.485GlyCys: 0.485 ± 0.245
3.272GlyAsp: 3.272 ± 0.811
3.877GlyGlu: 3.877 ± 0.703
2.666GlyPhe: 2.666 ± 0.653
4.604GlyGly: 4.604 ± 1.01
0.606GlyHis: 0.606 ± 0.251
5.089GlyIle: 5.089 ± 1.76
6.664GlyLys: 6.664 ± 0.703
5.816GlyLeu: 5.816 ± 1.474
1.091GlyMet: 1.091 ± 0.397
3.393GlyAsn: 3.393 ± 0.584
0.121GlyPro: 0.121 ± 0.118
2.302GlyGln: 2.302 ± 0.588
1.454GlyArg: 1.454 ± 0.336
5.695GlySer: 5.695 ± 1.318
3.514GlyThr: 3.514 ± 0.985
7.028GlyVal: 7.028 ± 1.602
1.333GlyTrp: 1.333 ± 0.382
2.908GlyTyr: 2.908 ± 0.673
0.0GlyXaa: 0.0 ± 0.0
His
0.848HisAla: 0.848 ± 0.289
0.727HisCys: 0.727 ± 0.365
0.727HisAsp: 0.727 ± 0.365
0.606HisGlu: 0.606 ± 0.228
0.242HisPhe: 0.242 ± 0.175
1.454HisGly: 1.454 ± 0.556
0.0HisHis: 0.0 ± 0.0
0.848HisIle: 0.848 ± 0.262
0.727HisLys: 0.727 ± 0.282
0.606HisLeu: 0.606 ± 0.272
0.0HisMet: 0.0 ± 0.0
1.454HisAsn: 1.454 ± 0.483
0.242HisPro: 0.242 ± 0.147
0.242HisGln: 0.242 ± 0.187
0.242HisArg: 0.242 ± 0.174
0.485HisSer: 0.485 ± 0.244
0.969HisThr: 0.969 ± 0.385
0.969HisVal: 0.969 ± 0.461
0.0HisTrp: 0.0 ± 0.0
0.485HisTyr: 0.485 ± 0.28
0.0HisXaa: 0.0 ± 0.0
Ile
4.847IleAla: 4.847 ± 0.744
0.121IleCys: 0.121 ± 0.122
4.604IleAsp: 4.604 ± 0.638
5.21IleGlu: 5.21 ± 1.116
2.908IlePhe: 2.908 ± 0.592
3.999IleGly: 3.999 ± 1.015
1.091IleHis: 1.091 ± 0.368
5.331IleIle: 5.331 ± 0.778
6.785IleLys: 6.785 ± 1.012
5.453IleLeu: 5.453 ± 1.031
1.818IleMet: 1.818 ± 0.567
5.574IleAsn: 5.574 ± 0.603
1.939IlePro: 1.939 ± 0.547
2.06IleGln: 2.06 ± 0.367
1.696IleArg: 1.696 ± 0.569
4.12IleSer: 4.12 ± 0.996
5.21IleThr: 5.21 ± 0.837
3.999IleVal: 3.999 ± 0.661
1.212IleTrp: 1.212 ± 0.528
2.423IleTyr: 2.423 ± 0.495
0.0IleXaa: 0.0 ± 0.0
Lys
5.816LysAla: 5.816 ± 1.088
0.485LysCys: 0.485 ± 0.259
4.847LysAsp: 4.847 ± 0.794
7.512LysGlu: 7.512 ± 1.356
2.302LysPhe: 2.302 ± 0.547
5.453LysGly: 5.453 ± 0.948
1.212LysHis: 1.212 ± 0.413
4.726LysIle: 4.726 ± 0.857
7.755LysLys: 7.755 ± 1.132
6.543LysLeu: 6.543 ± 0.988
2.908LysMet: 2.908 ± 0.504
5.21LysAsn: 5.21 ± 0.823
1.939LysPro: 1.939 ± 0.572
3.514LysGln: 3.514 ± 0.73
2.908LysArg: 2.908 ± 0.57
5.695LysSer: 5.695 ± 0.923
5.21LysThr: 5.21 ± 1.158
5.816LysVal: 5.816 ± 0.911
0.969LysTrp: 0.969 ± 0.312
3.272LysTyr: 3.272 ± 0.705
0.0LysXaa: 0.0 ± 0.0
Leu
5.331LeuAla: 5.331 ± 0.568
0.242LeuCys: 0.242 ± 0.174
4.847LeuAsp: 4.847 ± 0.672
5.089LeuGlu: 5.089 ± 0.89
3.272LeuPhe: 3.272 ± 0.713
4.604LeuGly: 4.604 ± 1.05
1.212LeuHis: 1.212 ± 0.404
7.634LeuIle: 7.634 ± 1.101
8.361LeuLys: 8.361 ± 1.158
6.785LeuLeu: 6.785 ± 1.31
2.06LeuMet: 2.06 ± 0.568
5.21LeuAsn: 5.21 ± 1.029
3.272LeuPro: 3.272 ± 0.596
3.635LeuGln: 3.635 ± 0.537
2.666LeuArg: 2.666 ± 0.633
5.21LeuSer: 5.21 ± 0.849
6.301LeuThr: 6.301 ± 0.694
6.301LeuVal: 6.301 ± 0.63
1.575LeuTrp: 1.575 ± 0.474
4.362LeuTyr: 4.362 ± 0.921
0.0LeuXaa: 0.0 ± 0.0
Met
2.423MetAla: 2.423 ± 0.527
0.121MetCys: 0.121 ± 0.122
1.212MetAsp: 1.212 ± 0.352
1.454MetGlu: 1.454 ± 0.479
0.727MetPhe: 0.727 ± 0.356
1.091MetGly: 1.091 ± 0.384
0.242MetHis: 0.242 ± 0.176
2.181MetIle: 2.181 ± 0.535
2.666MetLys: 2.666 ± 0.555
1.212MetLeu: 1.212 ± 0.416
0.364MetMet: 0.364 ± 0.218
2.181MetAsn: 2.181 ± 0.598
0.606MetPro: 0.606 ± 0.31
1.818MetGln: 1.818 ± 0.37
0.485MetArg: 0.485 ± 0.307
2.06MetSer: 2.06 ± 0.379
1.575MetThr: 1.575 ± 0.452
1.333MetVal: 1.333 ± 0.368
0.485MetTrp: 0.485 ± 0.226
0.969MetTyr: 0.969 ± 0.403
0.0MetXaa: 0.0 ± 0.0
Asn
5.453AsnAla: 5.453 ± 1.073
0.242AsnCys: 0.242 ± 0.175
3.756AsnAsp: 3.756 ± 0.815
4.726AsnGlu: 4.726 ± 0.944
1.939AsnPhe: 1.939 ± 0.464
5.937AsnGly: 5.937 ± 0.918
0.727AsnHis: 0.727 ± 0.271
3.877AsnIle: 3.877 ± 0.719
5.453AsnLys: 5.453 ± 1.177
6.422AsnLeu: 6.422 ± 0.921
0.969AsnMet: 0.969 ± 0.376
3.635AsnAsn: 3.635 ± 0.769
1.696AsnPro: 1.696 ± 0.45
2.545AsnGln: 2.545 ± 0.474
2.181AsnArg: 2.181 ± 0.522
5.816AsnSer: 5.816 ± 0.816
4.847AsnThr: 4.847 ± 0.711
4.12AsnVal: 4.12 ± 0.553
1.212AsnTrp: 1.212 ± 0.422
2.423AsnTyr: 2.423 ± 0.742
0.0AsnXaa: 0.0 ± 0.0
Pro
1.696ProAla: 1.696 ± 0.456
0.121ProCys: 0.121 ± 0.12
1.575ProAsp: 1.575 ± 0.381
1.575ProGlu: 1.575 ± 0.424
0.848ProPhe: 0.848 ± 0.278
0.242ProGly: 0.242 ± 0.151
0.121ProHis: 0.121 ± 0.118
1.818ProIle: 1.818 ± 0.55
2.423ProLys: 2.423 ± 0.596
2.06ProLeu: 2.06 ± 0.431
0.606ProMet: 0.606 ± 0.261
2.181ProAsn: 2.181 ± 0.817
0.727ProPro: 0.727 ± 0.317
0.848ProGln: 0.848 ± 0.403
0.727ProArg: 0.727 ± 0.286
1.333ProSer: 1.333 ± 0.4
2.666ProThr: 2.666 ± 0.472
1.333ProVal: 1.333 ± 0.483
0.485ProTrp: 0.485 ± 0.247
0.727ProTyr: 0.727 ± 0.243
0.0ProXaa: 0.0 ± 0.0
Gln
3.15GlnAla: 3.15 ± 0.688
0.0GlnCys: 0.0 ± 0.0
1.818GlnAsp: 1.818 ± 0.588
2.181GlnGlu: 2.181 ± 0.598
1.212GlnPhe: 1.212 ± 0.369
2.666GlnGly: 2.666 ± 0.647
0.242GlnHis: 0.242 ± 0.158
1.333GlnIle: 1.333 ± 0.308
2.545GlnLys: 2.545 ± 0.692
3.514GlnLeu: 3.514 ± 0.834
1.091GlnMet: 1.091 ± 0.315
2.302GlnAsn: 2.302 ± 0.387
1.454GlnPro: 1.454 ± 0.397
1.454GlnGln: 1.454 ± 0.469
1.575GlnArg: 1.575 ± 0.381
2.545GlnSer: 2.545 ± 0.497
2.908GlnThr: 2.908 ± 0.438
2.545GlnVal: 2.545 ± 0.653
0.727GlnTrp: 0.727 ± 0.289
1.454GlnTyr: 1.454 ± 0.399
0.0GlnXaa: 0.0 ± 0.0
Arg
2.06ArgAla: 2.06 ± 0.619
0.485ArgCys: 0.485 ± 0.268
1.454ArgAsp: 1.454 ± 0.437
2.423ArgGlu: 2.423 ± 0.622
0.727ArgPhe: 0.727 ± 0.29
2.302ArgGly: 2.302 ± 0.547
0.727ArgHis: 0.727 ± 0.351
1.939ArgIle: 1.939 ± 0.482
3.635ArgLys: 3.635 ± 0.789
3.393ArgLeu: 3.393 ± 0.674
0.485ArgMet: 0.485 ± 0.267
2.423ArgAsn: 2.423 ± 0.55
0.485ArgPro: 0.485 ± 0.246
1.212ArgGln: 1.212 ± 0.393
1.575ArgArg: 1.575 ± 0.426
2.181ArgSer: 2.181 ± 0.587
1.696ArgThr: 1.696 ± 0.414
1.454ArgVal: 1.454 ± 0.538
0.364ArgTrp: 0.364 ± 0.219
1.575ArgTyr: 1.575 ± 0.461
0.0ArgXaa: 0.0 ± 0.0
Ser
5.331SerAla: 5.331 ± 1.462
0.606SerCys: 0.606 ± 0.337
4.12SerAsp: 4.12 ± 0.746
3.15SerGlu: 3.15 ± 0.605
3.272SerPhe: 3.272 ± 1.046
6.664SerGly: 6.664 ± 1.859
0.969SerHis: 0.969 ± 0.313
4.483SerIle: 4.483 ± 0.665
4.483SerLys: 4.483 ± 0.688
6.18SerLeu: 6.18 ± 0.913
2.181SerMet: 2.181 ± 0.455
4.483SerAsn: 4.483 ± 1.031
1.818SerPro: 1.818 ± 0.48
1.818SerGln: 1.818 ± 0.457
2.423SerArg: 2.423 ± 0.339
6.18SerSer: 6.18 ± 1.588
3.999SerThr: 3.999 ± 0.933
4.483SerVal: 4.483 ± 0.741
0.969SerTrp: 0.969 ± 0.396
2.423SerTyr: 2.423 ± 0.583
0.0SerXaa: 0.0 ± 0.0
Thr
5.21ThrAla: 5.21 ± 0.877
0.242ThrCys: 0.242 ± 0.178
2.787ThrAsp: 2.787 ± 0.644
5.574ThrGlu: 5.574 ± 0.808
2.908ThrPhe: 2.908 ± 0.676
5.331ThrGly: 5.331 ± 0.994
0.364ThrHis: 0.364 ± 0.231
4.12ThrIle: 4.12 ± 0.887
4.726ThrLys: 4.726 ± 0.588
6.18ThrLeu: 6.18 ± 0.864
1.333ThrMet: 1.333 ± 0.37
5.21ThrAsn: 5.21 ± 0.904
2.302ThrPro: 2.302 ± 0.336
2.181ThrGln: 2.181 ± 0.429
1.818ThrArg: 1.818 ± 0.518
4.604ThrSer: 4.604 ± 0.882
4.847ThrThr: 4.847 ± 0.739
5.21ThrVal: 5.21 ± 1.026
1.212ThrTrp: 1.212 ± 0.483
2.302ThrTyr: 2.302 ± 0.582
0.0ThrXaa: 0.0 ± 0.0
Val
3.635ValAla: 3.635 ± 0.549
0.606ValCys: 0.606 ± 0.315
4.847ValAsp: 4.847 ± 0.728
5.089ValGlu: 5.089 ± 0.639
3.393ValPhe: 3.393 ± 0.826
3.756ValGly: 3.756 ± 0.533
0.364ValHis: 0.364 ± 0.209
4.362ValIle: 4.362 ± 0.919
5.331ValLys: 5.331 ± 0.868
3.756ValLeu: 3.756 ± 0.613
1.818ValMet: 1.818 ± 0.509
3.514ValAsn: 3.514 ± 0.79
1.818ValPro: 1.818 ± 0.483
2.06ValGln: 2.06 ± 0.483
3.029ValArg: 3.029 ± 0.756
5.574ValSer: 5.574 ± 1.285
5.937ValThr: 5.937 ± 0.898
3.635ValVal: 3.635 ± 1.016
0.606ValTrp: 0.606 ± 0.24
2.908ValTyr: 2.908 ± 0.781
0.0ValXaa: 0.0 ± 0.0
Trp
0.848TrpAla: 0.848 ± 0.31
0.242TrpCys: 0.242 ± 0.164
0.848TrpAsp: 0.848 ± 0.369
0.727TrpGlu: 0.727 ± 0.303
1.091TrpPhe: 1.091 ± 0.427
1.212TrpGly: 1.212 ± 0.562
0.364TrpHis: 0.364 ± 0.286
0.848TrpIle: 0.848 ± 0.384
0.727TrpLys: 0.727 ± 0.229
1.212TrpLeu: 1.212 ± 0.363
0.364TrpMet: 0.364 ± 0.211
1.696TrpAsn: 1.696 ± 0.554
0.121TrpPro: 0.121 ± 0.118
0.969TrpGln: 0.969 ± 0.307
0.364TrpArg: 0.364 ± 0.335
1.212TrpSer: 1.212 ± 0.295
0.485TrpThr: 0.485 ± 0.283
0.606TrpVal: 0.606 ± 0.264
0.121TrpTrp: 0.121 ± 0.128
1.091TrpTyr: 1.091 ± 0.323
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.575TyrAla: 1.575 ± 0.522
0.727TyrCys: 0.727 ± 0.393
2.302TyrAsp: 2.302 ± 0.784
3.393TyrGlu: 3.393 ± 0.776
2.423TyrPhe: 2.423 ± 0.568
2.787TyrGly: 2.787 ± 0.551
0.727TyrHis: 0.727 ± 0.272
3.635TyrIle: 3.635 ± 0.925
2.423TyrLys: 2.423 ± 0.629
3.15TyrLeu: 3.15 ± 0.757
0.606TyrMet: 0.606 ± 0.297
3.393TyrAsn: 3.393 ± 0.603
1.091TyrPro: 1.091 ± 0.383
1.575TyrGln: 1.575 ± 0.557
1.212TyrArg: 1.212 ± 0.406
1.696TyrSer: 1.696 ± 0.434
3.029TyrThr: 3.029 ± 0.726
2.666TyrVal: 2.666 ± 0.574
0.364TyrTrp: 0.364 ± 0.176
2.06TyrTyr: 2.06 ± 0.557
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 45 proteins (8254 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski