Gordonia phage EMoore

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VU65|A0A649VU65_9CAUD Uncharacterized protein OS=Gordonia phage EMoore OX=2656534 GN=15 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 9.67RR3 pKa = 11.84TITAAAALLGALIAAVAIAAAPAQAVTTDD32 pKa = 2.81QYY34 pKa = 11.99DD35 pKa = 3.61NVRR38 pKa = 11.84LMMCGQGLVEE48 pKa = 5.63LEE50 pKa = 4.35FTNSTGGSSQGIVDD64 pKa = 4.37LDD66 pKa = 4.15EE67 pKa = 4.4YY68 pKa = 11.56TNGCYY73 pKa = 10.04FYY75 pKa = 11.12DD76 pKa = 3.39STEE79 pKa = 3.71YY80 pKa = 10.83DD81 pKa = 3.23EE82 pKa = 4.76YY83 pKa = 11.62GGYY86 pKa = 8.02VWSMITDD93 pKa = 3.95DD94 pKa = 5.08DD95 pKa = 4.57GGPVKK100 pKa = 10.48CAIWVNGALVSHH112 pKa = 6.75SNDD115 pKa = 3.03DD116 pKa = 3.4SPYY119 pKa = 9.76YY120 pKa = 10.99SFATCYY126 pKa = 10.57

Molecular weight: 13.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VTC8|A0A649VTC8_9CAUD Helix-turn-helix DNA binding protein OS=Gordonia phage EMoore OX=2656534 GN=1 PE=4 SV=1
MM1 pKa = 7.17MAACSSCGEE10 pKa = 4.12RR11 pKa = 11.84IVFGITANGKK21 pKa = 9.23NMPLDD26 pKa = 3.5EE27 pKa = 5.29HH28 pKa = 7.08PNLTKK33 pKa = 10.99GNVRR37 pKa = 11.84VLRR40 pKa = 11.84RR41 pKa = 11.84EE42 pKa = 4.07GPKK45 pKa = 9.61MYY47 pKa = 10.2VQSLGALAAAVARR60 pKa = 11.84TNGEE64 pKa = 4.02HH65 pKa = 7.08LYY67 pKa = 10.81LSHH70 pKa = 7.42FVNCPARR77 pKa = 11.84DD78 pKa = 3.3RR79 pKa = 11.84HH80 pKa = 5.6RR81 pKa = 11.84KK82 pKa = 7.88SRR84 pKa = 11.84RR85 pKa = 3.24

Molecular weight: 9.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

18787

48

1714

229.1

24.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.53 ± 0.52

0.931 ± 0.138

7.127 ± 0.413

5.44 ± 0.439

2.315 ± 0.176

8.655 ± 0.617

2.161 ± 0.219

4.115 ± 0.197

2.587 ± 0.183

7.878 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.225 ± 0.111

2.986 ± 0.165

6.393 ± 0.24

3.763 ± 0.198

7.186 ± 0.461

4.695 ± 0.268

7.095 ± 0.283

7.638 ± 0.224

2.071 ± 0.135

2.209 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski