Burkholderia virus Bcepil02

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lessievirus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5IHP1|C5IHP1_9CAUD CsrA-like transcriptional regulator OS=Burkholderia virus Bcepil02 OX=644524 GN=BcepIL02_gp49 PE=4 SV=1
MM1 pKa = 7.5GNYY4 pKa = 7.88TFSITAASKK13 pKa = 11.07ADD15 pKa = 3.51AKK17 pKa = 10.01TAIAAEE23 pKa = 4.51FDD25 pKa = 3.95RR26 pKa = 11.84KK27 pKa = 10.5VGADD31 pKa = 3.4PVVSRR36 pKa = 11.84ARR38 pKa = 11.84AAVVANAGAVIDD50 pKa = 5.48LLGDD54 pKa = 3.88DD55 pKa = 5.4DD56 pKa = 4.52NADD59 pKa = 3.44VTVQCSVFNYY69 pKa = 9.15TADD72 pKa = 4.0DD73 pKa = 4.42GEE75 pKa = 4.72GGQIARR81 pKa = 11.84SGGVSAFANHH91 pKa = 5.55VARR94 pKa = 11.84PVVVLTEE101 pKa = 4.25ADD103 pKa = 3.59SLADD107 pKa = 3.74LNGTPRR113 pKa = 11.84PDD115 pKa = 3.19NPTAA119 pKa = 3.82

Molecular weight: 12.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5IHM8|C5IHM8_9CAUD Uncharacterized protein OS=Burkholderia virus Bcepil02 OX=644524 GN=BcepIL02_gp36 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.42AMSILASIIGASRR15 pKa = 11.84RR16 pKa = 11.84FFTPDD21 pKa = 2.59QSFAIQRR28 pKa = 11.84IKK30 pKa = 10.78APRR33 pKa = 11.84PLRR36 pKa = 11.84RR37 pKa = 11.84STTAGVKK44 pKa = 9.86RR45 pKa = 11.84AATKK49 pKa = 9.75ARR51 pKa = 11.84NVKK54 pKa = 8.73RR55 pKa = 11.84HH56 pKa = 5.0RR57 pKa = 11.84AAMRR61 pKa = 11.84RR62 pKa = 11.84ARR64 pKa = 11.84AA65 pKa = 3.28

Molecular weight: 7.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

19839

41

4667

261.0

28.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.939 ± 0.529

0.796 ± 0.207

6.402 ± 0.172

6.427 ± 0.304

3.196 ± 0.167

8.216 ± 0.326

1.915 ± 0.231

4.269 ± 0.241

4.481 ± 0.316

7.45 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.515 ± 0.126

3.503 ± 0.18

5.545 ± 0.219

4.158 ± 0.321

7.631 ± 0.418

5.091 ± 0.198

5.388 ± 0.196

6.356 ± 0.218

1.356 ± 0.123

2.364 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski