Thermodesulfobacterium commune DSM 2178

Taxonomy: cellular organisms; Bacteria; Thermodesulfobacteria; Thermodesulfobacteria; Thermodesulfobacteriales; Thermodesulfobacteriaceae; Thermodesulfobacterium; Thermodesulfobacterium commune

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1430 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075WSS3|A0A075WSS3_9BACT Thiamine_BP domain-containing protein OS=Thermodesulfobacterium commune DSM 2178 OX=289377 GN=HL41_03495 PE=3 SV=1
MM1 pKa = 7.34KK2 pKa = 10.51VIFDD6 pKa = 4.06PDD8 pKa = 3.06IPEE11 pKa = 4.08EE12 pKa = 3.95LKK14 pKa = 10.91EE15 pKa = 4.69DD16 pKa = 3.63IIKK19 pKa = 10.68SIEE22 pKa = 3.95EE23 pKa = 4.06EE24 pKa = 4.17NVGEE28 pKa = 4.03ICKK31 pKa = 10.37VCGGDD36 pKa = 3.43TLYY39 pKa = 11.16VALLEE44 pKa = 4.41GVLDD48 pKa = 4.01VKK50 pKa = 10.99CYY52 pKa = 10.46EE53 pKa = 4.77CGHH56 pKa = 6.18SYY58 pKa = 10.65IEE60 pKa = 4.62LEE62 pKa = 4.26LAEE65 pKa = 4.34EE66 pKa = 4.32

Molecular weight: 7.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075WRX6|A0A075WRX6_9BACT Ribonuclease PH OS=Thermodesulfobacterium commune DSM 2178 OX=289377 GN=rph PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.22QPSRR9 pKa = 11.84LKK11 pKa = 9.72MKK13 pKa = 9.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.98GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.32TRR25 pKa = 11.84SGRR28 pKa = 11.84RR29 pKa = 11.84ILKK32 pKa = 8.64NRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.71GRR39 pKa = 11.84WRR41 pKa = 11.84LTVV44 pKa = 3.0

Molecular weight: 5.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1430

0

1430

451505

37

1742

315.7

35.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.965 ± 0.065

1.106 ± 0.028

4.167 ± 0.037

8.597 ± 0.083

5.463 ± 0.069

6.54 ± 0.064

1.542 ± 0.026

7.717 ± 0.059

9.182 ± 0.061

11.546 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.749 ± 0.027

3.612 ± 0.043

4.379 ± 0.042

2.886 ± 0.032

4.118 ± 0.043

5.048 ± 0.04

4.491 ± 0.046

7.1 ± 0.049

1.03 ± 0.024

3.761 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski