Hirsutella minnesotensis 3608

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Ophiocordycipitaceae; Hirsutella; Hirsutella minnesotensis

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9072 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F7ZKY1|A0A0F7ZKY1_9HYPO Uncharacterized protein OS=Hirsutella minnesotensis 3608 OX=1043627 GN=HIM_04918 PE=4 SV=1
MM1 pKa = 7.6SNPDD5 pKa = 3.23LSMSAFSGSIAQDD18 pKa = 2.97DD19 pKa = 4.11VMRR22 pKa = 11.84FSSQAQEE29 pKa = 4.2GTASLSGDD37 pKa = 3.42LRR39 pKa = 11.84STRR42 pKa = 11.84CQILSPSCRR51 pKa = 11.84LIASAHH57 pKa = 4.4QSGGRR62 pKa = 11.84EE63 pKa = 3.95NHH65 pKa = 6.05SPPLPSTPEE74 pKa = 3.67PRR76 pKa = 11.84NVYY79 pKa = 9.34HH80 pKa = 6.32QPQHH84 pKa = 3.6THH86 pKa = 5.18YY87 pKa = 10.34RR88 pKa = 11.84PFDD91 pKa = 3.76APEE94 pKa = 3.99PSFDD98 pKa = 5.11DD99 pKa = 3.08SDD101 pKa = 4.35YY102 pKa = 11.38PQGLLQDD109 pKa = 3.65EE110 pKa = 5.04DD111 pKa = 4.13EE112 pKa = 5.12LVDD115 pKa = 4.21EE116 pKa = 5.78KK117 pKa = 11.13IRR119 pKa = 11.84DD120 pKa = 3.66TNFAYY125 pKa = 9.61IDD127 pKa = 3.92LAVCDD132 pKa = 4.51EE133 pKa = 5.08GYY135 pKa = 8.42STANDD140 pKa = 3.33QGSPTVSHH148 pKa = 6.81RR149 pKa = 11.84SVEE152 pKa = 4.35EE153 pKa = 3.66TCMDD157 pKa = 3.88CTCIRR162 pKa = 11.84EE163 pKa = 4.21ILNQSLDD170 pKa = 4.15DD171 pKa = 4.44FPLSTSEE178 pKa = 4.1LSPAGFMDD186 pKa = 5.01LCDD189 pKa = 3.94EE190 pKa = 4.79SPDD193 pKa = 3.79FGNPYY198 pKa = 10.52DD199 pKa = 5.12DD200 pKa = 3.96EE201 pKa = 5.73LSFIDD206 pKa = 5.37LTDD209 pKa = 3.46EE210 pKa = 4.29TDD212 pKa = 3.62DD213 pKa = 5.02DD214 pKa = 4.25EE215 pKa = 6.82LMVIDD220 pKa = 4.57LTEE223 pKa = 4.78DD224 pKa = 2.89SWSNTSANEE233 pKa = 3.84EE234 pKa = 4.21SRR236 pKa = 11.84GEE238 pKa = 3.98NPIQVISDD246 pKa = 3.34FVIVGEE252 pKa = 4.41SLCPVYY258 pKa = 10.26KK259 pKa = 10.41YY260 pKa = 10.47HH261 pKa = 7.16GVDD264 pKa = 5.64FIDD267 pKa = 3.81LTLLL271 pKa = 3.75

Molecular weight: 30.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F7ZIL0|A0A0F7ZIL0_9HYPO DDE_3 domain-containing protein OS=Hirsutella minnesotensis 3608 OX=1043627 GN=HIM_11931 PE=4 SV=1
MM1 pKa = 7.32YY2 pKa = 9.25PGLCWQTLQTRR13 pKa = 11.84DD14 pKa = 3.84KK15 pKa = 11.05KK16 pKa = 10.96RR17 pKa = 11.84LDD19 pKa = 3.58LGRR22 pKa = 11.84VDD24 pKa = 3.63PRR26 pKa = 11.84AAIGPSSPIVRR37 pKa = 11.84RR38 pKa = 11.84GGTTRR43 pKa = 11.84TTTLPRR49 pKa = 11.84AAIGPSSPIVRR60 pKa = 11.84RR61 pKa = 11.84GGTTRR66 pKa = 11.84TTTLPRR72 pKa = 11.84AAIGPSSPIVRR83 pKa = 11.84RR84 pKa = 11.84GGTTRR89 pKa = 11.84TTTLPRR95 pKa = 11.84AAIGPSSPIVRR106 pKa = 11.84RR107 pKa = 11.84GGTTRR112 pKa = 11.84TTTLPRR118 pKa = 11.84AAIGPSSPIVRR129 pKa = 11.84RR130 pKa = 11.84GGTTRR135 pKa = 11.84TTTLPRR141 pKa = 11.84AAIGPSSPIVRR152 pKa = 11.84RR153 pKa = 11.84GGTTRR158 pKa = 11.84TTTLPRR164 pKa = 11.84AAIGPSSPIVRR175 pKa = 11.84RR176 pKa = 11.84GGTTRR181 pKa = 11.84TTTLPRR187 pKa = 11.84AAIGPSSPIVRR198 pKa = 11.84RR199 pKa = 11.84GGTTRR204 pKa = 11.84TTTLPRR210 pKa = 11.84AAIGPSSPIVRR221 pKa = 11.84RR222 pKa = 11.84GGTTRR227 pKa = 11.84TTTLPRR233 pKa = 11.84AAIGPSSPIVRR244 pKa = 11.84RR245 pKa = 11.84GGTTRR250 pKa = 11.84TTTLPRR256 pKa = 11.84AAIGPSSPIVRR267 pKa = 11.84RR268 pKa = 11.84GGTTRR273 pKa = 11.84TTTLPRR279 pKa = 11.84AAIGPSSPIVRR290 pKa = 11.84RR291 pKa = 11.84GGTTRR296 pKa = 11.84TTTLPRR302 pKa = 11.84AAIGPSSPIVRR313 pKa = 11.84RR314 pKa = 11.84GGTTRR319 pKa = 11.84TTTLPRR325 pKa = 11.84AAIGPSSPIVRR336 pKa = 11.84RR337 pKa = 11.84GGTTRR342 pKa = 11.84TTTLPRR348 pKa = 11.84AAIGPSSPIVRR359 pKa = 11.84RR360 pKa = 11.84GGTTRR365 pKa = 11.84TTTLPRR371 pKa = 11.84AAIGPSSPIVRR382 pKa = 11.84RR383 pKa = 11.84GGTTRR388 pKa = 11.84TTTLPRR394 pKa = 11.84AAIGPSSPIVRR405 pKa = 11.84RR406 pKa = 11.84GGTTRR411 pKa = 11.84TTTLPRR417 pKa = 11.84AAIGPSSPIVRR428 pKa = 11.84RR429 pKa = 11.84GGTTRR434 pKa = 11.84TTTLPRR440 pKa = 11.84AAIGPSSPIVRR451 pKa = 11.84RR452 pKa = 11.84GGTTRR457 pKa = 11.84TTTLPRR463 pKa = 11.84AAIGPSSPIVRR474 pKa = 11.84RR475 pKa = 11.84GGTTRR480 pKa = 11.84TTTLPRR486 pKa = 11.84AAIGPSSPIVRR497 pKa = 11.84RR498 pKa = 11.84GGTTRR503 pKa = 11.84TTTLPRR509 pKa = 11.84AAIGPSSPIVRR520 pKa = 11.84RR521 pKa = 11.84GGTTRR526 pKa = 11.84TTTLPRR532 pKa = 11.84AAIGPSSPIVRR543 pKa = 11.84RR544 pKa = 11.84GGTTRR549 pKa = 11.84TTTLPRR555 pKa = 11.84AAIGPSSPIVRR566 pKa = 11.84RR567 pKa = 11.84GGTTRR572 pKa = 11.84TTTLPRR578 pKa = 11.84AAIGPSSPIVRR589 pKa = 11.84RR590 pKa = 11.84GGTTRR595 pKa = 11.84TTTLPRR601 pKa = 11.84AAIGPSSPIVRR612 pKa = 11.84RR613 pKa = 11.84GGTTRR618 pKa = 11.84TTTLPRR624 pKa = 11.84AAIGPSSPIVRR635 pKa = 11.84RR636 pKa = 11.84GGTTRR641 pKa = 11.84TTTLPRR647 pKa = 11.84AAIGPSSPIVRR658 pKa = 11.84RR659 pKa = 11.84GGTTRR664 pKa = 11.84TTTLPRR670 pKa = 11.84AAIGPSSPIVRR681 pKa = 11.84RR682 pKa = 11.84GGTTRR687 pKa = 11.84TTTLPRR693 pKa = 11.84AAIGPSSPIVRR704 pKa = 11.84RR705 pKa = 11.84GGTTRR710 pKa = 11.84TTTLPRR716 pKa = 11.84AAIGPSSPIVRR727 pKa = 11.84RR728 pKa = 11.84GGTTRR733 pKa = 11.84TTTLPRR739 pKa = 11.84AAIGPSSPIVRR750 pKa = 11.84RR751 pKa = 11.84GGTTRR756 pKa = 11.84TTTLPRR762 pKa = 11.84AAIGPSSPIVRR773 pKa = 11.84RR774 pKa = 11.84GGTTRR779 pKa = 11.84TTTLPRR785 pKa = 11.84AAIGPSSPIVRR796 pKa = 11.84RR797 pKa = 11.84GGTTRR802 pKa = 11.84TTTLPRR808 pKa = 11.84AAIGPSSPIVRR819 pKa = 11.84RR820 pKa = 11.84GGTTRR825 pKa = 11.84TTTLPRR831 pKa = 11.84AAIGPSSPIVRR842 pKa = 11.84RR843 pKa = 11.84GGTTRR848 pKa = 11.84TTTLPRR854 pKa = 11.84AAIGPSSPIVRR865 pKa = 11.84RR866 pKa = 11.84GGTTRR871 pKa = 11.84TTTLPRR877 pKa = 11.84AATVAPWKK885 pKa = 10.2WPINPDD891 pKa = 3.11KK892 pKa = 11.36GFF894 pKa = 3.53

Molecular weight: 91.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9072

0

9072

4418322

50

8878

487.0

54.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.181 ± 0.028

1.4 ± 0.009

5.923 ± 0.019

6.005 ± 0.03

3.6 ± 0.014

6.812 ± 0.028

2.52 ± 0.011

4.466 ± 0.016

4.641 ± 0.02

9.001 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.155 ± 0.011

3.333 ± 0.014

5.993 ± 0.027

4.234 ± 0.019

7.106 ± 0.025

7.869 ± 0.033

5.56 ± 0.015

6.124 ± 0.02

1.54 ± 0.009

2.538 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski