Itacaiunas virus
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3R1F4|A0A0D3R1F4_9RHAB Phosphoprotein OS=Itacaiunas virus OX=490111 PE=4 SV=1
MM1 pKa = 8.17 DD2 pKa = 5.51 PPSYY6 pKa = 9.12 WVNGARR12 pKa = 11.84 PVLLRR17 pKa = 11.84 EE18 pKa = 4.25 SYY20 pKa = 10.4 PVGEE24 pKa = 3.99 QFIFRR29 pKa = 11.84 GFTSLLGSLNKK40 pKa = 10.42 ANIVNMLEE48 pKa = 4.05 HH49 pKa = 6.14 LQKK52 pKa = 10.52 FASSPIYY59 pKa = 10.56 YY60 pKa = 10.01 KK61 pKa = 10.27 IEE63 pKa = 4.2 DD64 pKa = 4.08 SILEE68 pKa = 3.98 EE69 pKa = 3.93 GAFFVTILDD78 pKa = 3.81 PQNPQHH84 pKa = 7.11 SSVV87 pKa = 3.31
Molecular weight: 9.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.054
IPC2_protein 5.219
IPC_protein 5.016
Toseland 5.003
ProMoST 5.169
Dawson 5.016
Bjellqvist 5.143
Wikipedia 4.889
Rodwell 4.952
Grimsley 4.94
Solomon 5.016
Lehninger 4.978
Nozaki 5.156
DTASelect 5.258
Thurlkill 5.016
EMBOSS 4.94
Sillero 5.232
Patrickios 3.834
IPC_peptide 5.029
IPC2_peptide 5.219
IPC2.peptide.svr19 5.275
Protein with the highest isoelectric point:
>tr|A0A0D3R127|A0A0D3R127_9RHAB Matrix OS=Itacaiunas virus OX=490111 PE=4 SV=1
MM1 pKa = 7.32 EE2 pKa = 5.06 NKK4 pKa = 10.41 AFGLDD9 pKa = 3.4 DD10 pKa = 4.59 LGAFFKK16 pKa = 11.26 NFITGISNQFNWFFKK31 pKa = 10.14 TVFSLVIIFLLIKK44 pKa = 10.26 ILVKK48 pKa = 10.56 FMSGLSNCIAAGFKK62 pKa = 9.59 IRR64 pKa = 11.84 RR65 pKa = 11.84 QVRR68 pKa = 11.84 HH69 pKa = 4.85 WRR71 pKa = 11.84 NGNEE75 pKa = 4.32 GSRR78 pKa = 11.84 EE79 pKa = 3.97 NPP81 pKa = 3.32
Molecular weight: 9.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.161
IPC2_protein 9.37
IPC_protein 9.458
Toseland 10.687
ProMoST 10.101
Dawson 10.73
Bjellqvist 10.292
Wikipedia 10.804
Rodwell 11.257
Grimsley 10.73
Solomon 10.789
Lehninger 10.789
Nozaki 10.657
DTASelect 10.277
Thurlkill 10.643
EMBOSS 11.052
Sillero 10.643
Patrickios 11.052
IPC_peptide 10.804
IPC2_peptide 8.829
IPC2.peptide.svr19 8.49
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
3951
81
2121
564.4
64.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.721 ± 0.576
1.696 ± 0.305
5.821 ± 0.463
6.707 ± 0.387
4.733 ± 0.535
6.454 ± 0.395
1.974 ± 0.103
6.074 ± 0.637
6.935 ± 0.268
10.301 ± 0.404
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.177 ± 0.391
4.91 ± 0.402
4.885 ± 0.372
2.632 ± 0.197
5.872 ± 0.393
9.086 ± 0.536
4.758 ± 0.609
6.201 ± 0.305
2.075 ± 0.168
2.987 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here