Bacillus obstructivus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4972 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L8ZHY5|A0A1L8ZHY5_9BACI Uncharacterized protein OS=Bacillus obstructivus OX=1914540 GN=BLX88_18045 PE=4 SV=1
MM1 pKa = 7.73AYY3 pKa = 10.4VITFPCQGEE12 pKa = 4.18KK13 pKa = 10.37AAEE16 pKa = 4.14CVEE19 pKa = 4.32VCPVDD24 pKa = 5.31CIAEE28 pKa = 4.41GKK30 pKa = 9.74DD31 pKa = 3.24QFYY34 pKa = 10.33IDD36 pKa = 4.43PDD38 pKa = 3.32ICIDD42 pKa = 3.95CGACQAVCPVDD53 pKa = 5.17AIFHH57 pKa = 5.75EE58 pKa = 4.69EE59 pKa = 3.94EE60 pKa = 4.53LLDD63 pKa = 3.71EE64 pKa = 4.58DD65 pKa = 3.8MEE67 pKa = 4.51YY68 pKa = 10.97FEE70 pKa = 5.06KK71 pKa = 10.76ARR73 pKa = 11.84KK74 pKa = 9.08FFTSS78 pKa = 3.04

Molecular weight: 8.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L8ZQ74|A0A1L8ZQ74_9BACI Chromosome partitioning protein ParB OS=Bacillus obstructivus OX=1914540 GN=BLX88_05770 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.28QPNARR10 pKa = 11.84KK11 pKa = 9.43RR12 pKa = 11.84SKK14 pKa = 10.14VHH16 pKa = 5.8GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.89VLANRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.69GRR39 pKa = 11.84KK40 pKa = 8.69VLSAA44 pKa = 4.05

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4972

0

4972

1385854

26

1659

278.7

31.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.282 ± 0.038

0.744 ± 0.011

4.929 ± 0.025

7.18 ± 0.037

4.724 ± 0.03

6.689 ± 0.038

2.15 ± 0.018

8.842 ± 0.038

7.432 ± 0.034

9.827 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.752 ± 0.017

4.808 ± 0.029

3.522 ± 0.018

3.761 ± 0.025

3.806 ± 0.029

6.113 ± 0.024

5.235 ± 0.026

6.53 ± 0.03

1.051 ± 0.014

3.622 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski