Broome virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Orthoreovirus; unclassified Orthoreovirus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D6MM30|D6MM30_9REOV Small non-structural protein p16 OS=Broome virus OX=667093 PE=4 SV=1
MM1 pKa = 7.7EE2 pKa = 5.05FPSFEE7 pKa = 3.78WLSFNEE13 pKa = 4.11LKK15 pKa = 10.65KK16 pKa = 9.64ITVGEE21 pKa = 4.22VAPFLCDD28 pKa = 3.64HH29 pKa = 6.65EE30 pKa = 5.04CKK32 pKa = 10.53AWMPFWIIMRR42 pKa = 11.84QEE44 pKa = 3.75SHH46 pKa = 6.84FEE48 pKa = 3.99GMSTLRR54 pKa = 11.84MVHH57 pKa = 6.89LFHH60 pKa = 6.75EE61 pKa = 4.84WVWMCLHH68 pKa = 6.54EE69 pKa = 4.9NEE71 pKa = 4.72CLRR74 pKa = 11.84NRR76 pKa = 11.84YY77 pKa = 6.29TLWFVEE83 pKa = 4.39CLQTRR88 pKa = 11.84FGDD91 pKa = 4.57LNNADD96 pKa = 3.48YY97 pKa = 10.18WIGRR101 pKa = 11.84RR102 pKa = 11.84FAKK105 pKa = 10.4FVYY108 pKa = 9.83DD109 pKa = 4.04RR110 pKa = 11.84CLLLQDD116 pKa = 4.66DD117 pKa = 4.8EE118 pKa = 4.41MMEE121 pKa = 4.08IDD123 pKa = 3.66VV124 pKa = 4.37

Molecular weight: 15.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D6MM30|D6MM30_9REOV Small non-structural protein p16 OS=Broome virus OX=667093 PE=4 SV=1
MM1 pKa = 7.64GSGPSNFVNKK11 pKa = 9.73VDD13 pKa = 4.12GASAPIKK20 pKa = 9.73EE21 pKa = 4.23HH22 pKa = 7.16AIPSLTSDD30 pKa = 3.83LKK32 pKa = 11.1DD33 pKa = 3.42YY34 pKa = 10.98LYY36 pKa = 10.6TIVTAVILLVILWFLYY52 pKa = 10.06RR53 pKa = 11.84YY54 pKa = 10.22YY55 pKa = 10.78KK56 pKa = 10.4DD57 pKa = 2.86KK58 pKa = 10.87KK59 pKa = 10.18ARR61 pKa = 11.84KK62 pKa = 8.67KK63 pKa = 10.94KK64 pKa = 10.35EE65 pKa = 4.39DD66 pKa = 3.23ILLRR70 pKa = 11.84LYY72 pKa = 11.06GRR74 pKa = 11.84GLNLSRR80 pKa = 11.84LDD82 pKa = 3.58PSVICSLGGSAPNLQHH98 pKa = 7.12RR99 pKa = 11.84GLEE102 pKa = 4.02RR103 pKa = 11.84TEE105 pKa = 4.48DD106 pKa = 3.46KK107 pKa = 10.92LVNPFII113 pKa = 5.36

Molecular weight: 12.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

7327

113

1297

666.1

75.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.22 ± 0.441

1.72 ± 0.133

6.278 ± 0.285

4.518 ± 0.404

4.067 ± 0.299

5.705 ± 0.138

2.443 ± 0.271

5.623 ± 0.233

3.439 ± 0.458

9.895 ± 0.391

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.344 ± 0.264

4.831 ± 0.279

5.077 ± 0.425

3.48 ± 0.18

6.497 ± 0.246

7.07 ± 0.344

5.528 ± 0.329

7.602 ± 0.208

1.61 ± 0.151

4.054 ± 0.343
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski