Leshenault partiti-like virus
Average proteome isoelectric point is 8.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z2RT46|A0A1Z2RT46_9VIRU RdRp OS=Leshenault partiti-like virus OX=2010278 PE=4 SV=1
MM1 pKa = 6.75 QASRR5 pKa = 11.84 MLEE8 pKa = 3.8 SHH10 pKa = 6.39 RR11 pKa = 11.84 VWGEE15 pKa = 3.59 YY16 pKa = 7.87 RR17 pKa = 11.84 THH19 pKa = 7.16 RR20 pKa = 11.84 LLEE23 pKa = 4.22 AHH25 pKa = 7.37 DD26 pKa = 5.42 GRR28 pKa = 11.84 ATWLRR33 pKa = 11.84 AWTKK37 pKa = 10.23 SWAPRR42 pKa = 11.84 QGVVEE47 pKa = 4.18 STPEE51 pKa = 3.78 AEE53 pKa = 4.01 KK54 pKa = 10.7 HH55 pKa = 5.5 VGVMTYY61 pKa = 10.32 VKK63 pKa = 9.87 GWRR66 pKa = 11.84 RR67 pKa = 11.84 RR68 pKa = 11.84 LRR70 pKa = 11.84 RR71 pKa = 11.84 QLRR74 pKa = 11.84 SARR77 pKa = 11.84 TQFEE81 pKa = 4.24 RR82 pKa = 11.84 EE83 pKa = 4.19 VVHH86 pKa = 6.55 GSWAALEE93 pKa = 4.18 GLYY96 pKa = 10.49 SEE98 pKa = 5.4 ALEE101 pKa = 4.81 HH102 pKa = 6.64 SPTMDD107 pKa = 3.24 LMYY110 pKa = 11.0 GHH112 pKa = 6.75 VLSLSVDD119 pKa = 3.49 RR120 pKa = 11.84 DD121 pKa = 3.41 HH122 pKa = 7.5 GFTRR126 pKa = 11.84 SFPSWYY132 pKa = 9.64 VSPAGFEE139 pKa = 3.78 NVPIVKK145 pKa = 8.82 RR146 pKa = 11.84 TTAKK150 pKa = 10.9 DD151 pKa = 3.1 PFTRR155 pKa = 11.84 MVEE158 pKa = 4.04 TGPTGIVARR167 pKa = 11.84 TMMKK171 pKa = 10.29 VGGRR175 pKa = 11.84 PQKK178 pKa = 10.03 FTDD181 pKa = 3.43 AAATSLAYY189 pKa = 9.41 KK190 pKa = 9.21 WLHH193 pKa = 5.59 ATGLWSSVTDD203 pKa = 3.67 ADD205 pKa = 4.04 VVINVSASFDD215 pKa = 3.4 TSRR218 pKa = 11.84 VHH220 pKa = 5.78 MEE222 pKa = 4.03 KK223 pKa = 9.68 FTAPRR228 pKa = 11.84 PPTEE232 pKa = 4.29 GTLDD236 pKa = 3.55 MCWEE240 pKa = 4.21 VVARR244 pKa = 11.84 NFKK247 pKa = 9.76 EE248 pKa = 4.0 LGMTGLPWITVADD261 pKa = 4.65 VIAPHH266 pKa = 6.76 INGDD270 pKa = 3.7 ACPGLLTSRR279 pKa = 11.84 IYY281 pKa = 9.73 RR282 pKa = 11.84 TRR284 pKa = 11.84 AQASSCTTSDD294 pKa = 3.21 VVALFEE300 pKa = 4.37 ALRR303 pKa = 11.84 TQEE306 pKa = 4.73 LLCALPWAAGGRR318 pKa = 11.84 EE319 pKa = 3.79 KK320 pKa = 10.69 RR321 pKa = 11.84 QEE323 pKa = 4.12 RR324 pKa = 11.84 LVGDD328 pKa = 3.72 QVSSRR333 pKa = 11.84 LVQMPEE339 pKa = 3.76 DD340 pKa = 3.38 VLARR344 pKa = 11.84 LEE346 pKa = 4.27 SAVAGPLLQRR356 pKa = 11.84 LKK358 pKa = 9.8 WVKK361 pKa = 10.39 RR362 pKa = 11.84 DD363 pKa = 3.16 IALGMSFFRR372 pKa = 11.84 GRR374 pKa = 11.84 AQRR377 pKa = 11.84 FFHH380 pKa = 7.19 RR381 pKa = 11.84 FDD383 pKa = 3.17 TCTHH387 pKa = 5.6 VKK389 pKa = 10.49 SFDD392 pKa = 2.93 WSAFDD397 pKa = 3.61 SSVGEE402 pKa = 4.12 TLIVASFSLLRR413 pKa = 11.84 ACYY416 pKa = 9.9 PEE418 pKa = 4.72 GQHH421 pKa = 6.6 ADD423 pKa = 3.31 NVFKK427 pKa = 11.13 YY428 pKa = 9.68 ILSAFVHH435 pKa = 5.85 KK436 pKa = 10.29 HH437 pKa = 5.92 LIVPQGDD444 pKa = 4.06 VIGMHH449 pKa = 6.74 RR450 pKa = 11.84 GIPSGSGLTSLVGSVANWLILRR472 pKa = 11.84 TTLTDD477 pKa = 3.28 ICPPLLAQRR486 pKa = 11.84 AEE488 pKa = 4.22 VAVLGDD494 pKa = 3.42 DD495 pKa = 3.58 TLIGFRR501 pKa = 11.84 GYY503 pKa = 11.14 DD504 pKa = 3.99 GIPTNAEE511 pKa = 3.44 FTARR515 pKa = 11.84 AFALWGVAAGPSPSMVYY532 pKa = 10.35 PEE534 pKa = 4.51 GPPRR538 pKa = 11.84 SVYY541 pKa = 10.5 LEE543 pKa = 3.97 RR544 pKa = 11.84 CQTFLSVTSWCGLPSWRR561 pKa = 11.84 NRR563 pKa = 11.84 EE564 pKa = 3.66 WWVMDD569 pKa = 3.04 MGYY572 pKa = 11.08 AKK574 pKa = 10.28 GQPDD578 pKa = 3.47 VANAQVAALEE588 pKa = 4.35 DD589 pKa = 3.65 AGFSGFAPQLRR600 pKa = 11.84 GTRR603 pKa = 11.84 RR604 pKa = 11.84 RR605 pKa = 11.84 FQKK608 pKa = 9.66 WLQKK612 pKa = 10.53 QPGAHH617 pKa = 6.2 MLHH620 pKa = 6.83 PEE622 pKa = 4.12 YY623 pKa = 11.08 VEE625 pKa = 4.02 STEE628 pKa = 4.54 TITQGWMARR637 pKa = 11.84 AHH639 pKa = 6.41 SSTGTAKK646 pKa = 10.35 EE647 pKa = 3.69 LAAFDD652 pKa = 4.07 WDD654 pKa = 4.49 SKK656 pKa = 10.39 GQHH659 pKa = 6.38 PGNWPTRR666 pKa = 11.84 WKK668 pKa = 10.34 VAAEE672 pKa = 3.99 AAPPPHH678 pKa = 6.76 KK679 pKa = 11.05 AEE681 pKa = 4.16 VLYY684 pKa = 10.07 GAPGTRR690 pKa = 11.84 TVFEE694 pKa = 5.02 LNWARR699 pKa = 11.84 TRR701 pKa = 11.84 KK702 pKa = 10.08 GKK704 pKa = 10.55 VGLRR708 pKa = 11.84 LPVYY712 pKa = 8.96 EE713 pKa = 4.52 GSRR716 pKa = 11.84 APVLAAEE723 pKa = 4.58 MFRR726 pKa = 11.84 YY727 pKa = 8.0 TRR729 pKa = 11.84 VGLARR734 pKa = 11.84 VSEE737 pKa = 4.02 EE738 pKa = 3.92 LYY740 pKa = 10.65 LRR742 pKa = 11.84 EE743 pKa = 4.18 SGIVPPDD750 pKa = 3.4 VRR752 pKa = 11.84 RR753 pKa = 11.84 GRR755 pKa = 11.84 AWKK758 pKa = 10.27 CGLAVAILALGAALGYY774 pKa = 8.38 TVYY777 pKa = 10.78 ARR779 pKa = 11.84 PPPKK783 pKa = 10.28 PPDD786 pKa = 3.55 PSVTVRR792 pKa = 11.84 TGGTSS797 pKa = 2.79
Molecular weight: 88.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.917
IPC2_protein 8.873
IPC_protein 8.873
Toseland 9.414
ProMoST 9.297
Dawson 9.736
Bjellqvist 9.531
Wikipedia 9.94
Rodwell 9.897
Grimsley 9.823
Solomon 9.765
Lehninger 9.721
Nozaki 9.619
DTASelect 9.472
Thurlkill 9.575
EMBOSS 9.867
Sillero 9.706
Patrickios 5.067
IPC_peptide 9.765
IPC2_peptide 8.375
IPC2.peptide.svr19 7.83
Protein with the highest isoelectric point:
>tr|A0A1Z2RT46|A0A1Z2RT46_9VIRU RdRp OS=Leshenault partiti-like virus OX=2010278 PE=4 SV=1
MM1 pKa = 6.75 QASRR5 pKa = 11.84 MLEE8 pKa = 3.8 SHH10 pKa = 6.39 RR11 pKa = 11.84 VWGEE15 pKa = 3.59 YY16 pKa = 7.87 RR17 pKa = 11.84 THH19 pKa = 7.16 RR20 pKa = 11.84 LLEE23 pKa = 4.22 AHH25 pKa = 7.37 DD26 pKa = 5.42 GRR28 pKa = 11.84 ATWLRR33 pKa = 11.84 AWTKK37 pKa = 10.23 SWAPRR42 pKa = 11.84 QGVVEE47 pKa = 4.18 STPEE51 pKa = 3.78 AEE53 pKa = 4.01 KK54 pKa = 10.7 HH55 pKa = 5.5 VGVMTYY61 pKa = 10.32 VKK63 pKa = 9.87 GWRR66 pKa = 11.84 RR67 pKa = 11.84 RR68 pKa = 11.84 LRR70 pKa = 11.84 RR71 pKa = 11.84 QLRR74 pKa = 11.84 SARR77 pKa = 11.84 TQFEE81 pKa = 4.24 RR82 pKa = 11.84 EE83 pKa = 4.19 VVHH86 pKa = 6.55 GSWAALEE93 pKa = 4.18 GLYY96 pKa = 10.49 SEE98 pKa = 5.4 ALEE101 pKa = 4.81 HH102 pKa = 6.64 SPTMDD107 pKa = 3.24 LMYY110 pKa = 11.0 GHH112 pKa = 6.75 VLSLSVDD119 pKa = 3.49 RR120 pKa = 11.84 DD121 pKa = 3.41 HH122 pKa = 7.5 GFTRR126 pKa = 11.84 SFPSWYY132 pKa = 9.64 VSPAGFEE139 pKa = 3.78 NVPIVKK145 pKa = 8.82 RR146 pKa = 11.84 TTAKK150 pKa = 10.9 DD151 pKa = 3.1 PFTRR155 pKa = 11.84 MVEE158 pKa = 4.04 TGPTGIVARR167 pKa = 11.84 TMMKK171 pKa = 10.29 VGGRR175 pKa = 11.84 PQKK178 pKa = 10.03 FTDD181 pKa = 3.43 AAATSLAYY189 pKa = 9.41 KK190 pKa = 9.21 WLHH193 pKa = 5.59 ATGLWSSVTDD203 pKa = 3.67 ADD205 pKa = 4.04 VVINVSASFDD215 pKa = 3.4 TSRR218 pKa = 11.84 VHH220 pKa = 5.78 MEE222 pKa = 4.03 KK223 pKa = 9.68 FTAPRR228 pKa = 11.84 PPTEE232 pKa = 4.29 GTLDD236 pKa = 3.55 MCWEE240 pKa = 4.21 VVARR244 pKa = 11.84 NFKK247 pKa = 9.76 EE248 pKa = 4.0 LGMTGLPWITVADD261 pKa = 4.65 VIAPHH266 pKa = 6.76 INGDD270 pKa = 3.7 ACPGLLTSRR279 pKa = 11.84 IYY281 pKa = 9.73 RR282 pKa = 11.84 TRR284 pKa = 11.84 AQASSCTTSDD294 pKa = 3.21 VVALFEE300 pKa = 4.37 ALRR303 pKa = 11.84 TQEE306 pKa = 4.73 LLCALPWAAGGRR318 pKa = 11.84 EE319 pKa = 3.79 KK320 pKa = 10.69 RR321 pKa = 11.84 QEE323 pKa = 4.12 RR324 pKa = 11.84 LVGDD328 pKa = 3.72 QVSSRR333 pKa = 11.84 LVQMPEE339 pKa = 3.76 DD340 pKa = 3.38 VLARR344 pKa = 11.84 LEE346 pKa = 4.27 SAVAGPLLQRR356 pKa = 11.84 LKK358 pKa = 9.8 WVKK361 pKa = 10.39 RR362 pKa = 11.84 DD363 pKa = 3.16 IALGMSFFRR372 pKa = 11.84 GRR374 pKa = 11.84 AQRR377 pKa = 11.84 FFHH380 pKa = 7.19 RR381 pKa = 11.84 FDD383 pKa = 3.17 TCTHH387 pKa = 5.6 VKK389 pKa = 10.49 SFDD392 pKa = 2.93 WSAFDD397 pKa = 3.61 SSVGEE402 pKa = 4.12 TLIVASFSLLRR413 pKa = 11.84 ACYY416 pKa = 9.9 PEE418 pKa = 4.72 GQHH421 pKa = 6.6 ADD423 pKa = 3.31 NVFKK427 pKa = 11.13 YY428 pKa = 9.68 ILSAFVHH435 pKa = 5.85 KK436 pKa = 10.29 HH437 pKa = 5.92 LIVPQGDD444 pKa = 4.06 VIGMHH449 pKa = 6.74 RR450 pKa = 11.84 GIPSGSGLTSLVGSVANWLILRR472 pKa = 11.84 TTLTDD477 pKa = 3.28 ICPPLLAQRR486 pKa = 11.84 AEE488 pKa = 4.22 VAVLGDD494 pKa = 3.42 DD495 pKa = 3.58 TLIGFRR501 pKa = 11.84 GYY503 pKa = 11.14 DD504 pKa = 3.99 GIPTNAEE511 pKa = 3.44 FTARR515 pKa = 11.84 AFALWGVAAGPSPSMVYY532 pKa = 10.35 PEE534 pKa = 4.51 GPPRR538 pKa = 11.84 SVYY541 pKa = 10.5 LEE543 pKa = 3.97 RR544 pKa = 11.84 CQTFLSVTSWCGLPSWRR561 pKa = 11.84 NRR563 pKa = 11.84 EE564 pKa = 3.66 WWVMDD569 pKa = 3.04 MGYY572 pKa = 11.08 AKK574 pKa = 10.28 GQPDD578 pKa = 3.47 VANAQVAALEE588 pKa = 4.35 DD589 pKa = 3.65 AGFSGFAPQLRR600 pKa = 11.84 GTRR603 pKa = 11.84 RR604 pKa = 11.84 RR605 pKa = 11.84 FQKK608 pKa = 9.66 WLQKK612 pKa = 10.53 QPGAHH617 pKa = 6.2 MLHH620 pKa = 6.83 PEE622 pKa = 4.12 YY623 pKa = 11.08 VEE625 pKa = 4.02 STEE628 pKa = 4.54 TITQGWMARR637 pKa = 11.84 AHH639 pKa = 6.41 SSTGTAKK646 pKa = 10.35 EE647 pKa = 3.69 LAAFDD652 pKa = 4.07 WDD654 pKa = 4.49 SKK656 pKa = 10.39 GQHH659 pKa = 6.38 PGNWPTRR666 pKa = 11.84 WKK668 pKa = 10.34 VAAEE672 pKa = 3.99 AAPPPHH678 pKa = 6.76 KK679 pKa = 11.05 AEE681 pKa = 4.16 VLYY684 pKa = 10.07 GAPGTRR690 pKa = 11.84 TVFEE694 pKa = 5.02 LNWARR699 pKa = 11.84 TRR701 pKa = 11.84 KK702 pKa = 10.08 GKK704 pKa = 10.55 VGLRR708 pKa = 11.84 LPVYY712 pKa = 8.96 EE713 pKa = 4.52 GSRR716 pKa = 11.84 APVLAAEE723 pKa = 4.58 MFRR726 pKa = 11.84 YY727 pKa = 8.0 TRR729 pKa = 11.84 VGLARR734 pKa = 11.84 VSEE737 pKa = 4.02 EE738 pKa = 3.92 LYY740 pKa = 10.65 LRR742 pKa = 11.84 EE743 pKa = 4.18 SGIVPPDD750 pKa = 3.4 VRR752 pKa = 11.84 RR753 pKa = 11.84 GRR755 pKa = 11.84 AWKK758 pKa = 10.27 CGLAVAILALGAALGYY774 pKa = 8.38 TVYY777 pKa = 10.78 ARR779 pKa = 11.84 PPPKK783 pKa = 10.28 PPDD786 pKa = 3.55 PSVTVRR792 pKa = 11.84 TGGTSS797 pKa = 2.79
Molecular weight: 88.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.917
IPC2_protein 8.873
IPC_protein 8.873
Toseland 9.414
ProMoST 9.297
Dawson 9.736
Bjellqvist 9.531
Wikipedia 9.94
Rodwell 9.897
Grimsley 9.823
Solomon 9.765
Lehninger 9.721
Nozaki 9.619
DTASelect 9.472
Thurlkill 9.575
EMBOSS 9.867
Sillero 9.706
Patrickios 5.067
IPC_peptide 9.765
IPC2_peptide 8.375
IPC2.peptide.svr19 7.83
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
797
797
797
797.0
88.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.414 ± 0.0
1.255 ± 0.0
4.015 ± 0.0
5.395 ± 0.0
3.764 ± 0.0
8.156 ± 0.0
2.76 ± 0.0
2.509 ± 0.0
3.388 ± 0.0
8.532 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.509 ± 0.0
1.38 ± 0.0
6.274 ± 0.0
3.011 ± 0.0
8.657 ± 0.0
6.775 ± 0.0
7.026 ± 0.0
8.281 ± 0.0
3.388 ± 0.0
2.509 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here