Helleborus net necrosis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B9UZ27|B9UZ27_9VIRU Capsid protein OS=Helleborus net necrosis virus OX=592206 PE=3 SV=1
MM1 pKa = 7.36QSLSCFIIAAAGSCILGYY19 pKa = 10.94LLLEE23 pKa = 4.22RR24 pKa = 11.84LFAVNDD30 pKa = 3.75PCTVHH35 pKa = 7.73ISGEE39 pKa = 4.14SVRR42 pKa = 11.84IIGCAFTPDD51 pKa = 4.31FIEE54 pKa = 4.23YY55 pKa = 10.7ARR57 pKa = 11.84TLKK60 pKa = 10.67PFDD63 pKa = 3.66HH64 pKa = 6.83GFVV67 pKa = 3.42

Molecular weight: 7.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B9UZ28|B9UZ28_9VIRU RNA silencing suppressor OS=Helleborus net necrosis virus OX=592206 PE=3 SV=1
MM1 pKa = 8.36RR2 pKa = 11.84IRR4 pKa = 11.84DD5 pKa = 3.68QKK7 pKa = 10.98RR8 pKa = 11.84ALCSVFSFYY17 pKa = 10.89CGNNRR22 pKa = 11.84NDD24 pKa = 3.79ADD26 pKa = 4.07VVGLIMSFVLSNAVGFGTSNYY47 pKa = 9.32AKK49 pKa = 10.16KK50 pKa = 10.38RR51 pKa = 11.84RR52 pKa = 11.84ARR54 pKa = 11.84QVNRR58 pKa = 11.84CWRR61 pKa = 11.84CYY63 pKa = 9.93RR64 pKa = 11.84VIGGLCLPKK73 pKa = 10.34NCNGVTCTPTSSNIASFILTGHH95 pKa = 6.67RR96 pKa = 11.84VLDD99 pKa = 3.59TRR101 pKa = 11.84SRR103 pKa = 11.84RR104 pKa = 11.84SRR106 pKa = 11.84CHH108 pKa = 5.46

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2782

67

1956

463.7

52.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.549 ± 0.745

2.876 ± 0.891

5.068 ± 0.62

6.183 ± 1.455

6.362 ± 0.762

6.039 ± 0.563

2.552 ± 0.461

4.996 ± 0.312

6.219 ± 0.99

10.029 ± 0.611

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.725 ± 0.315

4.601 ± 0.386

4.242 ± 0.597

2.66 ± 0.626

5.787 ± 1.482

7.836 ± 0.32

5.572 ± 0.313

5.572 ± 0.529

0.827 ± 0.187

3.307 ± 0.255
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski