Tico virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Tico phebovirus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482KEP2|A0A482KEP2_9VIRU Nonstructural protein OS=Tico virus OX=2559113 GN=NSs PE=4 SV=1
MM1 pKa = 7.33ASKK4 pKa = 10.9YY5 pKa = 10.73LFDD8 pKa = 4.55RR9 pKa = 11.84PVVSRR14 pKa = 11.84TSDD17 pKa = 3.01QLKK20 pKa = 10.32RR21 pKa = 11.84IFVHH25 pKa = 4.96YY26 pKa = 8.95TAFNKK31 pKa = 8.88VCKK34 pKa = 10.48YY35 pKa = 9.95PITRR39 pKa = 11.84FMSLEE44 pKa = 4.11VPTVYY49 pKa = 10.69YY50 pKa = 10.7AVVEE54 pKa = 4.3DD55 pKa = 4.86EE56 pKa = 5.05RR57 pKa = 11.84PSLPDD62 pKa = 4.15FYY64 pKa = 11.65RR65 pKa = 11.84MGQLPARR72 pKa = 11.84WGFPEE77 pKa = 4.87DD78 pKa = 3.41SQVTKK83 pKa = 10.72RR84 pKa = 11.84SPRR87 pKa = 11.84KK88 pKa = 9.75YY89 pKa = 9.85LGLSIGLSSLDD100 pKa = 3.22SKK102 pKa = 11.56DD103 pKa = 3.31YY104 pKa = 11.31VRR106 pKa = 11.84FDD108 pKa = 3.52EE109 pKa = 5.1PLLKK113 pKa = 10.55SAISWPLTYY122 pKa = 9.82PSHH125 pKa = 6.8VFLNLLVKK133 pKa = 10.27RR134 pKa = 11.84EE135 pKa = 4.11SMGPWMFKK143 pKa = 10.26SICATYY149 pKa = 9.25FCRR152 pKa = 11.84MTNCEE157 pKa = 4.58EE158 pKa = 3.74IDD160 pKa = 3.41IALVRR165 pKa = 11.84AHH167 pKa = 7.23KK168 pKa = 10.32IIINEE173 pKa = 4.02ATKK176 pKa = 10.04MGLDD180 pKa = 3.08ITQFSGEE187 pKa = 4.1NLLMEE192 pKa = 4.84ICHH195 pKa = 5.61IQCIKK200 pKa = 10.65LLEE203 pKa = 4.42AFSNEE208 pKa = 3.61PSEE211 pKa = 4.54GRR213 pKa = 11.84VHH215 pKa = 7.24SPIYY219 pKa = 9.83QLLRR223 pKa = 11.84MFSNDD228 pKa = 2.31IVVPSLRR235 pKa = 11.84SGSGDD240 pKa = 3.43GDD242 pKa = 3.78CPALVNEE249 pKa = 4.98CVTAFIDD256 pKa = 5.31GMNGDD261 pKa = 4.01INHH264 pKa = 7.25PSVVSQDD271 pKa = 3.58YY272 pKa = 9.8EE273 pKa = 3.9

Molecular weight: 31.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482KC17|A0A482KC17_9VIRU Glycoprotein OS=Tico virus OX=2559113 GN=GPC PE=4 SV=1
MM1 pKa = 8.01SDD3 pKa = 3.61YY4 pKa = 11.2QKK6 pKa = 11.07LAVQFASEE14 pKa = 4.68DD15 pKa = 3.78LDD17 pKa = 3.62HH18 pKa = 6.99TAIQQWVNEE27 pKa = 4.04FAYY30 pKa = 10.13EE31 pKa = 4.12GFDD34 pKa = 3.08ARR36 pKa = 11.84TVIKK40 pKa = 10.47LLQEE44 pKa = 4.13RR45 pKa = 11.84GGDD48 pKa = 3.28GWKK51 pKa = 10.44NDD53 pKa = 3.63AKK55 pKa = 11.22KK56 pKa = 10.24MIVLALTRR64 pKa = 11.84GNKK67 pKa = 7.19PAKK70 pKa = 8.89MIKK73 pKa = 10.05KK74 pKa = 8.69MSPEE78 pKa = 3.53GRR80 pKa = 11.84KK81 pKa = 7.14TVEE84 pKa = 3.96ALVSKK89 pKa = 10.95YY90 pKa = 10.72KK91 pKa = 9.7LTSGNPSRR99 pKa = 11.84NDD101 pKa = 3.14LTLSRR106 pKa = 11.84VAAALAGWTCQAAVIVQDD124 pKa = 3.78YY125 pKa = 11.0LPVTGKK131 pKa = 9.05TIDD134 pKa = 3.69AFSPNYY140 pKa = 8.5PRR142 pKa = 11.84TMMHH146 pKa = 6.96PSFAGLIDD154 pKa = 3.5PTLPRR159 pKa = 11.84DD160 pKa = 3.47ILEE163 pKa = 4.56TIINAHH169 pKa = 6.18SLFLYY174 pKa = 10.26NFSRR178 pKa = 11.84TINPSLRR185 pKa = 11.84GLSKK189 pKa = 11.4AEE191 pKa = 3.88VEE193 pKa = 4.35QSFGQPMRR201 pKa = 11.84AAINSTFISSAQRR214 pKa = 11.84RR215 pKa = 11.84LMLEE219 pKa = 3.76TLGIMDD225 pKa = 4.74QNMKK229 pKa = 10.31LAANVVKK236 pKa = 10.52AAAIYY241 pKa = 9.75EE242 pKa = 4.05AMYY245 pKa = 11.01

Molecular weight: 27.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

4000

245

2101

1000.0

112.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.35 ± 0.877

2.625 ± 0.786

5.55 ± 0.603

6.625 ± 0.449

4.5 ± 0.318

5.775 ± 0.252

2.675 ± 0.272

6.1 ± 0.377

5.95 ± 0.257

8.975 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.475 ± 0.389

4.0 ± 0.258

3.725 ± 0.508

3.475 ± 0.298

5.7 ± 0.151

10.125 ± 0.74

4.975 ± 0.239

6.55 ± 0.205

1.175 ± 0.08

2.675 ± 0.405
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski