Dactylococcopsis salina PCC 8305

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Dactylococcopsis; Dactylococcopsis salina

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3305 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9YS87|K9YS87_DACSA TYR_PHOSPHATASE_2 domain-containing protein OS=Dactylococcopsis salina PCC 8305 OX=13035 GN=Dacsa_0597 PE=4 SV=1
MM1 pKa = 7.56LKK3 pKa = 10.45DD4 pKa = 4.06IIQVIPKK11 pKa = 9.63EE12 pKa = 4.05NYY14 pKa = 8.89KK15 pKa = 10.79LYY17 pKa = 11.23LNFEE21 pKa = 4.91DD22 pKa = 3.82NCEE25 pKa = 3.96GVVDD29 pKa = 4.19ISQLVEE35 pKa = 3.95FSGIFAPLQDD45 pKa = 4.17LNYY48 pKa = 9.56FQTVKK53 pKa = 10.66VNPEE57 pKa = 3.16WGTIYY62 pKa = 10.25WSNGADD68 pKa = 3.59LDD70 pKa = 4.16SDD72 pKa = 3.82VLYY75 pKa = 11.01SVVISSSLDD84 pKa = 3.14QQ85 pKa = 4.3

Molecular weight: 9.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9YUY6|K9YUY6_DACSA 2-isopropylmalate synthase OS=Dactylococcopsis salina PCC 8305 OX=13035 GN=leuA PE=3 SV=1
MM1 pKa = 7.37AQQTLRR7 pKa = 11.84GTRR10 pKa = 11.84LKK12 pKa = 10.54QKK14 pKa = 9.76RR15 pKa = 11.84KK16 pKa = 8.58CGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84THH26 pKa = 6.3NGKK29 pKa = 9.28NVINARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.09KK38 pKa = 9.79GRR40 pKa = 11.84ARR42 pKa = 11.84LSVV45 pKa = 3.12

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3305

0

3305

1004375

29

11106

303.9

33.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.403 ± 0.052

0.895 ± 0.019

5.215 ± 0.079

7.432 ± 0.05

4.046 ± 0.036

6.973 ± 0.076

1.669 ± 0.032

6.609 ± 0.036

4.741 ± 0.062

10.711 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.787 ± 0.029

4.53 ± 0.067

4.612 ± 0.04

5.253 ± 0.049

5.019 ± 0.055

6.379 ± 0.035

5.95 ± 0.053

6.466 ± 0.043

1.359 ± 0.024

2.952 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski