Clostridium tyrobutyricum DIVETGP

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium tyrobutyricum

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3032 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6N1X2|W6N1X2_CLOTY Hydrolase (HAD superfamily) OS=Clostridium tyrobutyricum DIVETGP OX=1408889 GN=CTDIVETGP_0363 PE=4 SV=1
MM1 pKa = 7.46IFVEE5 pKa = 4.55AEE7 pKa = 3.58VDD9 pKa = 3.14KK10 pKa = 10.96DD11 pKa = 3.75VCIGCGMCPSVCPDD25 pKa = 3.25VFTMEE30 pKa = 5.21DD31 pKa = 3.36DD32 pKa = 4.14GKK34 pKa = 10.97AGTLVSEE41 pKa = 4.69IPNTSEE47 pKa = 3.99DD48 pKa = 3.49SARR51 pKa = 11.84EE52 pKa = 4.02ARR54 pKa = 11.84DD55 pKa = 3.51GCPVDD60 pKa = 5.2AISINEE66 pKa = 3.84

Molecular weight: 6.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6N6B5|W6N6B5_CLOTY D-3-phosphoglycerate dehydrogenase OS=Clostridium tyrobutyricum DIVETGP OX=1408889 GN=CTDIVETGP_1775 PE=3 SV=1
MM1 pKa = 7.45WMTYY5 pKa = 7.15QPKK8 pKa = 9.57KK9 pKa = 8.72KK10 pKa = 9.51QRR12 pKa = 11.84KK13 pKa = 8.04RR14 pKa = 11.84EE15 pKa = 3.69HH16 pKa = 6.25GFRR19 pKa = 11.84KK20 pKa = 9.97RR21 pKa = 11.84MRR23 pKa = 11.84TLSGRR28 pKa = 11.84NVIKK32 pKa = 10.4RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.89GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3032

0

3032

880759

37

2878

290.5

32.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.507 ± 0.048

1.301 ± 0.019

5.679 ± 0.035

6.37 ± 0.047

4.345 ± 0.036

6.473 ± 0.047

1.408 ± 0.017

10.246 ± 0.053

9.301 ± 0.046

8.588 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.683 ± 0.021

6.48 ± 0.049

2.902 ± 0.027

2.521 ± 0.022

3.55 ± 0.032

6.743 ± 0.042

4.741 ± 0.03

6.375 ± 0.038

0.675 ± 0.014

4.112 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski