Porphyromonas catoniae ATCC 51270
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1597 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Z4WRD9|Z4WRD9_9PORP OmpA family protein OS=Porphyromonas catoniae ATCC 51270 OX=887901 GN=HMPREF0636_1487 PE=4 SV=1
MM1 pKa = 8.26 AEE3 pKa = 4.31 EE4 pKa = 4.87 DD5 pKa = 4.39 LIGYY9 pKa = 9.9 DD10 pKa = 3.88 DD11 pKa = 4.29 AASVAFIRR19 pKa = 11.84 NYY21 pKa = 9.66 IPQEE25 pKa = 3.94 LKK27 pKa = 10.88 EE28 pKa = 4.52 LLSDD32 pKa = 3.78 DD33 pKa = 4.98 DD34 pKa = 3.41 IVYY37 pKa = 9.89 FVDD40 pKa = 6.12 LIYY43 pKa = 10.54 DD44 pKa = 3.81 YY45 pKa = 11.2 YY46 pKa = 10.95 EE47 pKa = 3.84 SRR49 pKa = 11.84 GYY51 pKa = 9.72 MDD53 pKa = 5.76 EE54 pKa = 5.37 DD55 pKa = 3.95 DD56 pKa = 4.25 QDD58 pKa = 4.01 SEE60 pKa = 4.23 EE61 pKa = 4.83 AIEE64 pKa = 4.01 VDD66 pKa = 3.11 EE67 pKa = 5.42 DD68 pKa = 3.82 EE69 pKa = 4.34 LVEE72 pKa = 4.15 YY73 pKa = 10.24 VVKK76 pKa = 10.57 NAKK79 pKa = 9.68 KK80 pKa = 10.17 DD81 pKa = 3.64 GVGKK85 pKa = 10.0 FEE87 pKa = 4.85 PDD89 pKa = 3.0 QIRR92 pKa = 11.84 FIVQGEE98 pKa = 4.25 LEE100 pKa = 4.39 YY101 pKa = 11.12 CDD103 pKa = 5.09 SINLFDD109 pKa = 4.01
Molecular weight: 12.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.605
IPC_protein 3.592
Toseland 3.376
ProMoST 3.719
Dawson 3.579
Bjellqvist 3.77
Wikipedia 3.516
Rodwell 3.427
Grimsley 3.287
Solomon 3.567
Lehninger 3.528
Nozaki 3.706
DTASelect 3.91
Thurlkill 3.439
EMBOSS 3.528
Sillero 3.719
Patrickios 0.947
IPC_peptide 3.567
IPC2_peptide 3.681
IPC2.peptide.svr19 3.686
Protein with the highest isoelectric point:
>tr|Z4WQR0|Z4WQR0_9PORP Endonuclease III OS=Porphyromonas catoniae ATCC 51270 OX=887901 GN=nth PE=3 SV=1
MM1 pKa = 7.68 AGLIEE6 pKa = 4.76 KK7 pKa = 10.09 DD8 pKa = 3.64 GKK10 pKa = 10.43 QSTMTVDD17 pKa = 2.86 SDD19 pKa = 4.24 VFFHH23 pKa = 7.42 EE24 pKa = 3.53 ILARR28 pKa = 11.84 VNSGKK33 pKa = 9.93 RR34 pKa = 11.84 VKK36 pKa = 10.12 IRR38 pKa = 11.84 AKK40 pKa = 9.91 GSSMLPLVRR49 pKa = 11.84 STKK52 pKa = 10.71 DD53 pKa = 3.3 VIFLSALTPDD63 pKa = 3.67 SLQEE67 pKa = 3.91 GRR69 pKa = 11.84 LLLVRR74 pKa = 11.84 IPRR77 pKa = 11.84 GYY79 pKa = 8.48 VLHH82 pKa = 6.43 RR83 pKa = 11.84 VEE85 pKa = 5.45 HH86 pKa = 5.88 IEE88 pKa = 3.73 GRR90 pKa = 11.84 RR91 pKa = 11.84 ITLRR95 pKa = 11.84 GDD97 pKa = 3.12 GNPYY101 pKa = 9.35 QRR103 pKa = 11.84 EE104 pKa = 4.15 RR105 pKa = 11.84 CTPDD109 pKa = 2.89 QVLAEE114 pKa = 4.12 ATAVQRR120 pKa = 11.84 GKK122 pKa = 10.16 HH123 pKa = 5.56 LIEE126 pKa = 4.79 LGSRR130 pKa = 11.84 LWWRR134 pKa = 11.84 YY135 pKa = 6.17 EE136 pKa = 3.87 HH137 pKa = 7.1 LWPKK141 pKa = 10.56 NDD143 pKa = 4.66 LIRR146 pKa = 11.84 RR147 pKa = 11.84 ICLALYY153 pKa = 10.3 RR154 pKa = 11.84 RR155 pKa = 11.84 TLLRR159 pKa = 11.84 WGLL162 pKa = 3.54
Molecular weight: 18.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.545
IPC_protein 10.306
Toseland 10.584
ProMoST 10.277
Dawson 10.687
Bjellqvist 10.394
Wikipedia 10.891
Rodwell 10.877
Grimsley 10.745
Solomon 10.804
Lehninger 10.76
Nozaki 10.57
DTASelect 10.394
Thurlkill 10.584
EMBOSS 10.994
Sillero 10.613
Patrickios 10.599
IPC_peptide 10.804
IPC2_peptide 9.355
IPC2.peptide.svr19 8.662
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1597
0
1597
580261
33
2403
363.3
40.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.946 ± 0.052
0.894 ± 0.02
5.143 ± 0.035
6.745 ± 0.057
4.003 ± 0.037
7.247 ± 0.047
2.255 ± 0.028
6.039 ± 0.05
5.146 ± 0.049
10.769 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.212 ± 0.023
3.394 ± 0.044
4.39 ± 0.033
3.629 ± 0.037
6.12 ± 0.052
6.782 ± 0.051
5.816 ± 0.043
6.224 ± 0.045
1.115 ± 0.021
4.131 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here