Cardinium endosymbiont cBtQ1 of Bemisia tabaci

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Amoebophilaceae; Candidatus Cardinium; unclassified Candidatus Cardinium; Cardinium endosymbiont of Bemisia tabaci

Average proteome isoelectric point is 7.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 694 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X5J9H5|X5J9H5_9BACT Uncharacterized protein OS=Cardinium endosymbiont cBtQ1 of Bemisia tabaci OX=1354314 GN=CHV_a0456 PE=4 SV=1
MM1 pKa = 7.23YY2 pKa = 7.73WTLEE6 pKa = 4.24LVSHH10 pKa = 6.99LEE12 pKa = 4.04DD13 pKa = 4.51APWPATKK20 pKa = 10.54DD21 pKa = 3.51EE22 pKa = 5.26LIDD25 pKa = 3.49YY26 pKa = 8.19AKK28 pKa = 10.6RR29 pKa = 11.84SGAPFEE35 pKa = 4.54VIRR38 pKa = 11.84NLEE41 pKa = 3.99EE42 pKa = 6.08LDD44 pKa = 4.34DD45 pKa = 5.25DD46 pKa = 5.1DD47 pKa = 5.54YY48 pKa = 11.45PYY50 pKa = 11.25TSIEE54 pKa = 4.66EE55 pKa = 3.94IWPDD59 pKa = 3.86YY60 pKa = 8.46PTGDD64 pKa = 3.45DD65 pKa = 4.39FIFNEE70 pKa = 4.66DD71 pKa = 3.24EE72 pKa = 4.26YY73 pKa = 11.92

Molecular weight: 8.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X5J9J8|X5J9J8_9BACT Inorganic pyrophosphatase OS=Cardinium endosymbiont cBtQ1 of Bemisia tabaci OX=1354314 GN=ppa PE=3 SV=1
MM1 pKa = 7.16HH2 pKa = 6.99QLIKK6 pKa = 10.74YY7 pKa = 9.62VEE9 pKa = 4.98AICNPQPARR18 pKa = 11.84KK19 pKa = 8.94FPNFKK24 pKa = 10.77AGDD27 pKa = 3.95TINVHH32 pKa = 6.35VKK34 pKa = 9.16IQEE37 pKa = 4.16GNKK40 pKa = 9.32VRR42 pKa = 11.84IQQFQGAVIQRR53 pKa = 11.84KK54 pKa = 8.64NPNTNGEE61 pKa = 4.32TFTVRR66 pKa = 11.84KK67 pKa = 8.95VSKK70 pKa = 9.47EE71 pKa = 3.81VGVEE75 pKa = 4.01RR76 pKa = 11.84IFPLLCPNIEE86 pKa = 4.25KK87 pKa = 10.36IEE89 pKa = 4.12VKK91 pKa = 10.25RR92 pKa = 11.84KK93 pKa = 8.73GRR95 pKa = 11.84VRR97 pKa = 11.84RR98 pKa = 11.84ARR100 pKa = 11.84IYY102 pKa = 9.92YY103 pKa = 10.29LRR105 pKa = 11.84GKK107 pKa = 9.1QGKK110 pKa = 8.45AARR113 pKa = 11.84IKK115 pKa = 9.99EE116 pKa = 4.05LRR118 pKa = 3.72

Molecular weight: 13.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

694

0

694

241061

38

1712

347.4

39.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.559 ± 0.102

1.325 ± 0.03

4.625 ± 0.052

5.163 ± 0.075

4.355 ± 0.068

5.991 ± 0.079

2.771 ± 0.041

8.283 ± 0.083

7.386 ± 0.095

10.835 ± 0.091

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.325 ± 0.035

4.327 ± 0.074

3.977 ± 0.049

4.265 ± 0.069

4.073 ± 0.051

6.015 ± 0.064

5.77 ± 0.057

6.117 ± 0.075

0.981 ± 0.034

3.855 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski