Vibrio phage AS51

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Colwellvirinae; Kaohsiungvirus; Vibrio virus AS51

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A067YIS9|A0A067YIS9_9CAUD Uncharacterized protein OS=Vibrio phage AS51 OX=1434127 PE=4 SV=1
MM1 pKa = 7.73DD2 pKa = 4.72VEE4 pKa = 5.27IIDD7 pKa = 3.95YY8 pKa = 11.06NSTDD12 pKa = 3.54VVDD15 pKa = 3.92LTGGKK20 pKa = 7.73TEE22 pKa = 4.58PEE24 pKa = 4.12DD25 pKa = 3.66PKK27 pKa = 10.92QEE29 pKa = 4.13QQEE32 pKa = 4.13QEE34 pKa = 4.32PEE36 pKa = 3.84KK37 pKa = 10.49STATEE42 pKa = 4.06SVKK45 pKa = 10.43PAEE48 pKa = 4.62EE49 pKa = 4.69SPASTPAEE57 pKa = 4.22DD58 pKa = 5.0ADD60 pKa = 3.97AEE62 pKa = 4.48SAATEE67 pKa = 3.99PEE69 pKa = 4.13KK70 pKa = 10.58TEE72 pKa = 3.94KK73 pKa = 10.79EE74 pKa = 4.1EE75 pKa = 4.53PEE77 pKa = 4.3DD78 pKa = 3.56SGEE81 pKa = 3.94EE82 pKa = 3.8PEE84 pKa = 4.56YY85 pKa = 10.72FFNGEE90 pKa = 4.03AVSVEE95 pKa = 4.07VPEE98 pKa = 4.38EE99 pKa = 3.65VSNALKK105 pKa = 10.27EE106 pKa = 4.06AEE108 pKa = 4.05IDD110 pKa = 3.74EE111 pKa = 4.62KK112 pKa = 11.59ALLSQLFKK120 pKa = 10.97KK121 pKa = 10.75DD122 pKa = 3.18GDD124 pKa = 3.77FSLDD128 pKa = 3.12EE129 pKa = 4.0EE130 pKa = 4.82TRR132 pKa = 11.84TKK134 pKa = 10.96LEE136 pKa = 4.21SKK138 pKa = 10.39FGKK141 pKa = 8.56TLVDD145 pKa = 4.87GYY147 pKa = 11.49LNMYY151 pKa = 10.45KK152 pKa = 10.57GMNEE156 pKa = 3.91QAIKK160 pKa = 10.39AAAADD165 pKa = 3.97KK166 pKa = 9.71EE167 pKa = 4.37QQEE170 pKa = 4.71VTSKK174 pKa = 10.1QQAEE178 pKa = 4.21EE179 pKa = 4.1FMTTVGGEE187 pKa = 4.03EE188 pKa = 4.36GLVAMEE194 pKa = 5.82DD195 pKa = 4.36FILKK199 pKa = 10.28NFDD202 pKa = 3.74DD203 pKa = 4.43KK204 pKa = 11.56QIAAYY209 pKa = 8.87NAVMEE214 pKa = 4.7SEE216 pKa = 4.2SHH218 pKa = 5.2EE219 pKa = 4.04QQLLIIQTVKK229 pKa = 11.12AQMEE233 pKa = 4.37LSDD236 pKa = 4.3KK237 pKa = 10.6LQNGDD242 pKa = 4.13KK243 pKa = 10.54NIKK246 pKa = 10.25LLGDD250 pKa = 4.04DD251 pKa = 4.16TSSTPTGTPLDD262 pKa = 3.86KK263 pKa = 11.11GYY265 pKa = 8.86LTSDD269 pKa = 3.73EE270 pKa = 4.81FQTIMDD276 pKa = 3.97SDD278 pKa = 4.2KK279 pKa = 9.94YY280 pKa = 8.83WTDD283 pKa = 3.6FEE285 pKa = 4.52YY286 pKa = 10.81QSKK289 pKa = 10.45VDD291 pKa = 3.65AARR294 pKa = 11.84SAGLKK299 pKa = 10.43RR300 pKa = 11.84NLL302 pKa = 3.73

Molecular weight: 33.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A067YII4|A0A067YII4_9CAUD N-acetyltransferase domain-containing protein OS=Vibrio phage AS51 OX=1434127 PE=4 SV=1
MM1 pKa = 7.21IRR3 pKa = 11.84SFGMTEE9 pKa = 3.62LRR11 pKa = 11.84KK12 pKa = 9.93AISSRR17 pKa = 11.84AVKK20 pKa = 10.61HH21 pKa = 6.3KK22 pKa = 10.69DD23 pKa = 3.49NPDD26 pKa = 3.27NLPNIKK32 pKa = 10.08KK33 pKa = 10.1EE34 pKa = 4.07LIRR37 pKa = 11.84RR38 pKa = 11.84QNGKK42 pKa = 9.97CPITGRR48 pKa = 11.84DD49 pKa = 3.81LRR51 pKa = 11.84SMTTTNVVVDD61 pKa = 4.4HH62 pKa = 6.36DD63 pKa = 4.44HH64 pKa = 6.44KK65 pKa = 11.31SGVIRR70 pKa = 11.84AALPRR75 pKa = 11.84AINGLEE81 pKa = 3.97GKK83 pKa = 8.2LTNLCIRR90 pKa = 11.84WGGCTSKK97 pKa = 10.9RR98 pKa = 11.84DD99 pKa = 4.02IINMLRR105 pKa = 11.84GMADD109 pKa = 3.46YY110 pKa = 11.31LEE112 pKa = 4.38LHH114 pKa = 5.9QVPQTEE120 pKa = 4.78WIHH123 pKa = 6.23PEE125 pKa = 3.72HH126 pKa = 6.5LTPAEE131 pKa = 3.84ARR133 pKa = 11.84AKK135 pKa = 10.41RR136 pKa = 11.84NAKK139 pKa = 9.14ARR141 pKa = 11.84KK142 pKa = 9.25RR143 pKa = 11.84YY144 pKa = 9.41ALKK147 pKa = 10.79KK148 pKa = 9.56KK149 pKa = 10.69DD150 pKa = 3.37KK151 pKa = 10.92

Molecular weight: 17.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34

0

34

12219

40

1229

359.4

40.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.159 ± 0.538

1.015 ± 0.138

6.416 ± 0.244

7.505 ± 0.413

3.945 ± 0.209

6.973 ± 0.273

2.046 ± 0.187

5.451 ± 0.204

7.251 ± 0.294

8.143 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.053 ± 0.213

4.853 ± 0.186

3.568 ± 0.175

4.092 ± 0.282

5.221 ± 0.372

5.852 ± 0.353

5.303 ± 0.348

6.032 ± 0.309

1.359 ± 0.154

3.765 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski