Amino acid dipepetide frequency for Streptomyces glaucescens

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.625AlaAla: 22.625 ± 0.186	1.072AlaCys: 1.072 ± 0.023	8.39AlaAsp: 8.39 ± 0.072	9.104AlaGlu: 9.104 ± 0.096	3.44AlaPhe: 3.44 ± 0.043	14.185AlaGly: 14.185 ± 0.114	3.061AlaHis: 3.061 ± 0.046	3.086AlaIle: 3.086 ± 0.042	2.559AlaLys: 2.559 ± 0.055	14.704AlaLeu: 14.704 ± 0.134	2.291AlaMet: 2.291 ± 0.039	1.777AlaAsn: 1.777 ± 0.034	7.945AlaPro: 7.945 ± 0.106	3.748AlaGln: 3.748 ± 0.047	11.517AlaArg: 11.517 ± 0.097	5.469AlaSer: 5.469 ± 0.059	6.943AlaThr: 6.943 ± 0.068	12.593AlaVal: 12.593 ± 0.101	1.926AlaTrp: 1.926 ± 0.035	2.852AlaTyr: 2.852 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.171CysAla: 1.171 ± 0.024	0.089CysCys: 0.089 ± 0.006	0.472CysAsp: 0.472 ± 0.014	0.406CysGlu: 0.406 ± 0.014	0.211CysPhe: 0.211 ± 0.01	0.938CysGly: 0.938 ± 0.024	0.209CysHis: 0.209 ± 0.01	0.136CysIle: 0.136 ± 0.008	0.102CysLys: 0.102 ± 0.007	0.74CysLeu: 0.74 ± 0.017	0.112CysMet: 0.112 ± 0.007	0.13CysAsn: 0.13 ± 0.008	0.486CysPro: 0.486 ± 0.016	0.154CysGln: 0.154 ± 0.008	0.611CysArg: 0.611 ± 0.019	0.419CysSer: 0.419 ± 0.015	0.498CysThr: 0.498 ± 0.016	0.657CysVal: 0.657 ± 0.02	0.131CysTrp: 0.131 ± 0.007	0.146CysTyr: 0.146 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.771AspAla: 7.771 ± 0.067	0.424AspCys: 0.424 ± 0.013	3.626AspAsp: 3.626 ± 0.052	3.926AspGlu: 3.926 ± 0.05	1.62AspPhe: 1.62 ± 0.031	6.718AspGly: 6.718 ± 0.058	1.415AspHis: 1.415 ± 0.024	1.779AspIle: 1.779 ± 0.032	1.117AspLys: 1.117 ± 0.029	6.181AspLeu: 6.181 ± 0.07	0.757AspMet: 0.757 ± 0.018	0.92AspAsn: 0.92 ± 0.023	4.837AspPro: 4.837 ± 0.053	1.399AspGln: 1.399 ± 0.028	5.163AspArg: 5.163 ± 0.054	2.28AspSer: 2.28 ± 0.036	3.346AspThr: 3.346 ± 0.044	4.736AspVal: 4.736 ± 0.052	1.068AspTrp: 1.068 ± 0.021	1.127AspTyr: 1.127 ± 0.024	0.0AspXaa: 0.0 ± 0.0
Glu	7.434GluAla: 7.434 ± 0.085	0.364GluCys: 0.364 ± 0.013	3.001GluAsp: 3.001 ± 0.04	3.64GluGlu: 3.64 ± 0.056	1.425GluPhe: 1.425 ± 0.025	4.434GluGly: 4.434 ± 0.047	1.487GluHis: 1.487 ± 0.029	2.158GluIle: 2.158 ± 0.04	1.42GluLys: 1.42 ± 0.029	6.733GluLeu: 6.733 ± 0.07	0.845GluMet: 0.845 ± 0.02	0.978GluAsn: 0.978 ± 0.025	3.651GluPro: 3.651 ± 0.056	2.149GluGln: 2.149 ± 0.033	5.895GluArg: 5.895 ± 0.064	2.374GluSer: 2.374 ± 0.035	2.967GluThr: 2.967 ± 0.045	4.464GluVal: 4.464 ± 0.052	0.741GluTrp: 0.741 ± 0.019	1.141GluTyr: 1.141 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	3.522PheAla: 3.522 ± 0.037	0.253PheCys: 0.253 ± 0.011	1.891PheAsp: 1.891 ± 0.034	1.414PheGlu: 1.414 ± 0.029	0.841PhePhe: 0.841 ± 0.021	2.926PheGly: 2.926 ± 0.041	0.61PheHis: 0.61 ± 0.018	0.603PheIle: 0.603 ± 0.02	0.463PheLys: 0.463 ± 0.015	2.547PheLeu: 2.547 ± 0.038	0.37PheMet: 0.37 ± 0.015	0.515PheAsn: 0.515 ± 0.016	1.376PhePro: 1.376 ± 0.025	0.659PheGln: 0.659 ± 0.018	1.935PheArg: 1.935 ± 0.036	1.356PheSer: 1.356 ± 0.028	1.975PheThr: 1.975 ± 0.031	2.155PheVal: 2.155 ± 0.032	0.396PheTrp: 0.396 ± 0.014	0.559PheTyr: 0.559 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	11.48GlyAla: 11.48 ± 0.099	0.859GlyCys: 0.859 ± 0.022	5.51GlyAsp: 5.51 ± 0.059	5.313GlyGlu: 5.313 ± 0.049	2.828GlyPhe: 2.828 ± 0.037	9.215GlyGly: 9.215 ± 0.1	2.419GlyHis: 2.419 ± 0.036	3.282GlyIle: 3.282 ± 0.042	2.253GlyLys: 2.253 ± 0.041	9.325GlyLeu: 9.325 ± 0.069	1.946GlyMet: 1.946 ± 0.034	1.718GlyAsn: 1.718 ± 0.036	5.787GlyPro: 5.787 ± 0.072	2.588GlyGln: 2.588 ± 0.041	8.609GlyArg: 8.609 ± 0.071	5.141GlySer: 5.141 ± 0.058	6.867GlyThr: 6.867 ± 0.076	7.398GlyVal: 7.398 ± 0.062	1.738GlyTrp: 1.738 ± 0.032	2.296GlyTyr: 2.296 ± 0.032	0.0GlyXaa: 0.0 ± 0.0
His	2.781HisAla: 2.781 ± 0.035	0.207HisCys: 0.207 ± 0.01	1.388HisAsp: 1.388 ± 0.028	1.208HisGlu: 1.208 ± 0.023	0.643HisPhe: 0.643 ± 0.017	2.535HisGly: 2.535 ± 0.036	0.707HisHis: 0.707 ± 0.02	0.62HisIle: 0.62 ± 0.016	0.336HisLys: 0.336 ± 0.013	2.431HisLeu: 2.431 ± 0.038	0.306HisMet: 0.306 ± 0.013	0.361HisAsn: 0.361 ± 0.014	1.944HisPro: 1.944 ± 0.035	0.629HisGln: 0.629 ± 0.017	2.257HisArg: 2.257 ± 0.038	0.951HisSer: 0.951 ± 0.022	1.427HisThr: 1.427 ± 0.024	1.703HisVal: 1.703 ± 0.029	0.383HisTrp: 0.383 ± 0.012	0.478HisTyr: 0.478 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	4.39IleAla: 4.39 ± 0.052	0.248IleCys: 0.248 ± 0.01	2.068IleAsp: 2.068 ± 0.036	1.844IleGlu: 1.844 ± 0.036	0.604IlePhe: 0.604 ± 0.018	3.264IleGly: 3.264 ± 0.049	0.561IleHis: 0.561 ± 0.019	0.753IleIle: 0.753 ± 0.02	0.64IleLys: 0.64 ± 0.021	2.156IleLeu: 2.156 ± 0.035	0.402IleMet: 0.402 ± 0.014	0.633IleAsn: 0.633 ± 0.02	1.637IlePro: 1.637 ± 0.029	0.663IleGln: 0.663 ± 0.019	2.169IleArg: 2.169 ± 0.036	1.46IleSer: 1.46 ± 0.031	2.126IleThr: 2.126 ± 0.033	2.563IleVal: 2.563 ± 0.038	0.345IleTrp: 0.345 ± 0.015	0.458IleTyr: 0.458 ± 0.013	0.0IleXaa: 0.0 ± 0.0
Lys	2.749LysAla: 2.749 ± 0.049	0.109LysCys: 0.109 ± 0.008	1.273LysAsp: 1.273 ± 0.033	1.119LysGlu: 1.119 ± 0.026	0.424LysPhe: 0.424 ± 0.015	1.732LysGly: 1.732 ± 0.033	0.39LysHis: 0.39 ± 0.015	0.773LysIle: 0.773 ± 0.022	0.763LysLys: 0.763 ± 0.031	1.881LysLeu: 1.881 ± 0.033	0.347LysMet: 0.347 ± 0.014	0.471LysAsn: 0.471 ± 0.017	1.236LysPro: 1.236 ± 0.028	0.663LysGln: 0.663 ± 0.023	1.372LysArg: 1.372 ± 0.027	0.988LysSer: 0.988 ± 0.027	1.177LysThr: 1.177 ± 0.031	1.773LysVal: 1.773 ± 0.036	0.244LysTrp: 0.244 ± 0.011	0.427LysTyr: 0.427 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	15.463LeuAla: 15.463 ± 0.126	0.831LeuCys: 0.831 ± 0.019	6.749LeuAsp: 6.749 ± 0.063	4.724LeuGlu: 4.724 ± 0.057	2.501LeuPhe: 2.501 ± 0.033	9.187LeuGly: 9.187 ± 0.076	2.305LeuHis: 2.305 ± 0.04	2.978LeuIle: 2.978 ± 0.043	1.924LeuLys: 1.924 ± 0.04	11.403LeuLeu: 11.403 ± 0.114	1.541LeuMet: 1.541 ± 0.028	1.535LeuAsn: 1.535 ± 0.028	6.669LeuPro: 6.669 ± 0.072	2.114LeuGln: 2.114 ± 0.032	8.98LeuArg: 8.98 ± 0.093	4.898LeuSer: 4.898 ± 0.052	6.889LeuThr: 6.889 ± 0.064	8.568LeuVal: 8.568 ± 0.084	1.288LeuTrp: 1.288 ± 0.033	1.827LeuTyr: 1.827 ± 0.029	0.0LeuXaa: 0.0 ± 0.0
Met	2.097MetAla: 2.097 ± 0.029	0.139MetCys: 0.139 ± 0.007	0.826MetAsp: 0.826 ± 0.02	0.736MetGlu: 0.736 ± 0.019	0.414MetPhe: 0.414 ± 0.014	1.221MetGly: 1.221 ± 0.026	0.321MetHis: 0.321 ± 0.012	0.602MetIle: 0.602 ± 0.018	0.386MetLys: 0.386 ± 0.015	1.512MetLeu: 1.512 ± 0.025	0.276MetMet: 0.276 ± 0.012	0.397MetAsn: 0.397 ± 0.013	1.045MetPro: 1.045 ± 0.023	0.399MetGln: 0.399 ± 0.014	1.387MetArg: 1.387 ± 0.027	1.258MetSer: 1.258 ± 0.025	1.478MetThr: 1.478 ± 0.025	1.149MetVal: 1.149 ± 0.026	0.206MetTrp: 0.206 ± 0.009	0.32MetTyr: 0.32 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.055AsnAla: 2.055 ± 0.038	0.163AsnCys: 0.163 ± 0.008	0.895AsnAsp: 0.895 ± 0.021	0.791AsnGlu: 0.791 ± 0.02	0.438AsnPhe: 0.438 ± 0.015	1.811AsnGly: 1.811 ± 0.034	0.386AsnHis: 0.386 ± 0.013	0.572AsnIle: 0.572 ± 0.017	0.393AsnLys: 0.393 ± 0.017	1.525AsnLeu: 1.525 ± 0.031	0.26AsnMet: 0.26 ± 0.012	0.425AsnAsn: 0.425 ± 0.016	1.294AsnPro: 1.294 ± 0.027	0.456AsnGln: 0.456 ± 0.018	1.222AsnArg: 1.222 ± 0.024	0.815AsnSer: 0.815 ± 0.024	1.04AsnThr: 1.04 ± 0.025	1.327AsnVal: 1.327 ± 0.029	0.279AsnTrp: 0.279 ± 0.013	0.386AsnTyr: 0.386 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	10.076ProAla: 10.076 ± 0.111	0.359ProCys: 0.359 ± 0.011	4.658ProAsp: 4.658 ± 0.057	4.579ProGlu: 4.579 ± 0.055	1.509ProPhe: 1.509 ± 0.026	7.678ProGly: 7.678 ± 0.086	1.509ProHis: 1.509 ± 0.032	1.096ProIle: 1.096 ± 0.024	1.047ProLys: 1.047 ± 0.025	5.505ProLeu: 5.505 ± 0.056	0.966ProMet: 0.966 ± 0.025	0.834ProAsn: 0.834 ± 0.022	4.126ProPro: 4.126 ± 0.073	1.78ProGln: 1.78 ± 0.04	4.624ProArg: 4.624 ± 0.055	3.083ProSer: 3.083 ± 0.044	2.963ProThr: 2.963 ± 0.043	5.719ProVal: 5.719 ± 0.056	0.941ProTrp: 0.941 ± 0.023	1.481ProTyr: 1.481 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	3.688GlnAla: 3.688 ± 0.048	0.17GlnCys: 0.17 ± 0.008	1.39GlnAsp: 1.39 ± 0.025	1.449GlnGlu: 1.449 ± 0.026	0.608GlnPhe: 0.608 ± 0.016	2.262GlnGly: 2.262 ± 0.037	0.63GlnHis: 0.63 ± 0.018	0.968GlnIle: 0.968 ± 0.025	0.557GlnLys: 0.557 ± 0.018	2.756GlnLeu: 2.756 ± 0.041	0.441GlnMet: 0.441 ± 0.016	0.458GlnAsn: 0.458 ± 0.018	1.762GlnPro: 1.762 ± 0.045	1.156GlnGln: 1.156 ± 0.034	2.365GlnArg: 2.365 ± 0.039	1.164GlnSer: 1.164 ± 0.022	1.272GlnThr: 1.272 ± 0.024	2.285GlnVal: 2.285 ± 0.036	0.475GlnTrp: 0.475 ± 0.015	0.602GlnTyr: 0.602 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	11.024ArgAla: 11.024 ± 0.109	0.609ArgCys: 0.609 ± 0.018	4.591ArgAsp: 4.591 ± 0.048	4.998ArgGlu: 4.998 ± 0.055	2.393ArgPhe: 2.393 ± 0.038	6.24ArgGly: 6.24 ± 0.065	2.321ArgHis: 2.321 ± 0.038	3.213ArgIle: 3.213 ± 0.039	1.624ArgLys: 1.624 ± 0.031	9.416ArgLeu: 9.416 ± 0.082	1.737ArgMet: 1.737 ± 0.028	1.346ArgAsn: 1.346 ± 0.027	5.746ArgPro: 5.746 ± 0.068	2.301ArgGln: 2.301 ± 0.035	8.608ArgArg: 8.608 ± 0.084	4.0ArgSer: 4.0 ± 0.049	5.919ArgThr: 5.919 ± 0.062	6.189ArgVal: 6.189 ± 0.055	1.445ArgTrp: 1.445 ± 0.029	1.821ArgTyr: 1.821 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	6.369SerAla: 6.369 ± 0.063	0.39SerCys: 0.39 ± 0.014	2.449SerAsp: 2.449 ± 0.041	2.143SerGlu: 2.143 ± 0.037	1.413SerPhe: 1.413 ± 0.025	5.735SerGly: 5.735 ± 0.071	0.978SerHis: 0.978 ± 0.021	1.216SerIle: 1.216 ± 0.025	0.888SerLys: 0.888 ± 0.028	4.491SerLeu: 4.491 ± 0.052	0.933SerMet: 0.933 ± 0.019	0.762SerAsn: 0.762 ± 0.021	3.113SerPro: 3.113 ± 0.045	1.124SerGln: 1.124 ± 0.023	3.659SerArg: 3.659 ± 0.039	2.46SerSer: 2.46 ± 0.044	2.793SerThr: 2.793 ± 0.042	4.004SerVal: 4.004 ± 0.042	0.831SerTrp: 0.831 ± 0.021	1.165SerTyr: 1.165 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	9.462ThrAla: 9.462 ± 0.076	0.432ThrCys: 0.432 ± 0.015	3.652ThrAsp: 3.652 ± 0.045	3.293ThrGlu: 3.293 ± 0.045	1.566ThrPhe: 1.566 ± 0.027	7.097ThrGly: 7.097 ± 0.067	1.221ThrHis: 1.221 ± 0.023	1.512ThrIle: 1.512 ± 0.032	1.063ThrLys: 1.063 ± 0.031	5.64ThrLeu: 5.64 ± 0.054	0.848ThrMet: 0.848 ± 0.022	0.889ThrAsn: 0.889 ± 0.023	4.359ThrPro: 4.359 ± 0.053	1.238ThrGln: 1.238 ± 0.03	4.197ThrArg: 4.197 ± 0.048	2.955ThrSer: 2.955 ± 0.043	3.84ThrThr: 3.84 ± 0.067	6.201ThrVal: 6.201 ± 0.061	0.914ThrTrp: 0.914 ± 0.024	1.37ThrTyr: 1.37 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	10.534ValAla: 10.534 ± 0.082	0.767ValCys: 0.767 ± 0.02	4.939ValAsp: 4.939 ± 0.048	4.575ValGlu: 4.575 ± 0.048	2.381ValPhe: 2.381 ± 0.036	6.017ValGly: 6.017 ± 0.057	1.997ValHis: 1.997 ± 0.031	2.658ValIle: 2.658 ± 0.031	1.638ValLys: 1.638 ± 0.033	9.61ValLeu: 9.61 ± 0.094	1.31ValMet: 1.31 ± 0.026	1.572ValAsn: 1.572 ± 0.029	5.664ValPro: 5.664 ± 0.057	2.018ValGln: 2.018 ± 0.03	7.68ValArg: 7.68 ± 0.078	4.042ValSer: 4.042 ± 0.05	5.929ValThr: 5.929 ± 0.058	7.793ValVal: 7.793 ± 0.087	1.187ValTrp: 1.187 ± 0.026	1.574ValTyr: 1.574 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.728TrpAla: 1.728 ± 0.031	0.159TrpCys: 0.159 ± 0.008	0.831TrpAsp: 0.831 ± 0.02	0.75TrpGlu: 0.75 ± 0.02	0.493TrpPhe: 0.493 ± 0.017	1.041TrpGly: 1.041 ± 0.023	0.376TrpHis: 0.376 ± 0.013	0.525TrpIle: 0.525 ± 0.016	0.357TrpLys: 0.357 ± 0.014	1.775TrpLeu: 1.775 ± 0.035	0.254TrpMet: 0.254 ± 0.011	0.415TrpAsn: 0.415 ± 0.015	0.799TrpPro: 0.799 ± 0.022	0.632TrpGln: 0.632 ± 0.019	1.398TrpArg: 1.398 ± 0.029	0.909TrpSer: 0.909 ± 0.024	1.1TrpThr: 1.1 ± 0.028	0.969TrpVal: 0.969 ± 0.026	0.343TrpTrp: 0.343 ± 0.014	0.373TrpTyr: 0.373 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.869TyrAla: 2.869 ± 0.035	0.175TyrCys: 0.175 ± 0.008	1.612TyrAsp: 1.612 ± 0.04	1.288TyrGlu: 1.288 ± 0.028	0.614TyrPhe: 0.614 ± 0.019	2.334TyrGly: 2.334 ± 0.038	0.391TyrHis: 0.391 ± 0.013	0.454TyrIle: 0.454 ± 0.015	0.382TyrLys: 0.382 ± 0.014	2.045TyrLeu: 2.045 ± 0.03	0.228TyrMet: 0.228 ± 0.011	0.396TyrAsn: 0.396 ± 0.017	1.057TyrPro: 1.057 ± 0.024	0.588TyrGln: 0.588 ± 0.017	1.892TyrArg: 1.892 ± 0.031	0.894TyrSer: 0.894 ± 0.024	1.198TyrThr: 1.198 ± 0.029	1.638TyrVal: 1.638 ± 0.028	0.346TyrTrp: 0.346 ± 0.014	0.46TyrTyr: 0.46 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6550 proteins (2194113 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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