Human genital-associated circular DNA virus-1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemygorvirus; Gemygorvirus sewopo1; Sewage derived gemygorvirus 1

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3DBM9|A0A0E3DBM9_9VIRU Capsid protein OS=Human genital-associated circular DNA virus-1 OX=1488574 PE=4 SV=1
MM1 pKa = 7.68AEE3 pKa = 4.03SPFRR7 pKa = 11.84VQSRR11 pKa = 11.84YY12 pKa = 9.12VLITYY17 pKa = 7.44AQCGEE22 pKa = 4.12LDD24 pKa = 3.3PWAVHH29 pKa = 7.07DD30 pKa = 5.8SITSYY35 pKa = 10.23PAEE38 pKa = 3.97CLIGRR43 pKa = 11.84EE44 pKa = 3.92NHH46 pKa = 6.51ADD48 pKa = 3.55GGIHH52 pKa = 6.42LHH54 pKa = 6.48AFVDD58 pKa = 4.58FGRR61 pKa = 11.84RR62 pKa = 11.84INLRR66 pKa = 11.84DD67 pKa = 3.48PRR69 pKa = 11.84RR70 pKa = 11.84FDD72 pKa = 3.43VDD74 pKa = 3.69DD75 pKa = 3.85RR76 pKa = 11.84HH77 pKa = 6.96PNIQPCGRR85 pKa = 11.84TPQKK89 pKa = 10.33MLDD92 pKa = 3.61YY93 pKa = 10.33AIKK96 pKa = 10.91DD97 pKa = 3.49GDD99 pKa = 4.03VVAGGLSTRR108 pKa = 11.84IGTEE112 pKa = 3.27IQGTDD117 pKa = 3.56NVWSRR122 pKa = 11.84IADD125 pKa = 3.58AASVEE130 pKa = 4.45EE131 pKa = 4.24FWEE134 pKa = 4.42LVRR137 pKa = 11.84EE138 pKa = 4.31LAPRR142 pKa = 11.84ALLCNHH148 pKa = 5.94QSLRR152 pKa = 11.84AYY154 pKa = 10.25AEE156 pKa = 3.52WHH158 pKa = 5.46YY159 pKa = 10.89RR160 pKa = 11.84PPAVAYY166 pKa = 6.77EE167 pKa = 4.33HH168 pKa = 7.2PPGLQLCTGKK178 pKa = 10.46FPEE181 pKa = 4.44LDD183 pKa = 2.66AWVRR187 pKa = 11.84DD188 pKa = 3.56NLLILWGEE196 pKa = 4.02TRR198 pKa = 11.84LGKK201 pKa = 7.89TVWARR206 pKa = 11.84SLGPHH211 pKa = 5.97IHH213 pKa = 7.3CCLQWNVDD221 pKa = 3.84DD222 pKa = 5.15LKK224 pKa = 11.47SGLEE228 pKa = 3.87DD229 pKa = 2.87AKK231 pKa = 11.26YY232 pKa = 10.73AVLDD236 pKa = 4.77DD237 pKa = 3.68IQGNFQFFPAYY248 pKa = 9.6KK249 pKa = 9.57GWLGGQSTFTVTDD262 pKa = 3.89KK263 pKa = 11.39YY264 pKa = 10.75RR265 pKa = 11.84GKK267 pKa = 7.59TTINWGRR274 pKa = 11.84PTIWLMNDD282 pKa = 3.12DD283 pKa = 4.37PEE285 pKa = 4.59EE286 pKa = 4.23VGHH289 pKa = 7.34VDD291 pKa = 4.77FNWLQGNCDD300 pKa = 3.97IIHH303 pKa = 6.02LTEE306 pKa = 4.03SLIVV310 pKa = 3.41

Molecular weight: 35.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3DBM9|A0A0E3DBM9_9VIRU Capsid protein OS=Human genital-associated circular DNA virus-1 OX=1488574 PE=4 SV=1
MM1 pKa = 7.4PRR3 pKa = 11.84RR4 pKa = 11.84YY5 pKa = 7.91TRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84ARR13 pKa = 11.84TSRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84STFRR22 pKa = 11.84KK23 pKa = 7.52TVTTRR28 pKa = 11.84GRR30 pKa = 11.84RR31 pKa = 11.84TRR33 pKa = 11.84KK34 pKa = 9.79YY35 pKa = 9.63SGARR39 pKa = 11.84NSRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84ISNITSIKK52 pKa = 9.97KK53 pKa = 9.2HH54 pKa = 6.5DD55 pKa = 3.62NMMPGFISPDD65 pKa = 2.82GTFNLNPLTTTTGFTSPFCPSARR88 pKa = 11.84RR89 pKa = 11.84LGLHH93 pKa = 6.85AEE95 pKa = 4.5GEE97 pKa = 4.66SARR100 pKa = 11.84QRR102 pKa = 11.84QTTFSVGYY110 pKa = 9.58KK111 pKa = 9.78EE112 pKa = 4.64RR113 pKa = 11.84LQIDD117 pKa = 3.77VLGDD121 pKa = 3.95GVWKK125 pKa = 9.08WRR127 pKa = 11.84RR128 pKa = 11.84VVFTYY133 pKa = 10.15KK134 pKa = 10.46GRR136 pKa = 11.84SLYY139 pKa = 10.86DD140 pKa = 3.27QDD142 pKa = 4.27TPWIVPYY149 pKa = 9.85YY150 pKa = 10.32NKK152 pKa = 9.93VVDD155 pKa = 4.11PTGHH159 pKa = 5.75GVDD162 pKa = 3.5MVRR165 pKa = 11.84AIAQPTLDD173 pKa = 3.35QAAHH177 pKa = 6.18IRR179 pKa = 11.84SVWWDD184 pKa = 3.37GFEE187 pKa = 4.25GGDD190 pKa = 3.11WGSEE194 pKa = 3.85FTAKK198 pKa = 10.5LDD200 pKa = 3.63TSRR203 pKa = 11.84ITPMYY208 pKa = 10.59DD209 pKa = 2.43RR210 pKa = 11.84TVTLNPGDD218 pKa = 3.87EE219 pKa = 4.48SGMSRR224 pKa = 11.84TFRR227 pKa = 11.84QWHH230 pKa = 5.8PARR233 pKa = 11.84KK234 pKa = 9.31NLVYY238 pKa = 10.8DD239 pKa = 4.54DD240 pKa = 6.05DD241 pKa = 5.44EE242 pKa = 7.64DD243 pKa = 4.03DD244 pKa = 3.79TAPTDD249 pKa = 3.52GGSPVSVTGKK259 pKa = 9.77PGMGDD264 pKa = 3.73LYY266 pKa = 11.33VYY268 pKa = 10.13DD269 pKa = 3.37IVYY272 pKa = 10.52NAVPATGG279 pKa = 3.29

Molecular weight: 31.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

784

195

310

261.3

29.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.888 ± 0.96

1.786 ± 0.619

7.908 ± 0.085

4.592 ± 0.921

3.444 ± 0.125

8.291 ± 0.305

3.189 ± 0.61

4.847 ± 0.563

3.061 ± 0.435

7.27 ± 1.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.531 ± 0.424

3.444 ± 0.201

5.995 ± 0.27

3.316 ± 0.353

9.311 ± 1.454

5.102 ± 0.753

6.888 ± 1.702

6.378 ± 0.187

3.061 ± 0.374

3.699 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski