Beihai tombus-like virus 10

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFX5|A0A1L3KFX5_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 10 OX=1922713 PE=4 SV=1
MM1 pKa = 6.94KK2 pKa = 9.84TVTINTKK9 pKa = 7.58WTADD13 pKa = 3.85SPDD16 pKa = 3.15SWLLNKK22 pKa = 9.92PYY24 pKa = 10.4RR25 pKa = 11.84IDD27 pKa = 3.49YY28 pKa = 8.45TEE30 pKa = 5.22KK31 pKa = 8.7IHH33 pKa = 7.02SVTVPALEE41 pKa = 4.26PEE43 pKa = 3.85ISIDD47 pKa = 3.43NVTTAEE53 pKa = 4.03YY54 pKa = 9.79MVGDD58 pKa = 4.13SSTNTMWPDD67 pKa = 3.2GPEE70 pKa = 3.45RR71 pKa = 11.84DD72 pKa = 3.89KK73 pKa = 11.18IYY75 pKa = 10.62VRR77 pKa = 11.84HH78 pKa = 6.11SFLGSINATQWLKK91 pKa = 9.49TATIVACYY99 pKa = 9.77SLSVKK104 pKa = 10.54YY105 pKa = 10.44KK106 pKa = 9.5ILQNGEE112 pKa = 4.07PNRR115 pKa = 11.84NGAGVFGRR123 pKa = 11.84DD124 pKa = 3.21LQLFRR129 pKa = 11.84HH130 pKa = 6.76DD131 pKa = 4.06LTVMATSPKK140 pKa = 10.29GITTAVVCPLVANTVVNVLDD160 pKa = 4.29DD161 pKa = 4.32RR162 pKa = 11.84VLQSLEE168 pKa = 3.98FKK170 pKa = 8.4TTFVVVAGWTYY181 pKa = 8.81TFVMHH186 pKa = 7.32SYY188 pKa = 8.92MRR190 pKa = 11.84PFFVNYY196 pKa = 9.29KK197 pKa = 9.69VEE199 pKa = 4.38AGTFIVFSAVATGAFMTYY217 pKa = 9.39IYY219 pKa = 10.11PGEE222 pKa = 4.26HH223 pKa = 5.14QVSLTT228 pKa = 3.42

Molecular weight: 25.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFN7|A0A1L3KFN7_9VIRU RNA-directed RNA polymerase OS=Beihai tombus-like virus 10 OX=1922713 PE=4 SV=1
MM1 pKa = 7.38TGRR4 pKa = 11.84GRR6 pKa = 11.84GRR8 pKa = 11.84GRR10 pKa = 11.84GRR12 pKa = 11.84GRR14 pKa = 11.84GRR16 pKa = 11.84GGRR19 pKa = 11.84RR20 pKa = 11.84APPQPRR26 pKa = 11.84PPPPQPPPPQASGRR40 pKa = 11.84QLARR44 pKa = 11.84RR45 pKa = 11.84MARR48 pKa = 11.84MSIGRR53 pKa = 11.84QMNTGKK59 pKa = 8.1TQIRR63 pKa = 11.84RR64 pKa = 11.84NEE66 pKa = 3.76LCIADD71 pKa = 3.98VTSKK75 pKa = 10.59FATAKK80 pKa = 9.88RR81 pKa = 11.84FRR83 pKa = 11.84MCPGHH88 pKa = 6.23TGMMLLDD95 pKa = 3.92AMGTIHH101 pKa = 7.44SSYY104 pKa = 10.05KK105 pKa = 7.66YY106 pKa = 9.52HH107 pKa = 6.09SVKK110 pKa = 10.23IHH112 pKa = 4.74VQGMGPTTSKK122 pKa = 10.91CILHH126 pKa = 6.63ICADD130 pKa = 3.56ASVGAGAVTPTAVLQTIPNATCRR153 pKa = 11.84AWQTTTMNIPSTILNRR169 pKa = 11.84YY170 pKa = 6.06NHH172 pKa = 6.64YY173 pKa = 10.14ISHH176 pKa = 7.01EE177 pKa = 4.12ATASDD182 pKa = 3.37QAEE185 pKa = 4.61PFTLIIIGAGSADD198 pKa = 3.59GDD200 pKa = 3.74DD201 pKa = 3.39VTFLVKK207 pKa = 10.5VNYY210 pKa = 10.16NVTFFNPEE218 pKa = 3.77PQQ220 pKa = 3.08

Molecular weight: 23.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1671

177

1046

417.8

46.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.54 ± 0.574

1.676 ± 0.231

4.728 ± 0.555

4.668 ± 0.751

3.291 ± 0.391

6.523 ± 0.901

3.052 ± 0.472

4.428 ± 0.57

6.882 ± 1.23

7.421 ± 1.11

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.232 ± 0.576

3.591 ± 0.552

6.942 ± 1.065

4.608 ± 1.405

6.403 ± 1.023

6.643 ± 0.611

6.942 ± 1.401

7.181 ± 1.252

0.958 ± 0.318

3.232 ± 0.66
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski