Scheffersomyces segobiensis virus L
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M4MEM7|M4MEM7_9VIRU Isoform of M4MG99 RNA-directed RNA polymerase (Fragment) OS=Scheffersomyces segobiensis virus L OX=1300323 GN=pol PE=3 SV=1
MM1 pKa = 7.85 SYY3 pKa = 10.93 QLFDD7 pKa = 3.66 QLTGPLANVLPNFKK21 pKa = 10.55 YY22 pKa = 9.93 LAKK25 pKa = 9.13 TVKK28 pKa = 10.32 YY29 pKa = 10.04 GIKK32 pKa = 10.05 ADD34 pKa = 3.41 VDD36 pKa = 3.31 IGVTSEE42 pKa = 3.62 FTNAYY47 pKa = 8.5 KK48 pKa = 10.55 RR49 pKa = 11.84 RR50 pKa = 11.84 GALFTNVLSPFGVFNAEE67 pKa = 4.27 VVRR70 pKa = 11.84 DD71 pKa = 3.8 PPIFDD76 pKa = 5.18 GINARR81 pKa = 11.84 FFNDD85 pKa = 2.91 YY86 pKa = 11.56 GEE88 pKa = 4.14 IDD90 pKa = 3.41 EE91 pKa = 4.6 RR92 pKa = 11.84 AIAEE96 pKa = 4.23 TLRR99 pKa = 11.84 LKK101 pKa = 9.56 GTYY104 pKa = 9.06 RR105 pKa = 11.84 PNVIAEE111 pKa = 4.65 LAPTIASCNSEE122 pKa = 3.75 NHH124 pKa = 5.34 VSVLVNLLRR133 pKa = 11.84 FSIIKK138 pKa = 10.46 RR139 pKa = 11.84 SDD141 pKa = 3.1 PKK143 pKa = 11.03 DD144 pKa = 3.64 FKK146 pKa = 11.07 FVNDD150 pKa = 4.16 DD151 pKa = 3.41 LFYY154 pKa = 11.14 DD155 pKa = 4.35 DD156 pKa = 4.02 GHH158 pKa = 7.31 LAVSYY163 pKa = 11.0 KK164 pKa = 10.84 NYY166 pKa = 10.59 LKK168 pKa = 10.82 RR169 pKa = 11.84 EE170 pKa = 4.09 FNEE173 pKa = 3.52 EE174 pKa = 3.6 LVFIFRR180 pKa = 11.84 EE181 pKa = 5.32 GIQIEE186 pKa = 4.4 RR187 pKa = 11.84 FSEE190 pKa = 4.3 STVVGDD196 pKa = 6.22 DD197 pKa = 4.42 FYY199 pKa = 11.75 CPNIEE204 pKa = 4.02 NLSARR209 pKa = 11.84 EE210 pKa = 3.84 FNILLTLLGSIKK222 pKa = 10.14 CDD224 pKa = 3.37 YY225 pKa = 10.06 PLRR228 pKa = 11.84 LAFSTPRR235 pKa = 11.84 LVDD238 pKa = 3.54 KK239 pKa = 11.01 LVLPLTTKK247 pKa = 9.58 HH248 pKa = 5.54 ASYY251 pKa = 11.2 YY252 pKa = 10.38 EE253 pKa = 4.05 VVNDD257 pKa = 3.82 FTANEE262 pKa = 3.73 VDD264 pKa = 3.68 QIIRR268 pKa = 11.84 KK269 pKa = 9.48 YY270 pKa = 9.76 IFANRR275 pKa = 11.84 VEE277 pKa = 4.39 TAFDD281 pKa = 3.65 LAYY284 pKa = 10.49 LIVVTAMFAPLPRR297 pKa = 11.84 AIEE300 pKa = 4.1 ANGWVSPVNLIKK312 pKa = 10.77 LPNVGSVRR320 pKa = 11.84 GLMPEE325 pKa = 3.91 MTGGQPLSKK334 pKa = 10.32 SPQKK338 pKa = 11.1 ALTWLNYY345 pKa = 8.51 KK346 pKa = 10.07 QSPGRR351 pKa = 11.84 IAIHH355 pKa = 7.13 AIAACEE361 pKa = 3.6 AYY363 pKa = 8.82 YY364 pKa = 10.45 TGFFEE369 pKa = 5.9 VLTASPNGIEE379 pKa = 4.42 DD380 pKa = 3.98 TLTAIGVSSSVTATPYY396 pKa = 11.59 KK397 pKa = 9.74 MFCEE401 pKa = 4.08 SAAYY405 pKa = 10.19 RR406 pKa = 11.84 FGKK409 pKa = 10.35 EE410 pKa = 4.46 FDD412 pKa = 5.32 LIWLTNAGVDD422 pKa = 4.14 CYY424 pKa = 11.54 SHH426 pKa = 7.26 LLATEE431 pKa = 4.39 PAALSILAKK440 pKa = 10.72 VVDD443 pKa = 4.39 TEE445 pKa = 4.4 LEE447 pKa = 4.4 GYY449 pKa = 9.94 DD450 pKa = 3.96 VYY452 pKa = 9.28 TTSAFGEE459 pKa = 4.46 DD460 pKa = 3.59 TVHH463 pKa = 7.31 VITKK467 pKa = 7.57 EE468 pKa = 3.95 VKK470 pKa = 8.6 PALFPVLSMGINDD483 pKa = 4.24 DD484 pKa = 4.18 RR485 pKa = 11.84 YY486 pKa = 9.19 FANSLEE492 pKa = 4.24 YY493 pKa = 10.7 SATLYY498 pKa = 10.83 ADD500 pKa = 3.19 HH501 pKa = 7.12 SEE503 pKa = 4.28 GVLATTEE510 pKa = 3.99 SDD512 pKa = 3.68 HH513 pKa = 6.18 MNKK516 pKa = 9.98 ALSILRR522 pKa = 11.84 IGGYY526 pKa = 9.84 DD527 pKa = 3.0 AVMTDD532 pKa = 3.38 RR533 pKa = 11.84 LSGLSYY539 pKa = 10.92 RR540 pKa = 11.84 NWAANSNGQVLPMLPPKK557 pKa = 10.67 LKK559 pKa = 10.67 GKK561 pKa = 7.74 STYY564 pKa = 10.36 VIPFNTIVKK573 pKa = 10.06 RR574 pKa = 11.84 KK575 pKa = 8.63 KK576 pKa = 9.55 SWMEE580 pKa = 3.76 LGSLTDD586 pKa = 3.83 SVTMTAKK593 pKa = 10.26 INLRR597 pKa = 11.84 GYY599 pKa = 11.24 VIMTNGKK606 pKa = 9.09 LVSTYY611 pKa = 10.29 LPKK614 pKa = 10.47 YY615 pKa = 7.52 QTINVYY621 pKa = 10.03 PKK623 pKa = 10.42 SLNKK627 pKa = 10.36 EE628 pKa = 3.7 EE629 pKa = 4.11 MVAVEE634 pKa = 4.74 MKK636 pKa = 10.44 TSATTIKK643 pKa = 10.23 YY644 pKa = 9.64 RR645 pKa = 11.84 YY646 pKa = 9.88 SGFLLAASQVEE657 pKa = 4.3 APPARR662 pKa = 11.84 LQLLSTDD669 pKa = 3.59 ILGSQPIEE677 pKa = 4.16 TSSEE681 pKa = 3.7 EE682 pKa = 3.93 AGAVGDD688 pKa = 4.16 DD689 pKa = 4.19 LEE691 pKa = 4.59 SVEE694 pKa = 4.41
Molecular weight: 77.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.19
IPC2_protein 5.321
IPC_protein 5.283
Toseland 5.27
ProMoST 5.372
Dawson 5.296
Bjellqvist 5.423
Wikipedia 5.181
Rodwell 5.219
Grimsley 5.219
Solomon 5.296
Lehninger 5.258
Nozaki 5.436
DTASelect 5.588
Thurlkill 5.296
EMBOSS 5.245
Sillero 5.512
Patrickios 3.91
IPC_peptide 5.308
IPC2_peptide 5.499
IPC2.peptide.svr19 5.535
Protein with the highest isoelectric point:
>tr|M4MEM7|M4MEM7_9VIRU Isoform of M4MG99 RNA-directed RNA polymerase (Fragment) OS=Scheffersomyces segobiensis virus L OX=1300323 GN=pol PE=3 SV=1
II1 pKa = 7.25 PVFGFFVGCEE11 pKa = 4.08 SSGSTTCPFTIIVYY25 pKa = 6.79 GHH27 pKa = 6.84 PWVAAHH33 pKa = 6.91 RR34 pKa = 11.84 NIEE37 pKa = 3.98 RR38 pKa = 11.84 RR39 pKa = 11.84 GWCSGRR45 pKa = 11.84 RR46 pKa = 11.84 LGEE49 pKa = 3.86 CRR51 pKa = 11.84 MSGLNYY57 pKa = 9.86 HH58 pKa = 6.43 NPVPLYY64 pKa = 10.4 VDD66 pKa = 3.53 EE67 pKa = 5.03 NMAGTVPEE75 pKa = 3.94 KK76 pKa = 10.46 SRR78 pKa = 11.84 YY79 pKa = 8.85 VLVHH83 pKa = 5.76 VSNDD87 pKa = 3.3 DD88 pKa = 3.91 VPVHH92 pKa = 6.36 NIDD95 pKa = 3.53 YY96 pKa = 10.72 PMLIPYY102 pKa = 9.62 CGEE105 pKa = 3.63 YY106 pKa = 10.39 TIGKK110 pKa = 9.01 RR111 pKa = 11.84 IKK113 pKa = 10.16 RR114 pKa = 11.84 RR115 pKa = 11.84 NIVCWYY121 pKa = 10.0 AKK123 pKa = 10.51 LDD125 pKa = 4.25 FISIYY130 pKa = 8.29 TPKK133 pKa = 10.57 LMSIVGGYY141 pKa = 9.64 ISGNIRR147 pKa = 11.84 SGMTVEE153 pKa = 4.02 NHH155 pKa = 6.3 RR156 pKa = 11.84 DD157 pKa = 3.23 FYY159 pKa = 11.27 MEE161 pKa = 3.84 FDD163 pKa = 3.22 KK164 pKa = 11.69 VIFNKK169 pKa = 8.5 VTSDD173 pKa = 3.88 EE174 pKa = 3.91 IRR176 pKa = 11.84 EE177 pKa = 4.02 LNEE180 pKa = 3.65 YY181 pKa = 9.64 VRR183 pKa = 11.84 SLDD186 pKa = 3.39 VTKK189 pKa = 9.86 ITGDD193 pKa = 2.74 HH194 pKa = 6.36 HH195 pKa = 7.13 LFVNRR200 pKa = 11.84 QEE202 pKa = 4.07 VDD204 pKa = 3.36 TLLEE208 pKa = 3.79 EE209 pKa = 4.44 AMNLGKK215 pKa = 11.01 YY216 pKa = 9.07 NDD218 pKa = 3.77 RR219 pKa = 11.84 LAALMVLKK227 pKa = 10.66 DD228 pKa = 3.34 VASRR232 pKa = 11.84 IGIDD236 pKa = 3.31 NRR238 pKa = 11.84 VSFITHH244 pKa = 6.54 LVYY247 pKa = 10.6 ILIAPPHH254 pKa = 6.24 AFVHH258 pKa = 6.04 LLSLIGEE265 pKa = 4.43 STDD268 pKa = 3.46 AEE270 pKa = 4.59 DD271 pKa = 3.59 YY272 pKa = 11.46 ASILKK277 pKa = 10.27 RR278 pKa = 11.84 EE279 pKa = 4.08 SGAAKK284 pKa = 10.0 QLQTVFRR291 pKa = 11.84 SDD293 pKa = 3.26 LASLYY298 pKa = 10.04 EE299 pKa = 4.17 LNVLVNRR306 pKa = 11.84 VDD308 pKa = 5.51 SVVDD312 pKa = 3.34 WNKK315 pKa = 10.87 EE316 pKa = 3.14 IDD318 pKa = 3.12 HH319 pKa = 6.17 RR320 pKa = 11.84 TNVKK324 pKa = 10.16 AVGISTEE331 pKa = 4.09 DD332 pKa = 3.39 VYY334 pKa = 11.4 KK335 pKa = 10.69 ISTEE339 pKa = 3.69 IFKK342 pKa = 10.92 DD343 pKa = 3.52 AVRR346 pKa = 11.84 EE347 pKa = 3.92 GRR349 pKa = 11.84 YY350 pKa = 7.69 PQRR353 pKa = 11.84 LEE355 pKa = 3.83 WQDD358 pKa = 3.05 YY359 pKa = 7.84 WVQRR363 pKa = 11.84 WSAMPNGSVISQYY376 pKa = 11.52 DD377 pKa = 3.28 EE378 pKa = 5.6 DD379 pKa = 3.94 IAIKK383 pKa = 10.52 KK384 pKa = 8.79 LLPFSARR391 pKa = 11.84 VKK393 pKa = 10.2 SAWFAAKK400 pKa = 9.63 TEE402 pKa = 4.34 SNYY405 pKa = 11.01 DD406 pKa = 3.27 YY407 pKa = 10.34 WLKK410 pKa = 9.87 RR411 pKa = 11.84 TPQIYY416 pKa = 10.24 ASTSTKK422 pKa = 10.45 YY423 pKa = 9.85 EE424 pKa = 3.76 WGKK427 pKa = 10.21 VRR429 pKa = 11.84 ALYY432 pKa = 10.68 GCDD435 pKa = 2.86 VTSFLHH441 pKa = 6.88 SDD443 pKa = 3.8 FGMTNCEE450 pKa = 4.99 DD451 pKa = 3.48 ILPSYY456 pKa = 10.42 FPVGRR461 pKa = 11.84 KK462 pKa = 9.44 ANEE465 pKa = 3.86 KK466 pKa = 9.51 YY467 pKa = 10.31 VRR469 pKa = 11.84 EE470 pKa = 4.27 SIEE473 pKa = 4.04 KK474 pKa = 9.79 FRR476 pKa = 11.84 EE477 pKa = 4.08 TVPVCFDD484 pKa = 3.45 YY485 pKa = 11.46 DD486 pKa = 3.86 DD487 pKa = 5.49 FNSQHH492 pKa = 5.86 STSSMRR498 pKa = 11.84 TVLQAWLDD506 pKa = 3.81 VNQKK510 pKa = 10.18 YY511 pKa = 7.77 LTEE514 pKa = 4.04 EE515 pKa = 3.91 QVNSVEE521 pKa = 4.13 WTRR524 pKa = 11.84 DD525 pKa = 3.41 SLDD528 pKa = 3.88 SILARR533 pKa = 11.84 FNSSGDD539 pKa = 3.8 TISLSGTLMSGWRR552 pKa = 11.84 LTSFMNTVLNRR563 pKa = 11.84 VYY565 pKa = 10.48 LVKK568 pKa = 11.04 ANLFKK573 pKa = 10.91 HH574 pKa = 6.23 LVYY577 pKa = 10.71 ALHH580 pKa = 6.7 NGDD583 pKa = 4.85 DD584 pKa = 3.93 MFGGAVNMAKK594 pKa = 10.15 AVAIIRR600 pKa = 11.84 DD601 pKa = 3.53 AKK603 pKa = 10.78 AIGVRR608 pKa = 11.84 AQVSKK613 pKa = 10.17 TNLGTIGEE621 pKa = 4.4 FLRR624 pKa = 11.84 VDD626 pKa = 3.56 TRR628 pKa = 11.84 AIDD631 pKa = 3.66 PTNAQYY637 pKa = 11.36 LSRR640 pKa = 11.84 SVATLVHH647 pKa = 5.98 GRR649 pKa = 11.84 VEE651 pKa = 4.35 SSAPNDD657 pKa = 3.29 LRR659 pKa = 11.84 EE660 pKa = 4.24 LVSAIITRR668 pKa = 11.84 SEE670 pKa = 3.91 EE671 pKa = 4.1 VISRR675 pKa = 11.84 GGDD678 pKa = 3.03 FRR680 pKa = 11.84 LINKK684 pKa = 7.75 LTQKK688 pKa = 9.4 TLDD691 pKa = 3.26 FAARR695 pKa = 11.84 LFNVDD700 pKa = 2.99 NTIIDD705 pKa = 3.92 TMLSNHH711 pKa = 6.32 PVQGGMNKK719 pKa = 9.88 DD720 pKa = 3.46 APVHH724 pKa = 5.48 LWRR727 pKa = 11.84 IEE729 pKa = 3.67 RR730 pKa = 11.84 RR731 pKa = 11.84 TEE733 pKa = 3.54 PFEE736 pKa = 5.75 RR737 pKa = 11.84 EE738 pKa = 3.8 TLLARR743 pKa = 11.84 YY744 pKa = 9.62 SLIAPGLFNYY754 pKa = 9.67 VDD756 pKa = 3.45 RR757 pKa = 11.84 VKK759 pKa = 10.86 EE760 pKa = 3.8 QFGVSEE766 pKa = 3.97 NLIDD770 pKa = 5.33 KK771 pKa = 11.01 EE772 pKa = 4.09 EE773 pKa = 4.0 LLYY776 pKa = 10.78 RR777 pKa = 11.84 AGVSLEE783 pKa = 3.71 KK784 pKa = 10.84 SRR786 pKa = 11.84 VYY788 pKa = 10.82 YY789 pKa = 10.23 EE790 pKa = 4.35 LVIEE794 pKa = 4.57 TTLGMDD800 pKa = 4.31 IYY802 pKa = 10.68 RR803 pKa = 11.84 ALHH806 pKa = 5.78 GVWKK810 pKa = 10.42 SPGYY814 pKa = 9.15 VAPIAKK820 pKa = 9.37 IRR822 pKa = 11.84 SLGLIPAKK830 pKa = 9.05 EE831 pKa = 3.9 LRR833 pKa = 11.84 NLNSVPAHH841 pKa = 7.24 LIRR844 pKa = 11.84 TARR847 pKa = 11.84 NPILMMGTIFF857 pKa = 4.87
Molecular weight: 97.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.715
IPC2_protein 6.942
IPC_protein 6.956
Toseland 6.956
ProMoST 7.395
Dawson 7.527
Bjellqvist 7.702
Wikipedia 7.483
Rodwell 7.541
Grimsley 7.088
Solomon 7.571
Lehninger 7.585
Nozaki 7.834
DTASelect 7.746
Thurlkill 7.79
EMBOSS 7.819
Sillero 7.951
Patrickios 4.266
IPC_peptide 7.571
IPC2_peptide 7.176
IPC2.peptide.svr19 7.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
1
3
3051
694
1500
1017.0
114.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.211 ± 0.445
0.983 ± 0.039
5.539 ± 0.13
5.965 ± 0.076
4.326 ± 0.211
5.637 ± 0.029
1.901 ± 0.262
6.391 ± 0.173
5.179 ± 0.148
9.407 ± 0.278
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.229 ± 0.068
5.375 ± 0.056
4.064 ± 0.284
1.803 ± 0.065
5.867 ± 0.593
7.407 ± 0.064
6.227 ± 0.265
8.325 ± 0.195
1.311 ± 0.208
4.851 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here