Enhydra lutris kenyoni

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Carnivora; Caniformia; Musteli

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32891 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Y9IE76|A0A2Y9IE76_ENHLU heat shock transcription factor X-linked OS=Enhydra lutris kenyoni OX=391180 GN=LOC111139124 PE=3 SV=1
MM1 pKa = 7.97DD2 pKa = 3.9VLFVAILAVQLILGQEE18 pKa = 4.16YY19 pKa = 10.36EE20 pKa = 4.23DD21 pKa = 3.72EE22 pKa = 4.97KK23 pKa = 11.49GLEE26 pKa = 3.95EE27 pKa = 4.88DD28 pKa = 5.13DD29 pKa = 4.47YY30 pKa = 11.5YY31 pKa = 11.42QVIYY35 pKa = 10.5YY36 pKa = 7.43YY37 pKa = 10.35TVTPNYY43 pKa = 10.56DD44 pKa = 3.23DD45 pKa = 4.83FGANFTVDD53 pKa = 3.11YY54 pKa = 11.54SMFEE58 pKa = 3.97SEE60 pKa = 5.5DD61 pKa = 3.28RR62 pKa = 11.84LNRR65 pKa = 11.84LDD67 pKa = 3.73KK68 pKa = 10.47EE69 pKa = 4.2VRR71 pKa = 11.84EE72 pKa = 4.17AAEE75 pKa = 4.06TTISHH80 pKa = 5.3EE81 pKa = 4.38TEE83 pKa = 4.08GADD86 pKa = 3.43TQKK89 pKa = 11.07PEE91 pKa = 4.21TVKK94 pKa = 10.79PMTTEE99 pKa = 3.86PQSPDD104 pKa = 3.21LNDD107 pKa = 3.7AVSSLQSPVPLLLSWALVQGGMYY130 pKa = 10.45FMM132 pKa = 6.15

Molecular weight: 15.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Y9KCE9|A0A2Y9KCE9_ENHLU survival motor neuron protein OS=Enhydra lutris kenyoni OX=391180 GN=LOC111155310 PE=4 SV=1
MM1 pKa = 7.57SSHH4 pKa = 4.97KK5 pKa = 8.81TFRR8 pKa = 11.84IRR10 pKa = 11.84RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.44QKK18 pKa = 8.59QNRR21 pKa = 11.84RR22 pKa = 11.84GAWNRR27 pKa = 11.84PIPQWFRR34 pKa = 11.84MKK36 pKa = 9.76TGNKK40 pKa = 8.57IRR42 pKa = 11.84YY43 pKa = 7.09NSKK46 pKa = 8.3RR47 pKa = 11.84RR48 pKa = 11.84HH49 pKa = 3.95WRR51 pKa = 11.84RR52 pKa = 11.84TKK54 pKa = 10.83LGLL57 pKa = 3.67

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19196

13695

32891

20466548

32

34347

622.3

69.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.116 ± 0.014

2.164 ± 0.01

4.844 ± 0.009

7.15 ± 0.017

3.584 ± 0.007

6.604 ± 0.016

2.58 ± 0.007

4.224 ± 0.012

5.657 ± 0.018

9.936 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.13 ± 0.006

3.519 ± 0.009

6.447 ± 0.021

4.784 ± 0.011

5.845 ± 0.011

8.416 ± 0.016

5.215 ± 0.01

6.014 ± 0.012

1.169 ± 0.004

2.591 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski