Paenibacillus nanensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5258 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A1UTI4|A0A3A1UTI4_9BACL Uncharacterized protein OS=Paenibacillus nanensis OX=393251 GN=D3P08_15390 PE=4 SV=1
MM1 pKa = 7.95RR2 pKa = 11.84KK3 pKa = 7.21QTKK6 pKa = 9.59KK7 pKa = 10.09PFSIVMALLLTLTLTMSSFVYY28 pKa = 10.75AEE30 pKa = 3.99GLGDD34 pKa = 3.84EE35 pKa = 4.5EE36 pKa = 5.01KK37 pKa = 9.95KK38 pKa = 9.25TVGLQGEE45 pKa = 4.26PAGASLAGEE54 pKa = 4.03AHH56 pKa = 6.57AASDD60 pKa = 3.86EE61 pKa = 4.12QTEE64 pKa = 4.45ASCTTYY70 pKa = 11.05YY71 pKa = 11.33GFADD75 pKa = 4.49KK76 pKa = 10.6LAQQDD81 pKa = 3.52PTVPYY86 pKa = 9.22ITINGDD92 pKa = 3.05GTATTKK98 pKa = 10.66FKK100 pKa = 8.74TTQDD104 pKa = 3.33CVQASFSVYY113 pKa = 9.82TYY115 pKa = 11.06GEE117 pKa = 4.16SPTPYY122 pKa = 9.67EE123 pKa = 3.84NQVYY127 pKa = 10.17YY128 pKa = 10.77SGEE131 pKa = 3.73TAQYY135 pKa = 10.63DD136 pKa = 3.51KK137 pKa = 11.51AGNYY141 pKa = 7.98EE142 pKa = 4.21LTISIPVCGNSQIDD156 pKa = 4.49LYY158 pKa = 11.02GGPIQTTLGPGGHH171 pKa = 6.62KK172 pKa = 10.19PGTYY176 pKa = 9.3RR177 pKa = 11.84SYY179 pKa = 11.52LYY181 pKa = 9.91IIKK184 pKa = 10.02SPCPTPTPTDD194 pKa = 3.97PPTEE198 pKa = 4.41TPTTTPTDD206 pKa = 4.05PPTEE210 pKa = 4.41TPTTTPTDD218 pKa = 4.05PPTEE222 pKa = 4.41TPTTTPTDD230 pKa = 4.05PPTEE234 pKa = 4.41TPTTTPTDD242 pKa = 4.05PPTEE246 pKa = 4.39TPTTTPSDD254 pKa = 4.17PPTEE258 pKa = 4.47TPTTTPTDD266 pKa = 4.05PPTEE270 pKa = 4.41TPTTTPTDD278 pKa = 4.05PPTEE282 pKa = 4.39TPTTTPSDD290 pKa = 4.17PPTEE294 pKa = 4.47TPTTTPTDD302 pKa = 4.05PPTEE306 pKa = 4.41TPTTTPTDD314 pKa = 4.05PPTEE318 pKa = 4.39TPTTTPSDD326 pKa = 4.17PPTEE330 pKa = 4.45TPTTTPSDD338 pKa = 4.17PPTEE342 pKa = 4.45TPTTTPSDD350 pKa = 4.17PPTEE354 pKa = 4.47TPTTTPTQTPTSTPTQTPTSTPSWTPTPPPTWTWTPTPTPTPTTTPTATPTSTPTATPTSTPTDD418 pKa = 4.05PPTEE422 pKa = 4.47SPTPTPTDD430 pKa = 3.54TPTEE434 pKa = 4.36APSPTPTATTIITEE448 pKa = 4.24EE449 pKa = 4.31TPPDD453 pKa = 3.89DD454 pKa = 4.64GGTGGEE460 pKa = 4.08TDD462 pKa = 3.87GDD464 pKa = 3.88EE465 pKa = 4.21GSIPIDD471 pKa = 3.54SLPQTGDD478 pKa = 3.4SSPMPYY484 pKa = 10.42YY485 pKa = 11.19LLGTFIAVSGIVALRR500 pKa = 11.84KK501 pKa = 9.33RR502 pKa = 11.84RR503 pKa = 11.84RR504 pKa = 11.84QPP506 pKa = 2.87

Molecular weight: 53.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A1URQ4|A0A3A1URQ4_9BACL DUF115 domain-containing protein OS=Paenibacillus nanensis OX=393251 GN=D3P08_25120 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.84PNVSKK11 pKa = 10.73RR12 pKa = 11.84AKK14 pKa = 8.72VHH16 pKa = 5.43GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5258

0

5258

1792965

26

3082

341.0

37.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.076 ± 0.044

0.698 ± 0.011

5.159 ± 0.026

7.003 ± 0.038

4.09 ± 0.026

7.674 ± 0.031

1.999 ± 0.014

6.372 ± 0.031

5.067 ± 0.025

10.024 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.818 ± 0.017

3.642 ± 0.028

4.093 ± 0.021

3.566 ± 0.021

5.281 ± 0.037

6.286 ± 0.025

5.308 ± 0.033

6.982 ± 0.028

1.338 ± 0.015

3.523 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski