Streptococcus satellite phage Javan608

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZRY6|A0A4D5ZRY6_9VIRU DNA-binding protein OS=Streptococcus satellite phage Javan608 OX=2558777 GN=JavanS608_0012 PE=4 SV=1
MM1 pKa = 7.26TLKK4 pKa = 10.6KK5 pKa = 9.51HH6 pKa = 5.8TDD8 pKa = 2.91NARR11 pKa = 11.84AFTFRR16 pKa = 11.84HH17 pKa = 5.02EE18 pKa = 4.5FEE20 pKa = 4.78TVDD23 pKa = 3.51HH24 pKa = 6.86ANVTSTAILGYY35 pKa = 9.38MVGTYY40 pKa = 7.09EE41 pKa = 5.11QPTIDD46 pKa = 3.06ITISKK51 pKa = 10.49NEE53 pKa = 3.98ANNAYY58 pKa = 9.29TMLIDD63 pKa = 3.91YY64 pKa = 10.76VSDD67 pKa = 3.93SNLSEE72 pKa = 4.11PFNRR76 pKa = 11.84VCDD79 pKa = 3.87SFEE82 pKa = 4.57GYY84 pKa = 10.58SKK86 pKa = 11.14GCSEE90 pKa = 4.5AA91 pKa = 3.97

Molecular weight: 10.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZWW6|A0A4D5ZWW6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan608 OX=2558777 GN=JavanS608_0009 PE=4 SV=1
MM1 pKa = 7.78KK2 pKa = 10.26INEE5 pKa = 4.22IKK7 pKa = 10.67KK8 pKa = 9.76KK9 pKa = 10.54DD10 pKa = 3.3GSTVYY15 pKa = 10.36RR16 pKa = 11.84ANIYY20 pKa = 10.62LGVDD24 pKa = 3.95VITGKK29 pKa = 10.74KK30 pKa = 7.55VTTKK34 pKa = 9.32VTARR38 pKa = 11.84TKK40 pKa = 10.91KK41 pKa = 9.48EE42 pKa = 4.24LKK44 pKa = 9.67TKK46 pKa = 10.1AQQAQFDD53 pKa = 4.54FKK55 pKa = 11.59ANGSTRR61 pKa = 11.84YY62 pKa = 9.72KK63 pKa = 9.99EE64 pKa = 4.21VAIEE68 pKa = 4.25TYY70 pKa = 10.63EE71 pKa = 3.95EE72 pKa = 4.45LAISWWDD79 pKa = 3.38SYY81 pKa = 11.84KK82 pKa = 10.19NTVKK86 pKa = 10.95ANTKK90 pKa = 9.64RR91 pKa = 11.84AQKK94 pKa = 10.81GLLNNHH100 pKa = 5.72VLPLFGEE107 pKa = 4.67FKK109 pKa = 10.56LDD111 pKa = 3.59KK112 pKa = 10.06LTTPLIQSTMNKK124 pKa = 9.46LANSTNTGEE133 pKa = 4.13VGAYY137 pKa = 8.95LHH139 pKa = 6.49YY140 pKa = 11.1DD141 pKa = 3.92KK142 pKa = 11.05IHH144 pKa = 6.58ALNKK148 pKa = 10.18RR149 pKa = 11.84ILQYY153 pKa = 11.17GVVLQVIPTNPASNVVLPRR172 pKa = 11.84NTQKK176 pKa = 11.1DD177 pKa = 3.22KK178 pKa = 10.56KK179 pKa = 10.89AKK181 pKa = 9.81VKK183 pKa = 10.6HH184 pKa = 5.76FNNDD188 pKa = 2.8EE189 pKa = 4.08LKK191 pKa = 10.82QFLTYY196 pKa = 10.53LDD198 pKa = 4.07SLDD201 pKa = 3.34NSKK204 pKa = 11.04YY205 pKa = 10.38KK206 pKa = 10.64NYY208 pKa = 10.65YY209 pKa = 10.47DD210 pKa = 3.03ITLYY214 pKa = 10.98KK215 pKa = 10.3FLLATGCRR223 pKa = 11.84INEE226 pKa = 4.0ALALSWSDD234 pKa = 2.86IDD236 pKa = 4.49LDD238 pKa = 3.79NSVVHH243 pKa = 5.57ITKK246 pKa = 9.23TLNYY250 pKa = 9.33EE251 pKa = 4.08MKK253 pKa = 10.78LNSPKK258 pKa = 10.55SKK260 pKa = 10.22SGYY263 pKa = 9.62RR264 pKa = 11.84DD265 pKa = 2.83IDD267 pKa = 3.15IDD269 pKa = 3.87KK270 pKa = 9.04QTVTMMKK277 pKa = 9.91RR278 pKa = 11.84YY279 pKa = 7.4QHH281 pKa = 6.34KK282 pKa = 8.59QTQEE286 pKa = 3.08AWKK289 pKa = 10.12LGRR292 pKa = 11.84TEE294 pKa = 4.07TVVFSDD300 pKa = 6.21FIHH303 pKa = 7.08EE304 pKa = 4.36YY305 pKa = 9.57PNSRR309 pKa = 11.84TLQSRR314 pKa = 11.84LGTHH318 pKa = 6.83FKK320 pKa = 10.69RR321 pKa = 11.84AGVTNIGFHH330 pKa = 6.07GFRR333 pKa = 11.84HH334 pKa = 4.87THH336 pKa = 6.58ASLLLNSGIPYY347 pKa = 10.0KK348 pKa = 10.34EE349 pKa = 3.8LQHH352 pKa = 6.88RR353 pKa = 11.84LGHH356 pKa = 6.17SKK358 pKa = 11.08LSMTMDD364 pKa = 3.56IYY366 pKa = 11.68SHH368 pKa = 6.98LSKK371 pKa = 11.0EE372 pKa = 4.25NAKK375 pKa = 10.37KK376 pKa = 10.27AVSFYY381 pKa = 10.95EE382 pKa = 4.17VALKK386 pKa = 10.72SII388 pKa = 4.28

Molecular weight: 44.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

2018

53

388

144.1

16.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.104 ± 0.437

0.545 ± 0.126

6.293 ± 0.603

6.987 ± 0.782

3.717 ± 0.343

5.302 ± 0.414

1.833 ± 0.342

6.392 ± 0.644

9.713 ± 0.673

9.465 ± 0.639

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.329 ± 0.367

5.897 ± 0.538

3.023 ± 0.645

3.915 ± 0.547

4.609 ± 0.451

6.194 ± 0.592

6.739 ± 0.605

5.897 ± 0.281

0.892 ± 0.255

5.154 ± 0.327
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski