Streptococcus phage PH10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5J9A1|C5J9A1_9CAUD Uncharacterized protein OS=Streptococcus phage PH10 OX=644007 PE=4 SV=1
MM1 pKa = 7.4NYY3 pKa = 10.16KK4 pKa = 8.92VTVDD8 pKa = 3.47GKK10 pKa = 9.6EE11 pKa = 3.67IEE13 pKa = 4.34YY14 pKa = 10.41GALVEE19 pKa = 4.45KK20 pKa = 10.94SRR22 pKa = 11.84FSEE25 pKa = 4.45KK26 pKa = 9.78EE27 pKa = 3.15WSAIYY32 pKa = 10.73AEE34 pKa = 4.38IVKK37 pKa = 10.19QNQPEE42 pKa = 4.17VFEE45 pKa = 4.5SKK47 pKa = 10.63KK48 pKa = 10.94ADD50 pKa = 3.31TDD52 pKa = 4.19YY53 pKa = 11.43IDD55 pKa = 3.74TLGAMIALEE64 pKa = 4.05EE65 pKa = 4.29RR66 pKa = 11.84YY67 pKa = 9.29EE68 pKa = 4.12ALLEE72 pKa = 4.91LLPQDD77 pKa = 3.46QFSYY81 pKa = 11.1AGTHH85 pKa = 5.91PKK87 pKa = 9.09WVADD91 pKa = 3.73AVAEE95 pKa = 4.06NTLNKK100 pKa = 10.07EE101 pKa = 3.94DD102 pKa = 4.74TIDD105 pKa = 4.76DD106 pKa = 3.44ICDD109 pKa = 3.48FLEE112 pKa = 4.48RR113 pKa = 11.84CSTLQQLQDD122 pKa = 3.18EE123 pKa = 4.39LMEE126 pKa = 4.13YY127 pKa = 10.82FNLEE131 pKa = 3.91EE132 pKa = 4.2SS133 pKa = 3.68

Molecular weight: 15.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5J953|C5J953_9CAUD Integrase OS=Streptococcus phage PH10 OX=644007 PE=3 SV=1
MM1 pKa = 7.41NKK3 pKa = 9.86EE4 pKa = 3.28DD5 pKa = 3.75WLRR8 pKa = 11.84LLEE11 pKa = 4.17KK12 pKa = 10.49AIDD15 pKa = 4.38NIPEE19 pKa = 4.14TVTAIASLVTAITVARR35 pKa = 11.84QNKK38 pKa = 7.97KK39 pKa = 9.77RR40 pKa = 11.84KK41 pKa = 9.0PKK43 pKa = 9.83FRR45 pKa = 11.84QKK47 pKa = 10.77KK48 pKa = 8.18RR49 pKa = 3.24

Molecular weight: 5.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

9673

24

1137

179.1

20.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.523 ± 0.426

0.589 ± 0.128

6.037 ± 0.324

8.074 ± 0.464

4.177 ± 0.279

5.924 ± 0.385

1.427 ± 0.173

7.547 ± 0.312

8.374 ± 0.364

8.787 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.429 ± 0.237

5.893 ± 0.278

2.791 ± 0.255

4.197 ± 0.271

4.218 ± 0.321

6.13 ± 0.398

5.727 ± 0.408

6.203 ± 0.328

1.272 ± 0.163

3.68 ± 0.369
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski