Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Nostoc;

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6071 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8YRM1|Q8YRM1_NOSS1 Alr3425 protein OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=alr3425 PE=4 SV=1
MM1 pKa = 6.93GTYY4 pKa = 10.47NGTTPFPDD12 pKa = 4.36PGSSTSTALDD22 pKa = 3.24TGLLIGGSLLSTPSLFQDD40 pKa = 3.22NLSRR44 pKa = 11.84LIPSDD49 pKa = 3.1LSDD52 pKa = 3.65YY53 pKa = 11.38YY54 pKa = 11.22RR55 pKa = 11.84FTLSSSSIVTLEE67 pKa = 4.73LDD69 pKa = 3.6GLTDD73 pKa = 3.66NVNLFLRR80 pKa = 11.84NSAGSSLAASSNAGTTAEE98 pKa = 4.69RR99 pKa = 11.84IRR101 pKa = 11.84FSISDD106 pKa = 3.63PPDD109 pKa = 3.24NPFYY113 pKa = 11.12AHH115 pKa = 7.26AFIPSGSISTPYY127 pKa = 10.66DD128 pKa = 3.18LRR130 pKa = 11.84LSAEE134 pKa = 4.44VIQQNNINDD143 pKa = 3.59NTIANARR150 pKa = 11.84NIGTFNTTTSITPVTDD166 pKa = 3.5YY167 pKa = 11.44VSGSGQVIDD176 pKa = 3.7QNDD179 pKa = 4.33YY180 pKa = 11.67YY181 pKa = 11.63SFTLTNSGTVDD192 pKa = 3.0INLNSLNGTDD202 pKa = 3.52TLYY205 pKa = 11.39ADD207 pKa = 4.34LQLISSSNSVIQTSATVGTSLEE229 pKa = 4.34SISRR233 pKa = 11.84SLAAGTYY240 pKa = 8.71YY241 pKa = 9.95IRR243 pKa = 11.84AYY245 pKa = 10.13SQSDD249 pKa = 3.24PGNYY253 pKa = 9.33NLEE256 pKa = 4.6FNFSADD262 pKa = 3.62PPDD265 pKa = 4.16AGGNTSDD272 pKa = 3.27TSTPINLPATFSEE285 pKa = 4.62IISDD289 pKa = 3.87QVSLGDD295 pKa = 3.65SSDD298 pKa = 3.97YY299 pKa = 11.15YY300 pKa = 11.43QFTLASASLVEE311 pKa = 4.18IQFTSLTADD320 pKa = 3.11ANLYY324 pKa = 10.34LQTQNGGNILSSTQPGTALDD344 pKa = 3.89AVRR347 pKa = 11.84LSLNAGTYY355 pKa = 10.33NILVNRR361 pKa = 11.84GSTEE365 pKa = 3.79TAQYY369 pKa = 9.0TLSGFAQAIGNDD381 pKa = 3.46QAPNSTTLALNLNLNSPISLNEE403 pKa = 3.54FVGNIDD409 pKa = 3.31TNDD412 pKa = 3.38YY413 pKa = 11.53YY414 pKa = 11.56KK415 pKa = 10.39FTVNGTTEE423 pKa = 3.86INLDD427 pKa = 3.64LSILNSYY434 pKa = 10.48LLDD437 pKa = 4.15PQLVNADD444 pKa = 3.69VQILNSGGTQVAISNQTGNSNEE466 pKa = 4.42SINTILGAGTYY477 pKa = 9.13FIRR480 pKa = 11.84VYY482 pKa = 10.62TSGLANTFYY491 pKa = 11.07DD492 pKa = 4.8LNITAQSQALLVQDD506 pKa = 4.55INPTGNSDD514 pKa = 3.7PANLTTLGNTLYY526 pKa = 9.07FTANDD531 pKa = 5.27GINGVQLWSSNGDD544 pKa = 2.67ITTRR548 pKa = 11.84LSNISSFNPTNLIVFNNRR566 pKa = 11.84LYY568 pKa = 10.66FAASNDD574 pKa = 3.55TFGRR578 pKa = 11.84EE579 pKa = 3.4LWEE582 pKa = 4.21YY583 pKa = 11.43NGTTVNRR590 pKa = 11.84ISDD593 pKa = 3.77INVGAGNSNPGNLTVAGNKK612 pKa = 10.0LFFTAVDD619 pKa = 3.32NDD621 pKa = 4.56SIRR624 pKa = 11.84KK625 pKa = 7.89LWVYY629 pKa = 11.14NGTNVNLVDD638 pKa = 4.0VNASFSNSSTPTFTTTFNNQLFFTAQNNSQLWSTDD673 pKa = 2.65GTIGGTQVISAGGITKK689 pKa = 8.0STPRR693 pKa = 11.84NLTVVGNTLYY703 pKa = 9.09FTANNGTSGHH713 pKa = 6.36EE714 pKa = 3.17IWQYY718 pKa = 11.25QNGTTASLLEE728 pKa = 5.61DD729 pKa = 3.28ITPGNNSFAPSSLTAVGNTLYY750 pKa = 10.7FVTDD754 pKa = 3.26SDD756 pKa = 3.98NDD758 pKa = 3.59FNLEE762 pKa = 3.89LWKK765 pKa = 10.77SDD767 pKa = 2.9GTAAGTDD774 pKa = 3.48IIGTDD779 pKa = 3.52GQAPNLGLGSFSLTAVDD796 pKa = 3.25NTLYY800 pKa = 10.55FVANDD805 pKa = 4.7PISGLEE811 pKa = 3.75LWKK814 pKa = 9.82TDD816 pKa = 3.05GTFANTAVVRR826 pKa = 11.84NISSGAADD834 pKa = 3.89SLPTGLVNFNGTLAFAASDD853 pKa = 4.42GINRR857 pKa = 11.84EE858 pKa = 3.93VWFSNGTEE866 pKa = 3.95LNTRR870 pKa = 11.84KK871 pKa = 9.98VSNINPTGNANPASLTVVGTKK892 pKa = 10.57LFFTANGANGTEE904 pKa = 4.5LYY906 pKa = 10.85VII908 pKa = 4.86

Molecular weight: 96.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8YS16|Q8YS16_NOSS1 Alr3276 protein OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=alr3276 PE=4 SV=1
MM1 pKa = 7.08QRR3 pKa = 11.84TLGGTNRR10 pKa = 11.84KK11 pKa = 9.1RR12 pKa = 11.84KK13 pKa = 7.65RR14 pKa = 11.84TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TPDD26 pKa = 3.17GRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.83RR37 pKa = 11.84GRR39 pKa = 11.84HH40 pKa = 4.78RR41 pKa = 11.84LSVV44 pKa = 3.19

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6070

1

6071

1955973

17

4936

322.2

35.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.998 ± 0.034

0.952 ± 0.013

4.81 ± 0.026

6.109 ± 0.034

3.948 ± 0.02

6.627 ± 0.043

1.832 ± 0.02

6.947 ± 0.026

4.786 ± 0.034

10.966 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.777 ± 0.016

4.635 ± 0.034

4.659 ± 0.024

5.555 ± 0.033

5.011 ± 0.031

6.43 ± 0.027

5.842 ± 0.037

6.619 ± 0.029

1.427 ± 0.012

3.069 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski