Cyanobacterium stanieri (strain ATCC 29140 / PCC 7202)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Geminocystaceae; Cyanobacterium; Cyanobacterium stanieri

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2831 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9YM21|K9YM21_CYASC Anti-sigma factor antagonist OS=Cyanobacterium stanieri (strain ATCC 29140 / PCC 7202) OX=292563 GN=Cyast_1477 PE=3 SV=1
MM1 pKa = 7.47NNLLFKK7 pKa = 10.53IEE9 pKa = 4.86EE10 pKa = 4.15YY11 pKa = 11.1SKK13 pKa = 11.29DD14 pKa = 3.57NPQLVVVVQAQDD26 pKa = 2.86NGQNLEE32 pKa = 3.71IMTFRR37 pKa = 11.84GFSSNLTGATEE48 pKa = 4.23YY49 pKa = 11.33DD50 pKa = 3.34PDD52 pKa = 4.53LPVLSSSGIILSVDD66 pKa = 4.04LLQAPYY72 pKa = 11.1NPVNPQYY79 pKa = 10.39IQQNLTPAQFEE90 pKa = 4.93ANFLSS95 pKa = 3.86

Molecular weight: 10.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9YIE8|K9YIE8_CYASC Uncharacterized protein OS=Cyanobacterium stanieri (strain ATCC 29140 / PCC 7202) OX=292563 GN=Cyast_0267 PE=4 SV=1
MM1 pKa = 7.64SKK3 pKa = 8.22HH4 pKa = 5.3TLNGTRR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 9.42QKK14 pKa = 8.48RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TKK26 pKa = 10.59DD27 pKa = 3.35GRR29 pKa = 11.84KK30 pKa = 9.05VIQARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.02GRR40 pKa = 11.84QRR42 pKa = 11.84LAVV45 pKa = 3.51

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2831

0

2831

902738

29

2456

318.9

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.761 ± 0.049

0.997 ± 0.017

5.29 ± 0.032

6.673 ± 0.043

4.217 ± 0.038

6.597 ± 0.049

1.86 ± 0.023

7.899 ± 0.046

5.687 ± 0.048

10.613 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.038 ± 0.021

5.343 ± 0.052

4.307 ± 0.035

4.786 ± 0.034

4.484 ± 0.036

6.222 ± 0.034

5.352 ± 0.031

6.272 ± 0.037

1.317 ± 0.022

3.286 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski