Thaumarchaeota archaeon SCGC AB-539-E09
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 646 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M7TBZ5|M7TBZ5_9ARCH Nitrate/nitrite transporter OS=Thaumarchaeota archaeon SCGC AB-539-E09 OX=1198115 GN=MCGE09_00155 PE=4 SV=1
MM1 pKa = 7.27 VLLPTCGSLFPDD13 pKa = 3.71 EE14 pKa = 4.69 EE15 pKa = 4.42 MSLKK19 pKa = 10.13 RR20 pKa = 11.84 QYY22 pKa = 10.63 IYY24 pKa = 11.3 VVAICLSVIVAGAIIAYY41 pKa = 10.14 NPFQEE46 pKa = 5.28 KK47 pKa = 10.45 EE48 pKa = 3.85 DD49 pKa = 4.19 DD50 pKa = 3.89 NVFKK54 pKa = 10.55 FPAGIPPSLGMEE66 pKa = 4.24 YY67 pKa = 9.35 ATTLGEE73 pKa = 4.11 GDD75 pKa = 3.74 VNSIQVSGQGSVSAKK90 pKa = 8.94 ATQATLTLGAWTEE103 pKa = 4.18 DD104 pKa = 3.41 PVAAEE109 pKa = 3.9 AVEE112 pKa = 4.91 DD113 pKa = 3.84 NALLMTSIIDD123 pKa = 4.24 AIMGLGIDD131 pKa = 3.65 EE132 pKa = 5.3 DD133 pKa = 4.22 KK134 pKa = 11.03 IKK136 pKa = 10.05 TVTYY140 pKa = 10.27 SVSTNYY146 pKa = 10.31 DD147 pKa = 2.77 WEE149 pKa = 4.2 TRR151 pKa = 11.84 TIKK154 pKa = 10.55 GYY156 pKa = 10.54 RR157 pKa = 11.84 VTNMIQVVVEE167 pKa = 4.77 DD168 pKa = 3.51 IDD170 pKa = 3.88 MVGEE174 pKa = 4.48 VIDD177 pKa = 3.56 QAAAEE182 pKa = 4.46 GANSIQGISFGISDD196 pKa = 4.5 EE197 pKa = 4.24 EE198 pKa = 4.41 AKK200 pKa = 10.04 TLANDD205 pKa = 4.17 AYY207 pKa = 11.3 VLALQNAQGKK217 pKa = 9.94 ADD219 pKa = 5.66 LIASTLDD226 pKa = 3.5 LEE228 pKa = 4.65 ITGVLYY234 pKa = 10.87 VSEE237 pKa = 4.5 SSYY240 pKa = 11.19 TPYY243 pKa = 10.73 SPYY246 pKa = 10.25 RR247 pKa = 11.84 SYY249 pKa = 11.7 AEE251 pKa = 3.92 EE252 pKa = 4.78 AMDD255 pKa = 4.67 SGFAAPTPIIEE266 pKa = 4.49 GTLSVSVSVQVTFSFQQ282 pKa = 2.9
Molecular weight: 30.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.859
IPC_protein 3.834
Toseland 3.643
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.706
Rodwell 3.668
Grimsley 3.554
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.101
Thurlkill 3.681
EMBOSS 3.719
Sillero 3.948
Patrickios 1.125
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|M7TAJ0|M7TAJ0_9ARCH Extracellular solute-binding protein family 5 (Fragment) OS=Thaumarchaeota archaeon SCGC AB-539-E09 OX=1198115 GN=MCGE09_00563 PE=4 SV=1
MM1 pKa = 7.71 RR2 pKa = 11.84 LCSRR6 pKa = 11.84 EE7 pKa = 3.74 IGVGFSAFRR16 pKa = 11.84 MLTGYY21 pKa = 11.15 LNMPGRR27 pKa = 11.84 TLGYY31 pKa = 10.01 RR32 pKa = 11.84 GIKK35 pKa = 9.74 KK36 pKa = 9.45 EE37 pKa = 3.77 RR38 pKa = 11.84 LYY40 pKa = 9.77 MHH42 pKa = 7.19 KK43 pKa = 10.51 FFFNCC48 pKa = 4.15
Molecular weight: 5.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.794
IPC_protein 10.482
Toseland 10.365
ProMoST 10.131
Dawson 10.555
Bjellqvist 10.292
Wikipedia 10.774
Rodwell 10.789
Grimsley 10.628
Solomon 10.628
Lehninger 10.584
Nozaki 10.394
DTASelect 10.277
Thurlkill 10.409
EMBOSS 10.76
Sillero 10.482
Patrickios 10.599
IPC_peptide 10.613
IPC2_peptide 9.487
IPC2.peptide.svr19 8.348
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
646
0
646
143127
17
1365
221.6
25.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.682 ± 0.11
1.029 ± 0.034
5.522 ± 0.087
7.265 ± 0.131
4.258 ± 0.091
6.956 ± 0.102
1.856 ± 0.048
8.091 ± 0.12
6.354 ± 0.114
9.712 ± 0.105
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.692 ± 0.058
4.416 ± 0.097
4.193 ± 0.078
2.502 ± 0.056
5.252 ± 0.105
6.621 ± 0.085
5.238 ± 0.084
7.01 ± 0.102
1.348 ± 0.044
4.0 ± 0.08
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here