Carp edema virus
Average proteome isoelectric point is 5.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B7QBP8|A0A5B7QBP8_9POXV Major core protein 4a precursor OS=Carp edema virus OX=1653087 GN=P4a PE=3 SV=1
MM1 pKa = 7.1 VATAAARR8 pKa = 11.84 DD9 pKa = 3.76 PEE11 pKa = 4.63 LRR13 pKa = 11.84 DD14 pKa = 3.37 SNYY17 pKa = 10.35 VMAAPAANPFANMVKK32 pKa = 10.26 FDD34 pKa = 4.47 IPDD37 pKa = 3.7 IYY39 pKa = 11.05 QFAEE43 pKa = 3.93 IGLQQIKK50 pKa = 7.88 MTTLHH55 pKa = 6.06 YY56 pKa = 10.66 CDD58 pKa = 4.84 FVSKK62 pKa = 10.46 VLPHH66 pKa = 6.55 PNYY69 pKa = 9.99 VVKK72 pKa = 10.72 KK73 pKa = 10.12 DD74 pKa = 3.12 IGYY77 pKa = 10.17 VDD79 pKa = 4.9 TIPEE83 pKa = 3.92 QVKK86 pKa = 9.9 IANAMFWKK94 pKa = 10.43 GIQIDD99 pKa = 4.22 DD100 pKa = 3.85 WNKK103 pKa = 9.57 MNVFDD108 pKa = 5.49 KK109 pKa = 11.3 TMTSNLALVLGLYY122 pKa = 10.72 DD123 pKa = 3.25 EE124 pKa = 4.61 TVYY127 pKa = 10.83 FKK129 pKa = 11.41 NSTNKK134 pKa = 9.92 FEE136 pKa = 4.3 ITFCGILDD144 pKa = 3.95 QIPQGVAVKK153 pKa = 9.74 RR154 pKa = 11.84 VCFLPYY160 pKa = 10.48 KK161 pKa = 10.2 LGNATIYY168 pKa = 8.5 KK169 pKa = 7.62 TLKK172 pKa = 10.34 HH173 pKa = 6.25 VSTQIWEE180 pKa = 4.14 GFYY183 pKa = 11.28 ANGNLITNIKK193 pKa = 9.57 QLQKK197 pKa = 9.68 VTNDD201 pKa = 3.13 EE202 pKa = 4.34 KK203 pKa = 10.87 KK204 pKa = 9.05 TLGLTKK210 pKa = 9.47 TQYY213 pKa = 11.28 TNFIDD218 pKa = 4.77 NINLSTLDD226 pKa = 3.53 TPYY229 pKa = 11.31 KK230 pKa = 10.58 LLAFYY235 pKa = 7.49 CTCRR239 pKa = 11.84 YY240 pKa = 9.72 KK241 pKa = 11.4 DD242 pKa = 3.83 NDD244 pKa = 3.6 PAANKK249 pKa = 9.59 VLYY252 pKa = 9.9 KK253 pKa = 10.32 IPSNKK258 pKa = 9.64 AVLNLIWILLTKK270 pKa = 10.16 LGYY273 pKa = 10.2 VIRR276 pKa = 11.84 STTNTKK282 pKa = 9.32 TDD284 pKa = 3.29 EE285 pKa = 4.26 TSFKK289 pKa = 10.68 VFPSFTKK296 pKa = 9.66 QSLRR300 pKa = 11.84 AKK302 pKa = 10.31 VIAALRR308 pKa = 11.84 KK309 pKa = 10.01 DD310 pKa = 3.78 GNNVEE315 pKa = 4.76 AADD318 pKa = 3.85 TLINNCEE325 pKa = 3.87 PLILFIYY332 pKa = 10.37 NFLSYY337 pKa = 10.54 PSATKK342 pKa = 10.39 ACADD346 pKa = 3.11 IAGAGKK352 pKa = 10.39 GSKK355 pKa = 10.03 EE356 pKa = 4.04 DD357 pKa = 3.56 TSGGDD362 pKa = 3.39 TLSVEE367 pKa = 4.08 NDD369 pKa = 4.42 PIPFYY374 pKa = 11.25 HH375 pKa = 7.11 NINIQEE381 pKa = 4.33 LLLTEE386 pKa = 4.47 SGEE389 pKa = 4.05 EE390 pKa = 4.04 LKK392 pKa = 10.9 NCRR395 pKa = 11.84 PFKK398 pKa = 10.68 NCIDD402 pKa = 3.76 NFKK405 pKa = 10.19 KK406 pKa = 10.39 AVYY409 pKa = 7.31 EE410 pKa = 4.08 APMKK414 pKa = 10.87 DD415 pKa = 4.48 DD416 pKa = 3.38 ILEE419 pKa = 4.24 FADD422 pKa = 4.66 SIKK425 pKa = 10.76 SSYY428 pKa = 9.76 PEE430 pKa = 3.61 HH431 pKa = 6.72 LRR433 pKa = 11.84 WNLAVSGSVDD443 pKa = 3.27 GSIINADD450 pKa = 3.83 NIVDD454 pKa = 3.58 IVEE457 pKa = 4.68 KK458 pKa = 10.68 SVQHH462 pKa = 6.47 DD463 pKa = 3.55 FNNIFRR469 pKa = 11.84 SEE471 pKa = 4.33 SEE473 pKa = 4.33 DD474 pKa = 3.28 VAAGYY479 pKa = 9.56 GIKK482 pKa = 10.37 DD483 pKa = 2.97 IFEE486 pKa = 4.15 QLEE489 pKa = 4.36 SEE491 pKa = 4.39 TTEE494 pKa = 4.32 LEE496 pKa = 3.82 SNYY499 pKa = 9.94 IVDD502 pKa = 4.23 EE503 pKa = 4.14 LAKK506 pKa = 10.37 QLVSSRR512 pKa = 11.84 QIQRR516 pKa = 11.84 MTTRR520 pKa = 11.84 PGHH523 pKa = 4.86 FLKK526 pKa = 10.8 RR527 pKa = 11.84 IPEE530 pKa = 3.81 YY531 pKa = 10.75 ALRR534 pKa = 11.84 HH535 pKa = 5.43 RR536 pKa = 11.84 SMLKK540 pKa = 8.45 TGEE543 pKa = 4.03 DD544 pKa = 2.51 VGYY547 pKa = 10.33 IMNSMVSNMKK557 pKa = 10.35 ALEE560 pKa = 4.13 KK561 pKa = 9.67 THH563 pKa = 6.09 GVEE566 pKa = 3.73 INADD570 pKa = 3.55 VKK572 pKa = 10.8 EE573 pKa = 4.55 VIAKK577 pKa = 9.38 QGNAIIKK584 pKa = 9.78 NLYY587 pKa = 9.76 SLYY590 pKa = 8.56 KK591 pKa = 8.15 TCVVVDD597 pKa = 5.01 FTRR600 pKa = 11.84 GLLEE604 pKa = 4.31 FIGRR608 pKa = 11.84 GGSANKK614 pKa = 10.01 HH615 pKa = 5.74 GLDD618 pKa = 3.36 EE619 pKa = 4.29 TDD621 pKa = 3.84 IDD623 pKa = 4.65 SEE625 pKa = 4.55 TLNVIYY631 pKa = 10.73 NHH633 pKa = 6.81 IKK635 pKa = 10.31 PSLTSNLNAYY645 pKa = 7.51 QTTINKK651 pKa = 9.63 IIEE654 pKa = 4.37 KK655 pKa = 10.37 VSGEE659 pKa = 4.17 LGVQSEE665 pKa = 4.55 EE666 pKa = 4.08 LEE668 pKa = 4.25 AANEE672 pKa = 4.04 EE673 pKa = 4.52 LFF675 pKa = 4.46
Molecular weight: 75.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.399
IPC2_protein 5.474
IPC_protein 5.461
Toseland 5.614
ProMoST 5.69
Dawson 5.575
Bjellqvist 5.652
Wikipedia 5.512
Rodwell 5.525
Grimsley 5.652
Solomon 5.575
Lehninger 5.55
Nozaki 5.766
DTASelect 5.931
Thurlkill 5.766
EMBOSS 5.728
Sillero 5.842
Patrickios 4.05
IPC_peptide 5.588
IPC2_peptide 5.83
IPC2.peptide.svr19 5.883
Protein with the highest isoelectric point:
>tr|A0A5B7QBP8|A0A5B7QBP8_9POXV Major core protein 4a precursor OS=Carp edema virus OX=1653087 GN=P4a PE=3 SV=1
MM1 pKa = 7.1 VATAAARR8 pKa = 11.84 DD9 pKa = 3.76 PEE11 pKa = 4.63 LRR13 pKa = 11.84 DD14 pKa = 3.37 SNYY17 pKa = 10.35 VMAAPAANPFANMVKK32 pKa = 10.26 FDD34 pKa = 4.47 IPDD37 pKa = 3.7 IYY39 pKa = 11.05 QFAEE43 pKa = 3.93 IGLQQIKK50 pKa = 7.88 MTTLHH55 pKa = 6.06 YY56 pKa = 10.66 CDD58 pKa = 4.84 FVSKK62 pKa = 10.46 VLPHH66 pKa = 6.55 PNYY69 pKa = 9.99 VVKK72 pKa = 10.72 KK73 pKa = 10.12 DD74 pKa = 3.12 IGYY77 pKa = 10.17 VDD79 pKa = 4.9 TIPEE83 pKa = 3.92 QVKK86 pKa = 9.9 IANAMFWKK94 pKa = 10.43 GIQIDD99 pKa = 4.22 DD100 pKa = 3.85 WNKK103 pKa = 9.57 MNVFDD108 pKa = 5.49 KK109 pKa = 11.3 TMTSNLALVLGLYY122 pKa = 10.72 DD123 pKa = 3.25 EE124 pKa = 4.61 TVYY127 pKa = 10.83 FKK129 pKa = 11.41 NSTNKK134 pKa = 9.92 FEE136 pKa = 4.3 ITFCGILDD144 pKa = 3.95 QIPQGVAVKK153 pKa = 9.74 RR154 pKa = 11.84 VCFLPYY160 pKa = 10.48 KK161 pKa = 10.2 LGNATIYY168 pKa = 8.5 KK169 pKa = 7.62 TLKK172 pKa = 10.34 HH173 pKa = 6.25 VSTQIWEE180 pKa = 4.14 GFYY183 pKa = 11.28 ANGNLITNIKK193 pKa = 9.57 QLQKK197 pKa = 9.68 VTNDD201 pKa = 3.13 EE202 pKa = 4.34 KK203 pKa = 10.87 KK204 pKa = 9.05 TLGLTKK210 pKa = 9.47 TQYY213 pKa = 11.28 TNFIDD218 pKa = 4.77 NINLSTLDD226 pKa = 3.53 TPYY229 pKa = 11.31 KK230 pKa = 10.58 LLAFYY235 pKa = 7.49 CTCRR239 pKa = 11.84 YY240 pKa = 9.72 KK241 pKa = 11.4 DD242 pKa = 3.83 NDD244 pKa = 3.6 PAANKK249 pKa = 9.59 VLYY252 pKa = 9.9 KK253 pKa = 10.32 IPSNKK258 pKa = 9.64 AVLNLIWILLTKK270 pKa = 10.16 LGYY273 pKa = 10.2 VIRR276 pKa = 11.84 STTNTKK282 pKa = 9.32 TDD284 pKa = 3.29 EE285 pKa = 4.26 TSFKK289 pKa = 10.68 VFPSFTKK296 pKa = 9.66 QSLRR300 pKa = 11.84 AKK302 pKa = 10.31 VIAALRR308 pKa = 11.84 KK309 pKa = 10.01 DD310 pKa = 3.78 GNNVEE315 pKa = 4.76 AADD318 pKa = 3.85 TLINNCEE325 pKa = 3.87 PLILFIYY332 pKa = 10.37 NFLSYY337 pKa = 10.54 PSATKK342 pKa = 10.39 ACADD346 pKa = 3.11 IAGAGKK352 pKa = 10.39 GSKK355 pKa = 10.03 EE356 pKa = 4.04 DD357 pKa = 3.56 TSGGDD362 pKa = 3.39 TLSVEE367 pKa = 4.08 NDD369 pKa = 4.42 PIPFYY374 pKa = 11.25 HH375 pKa = 7.11 NINIQEE381 pKa = 4.33 LLLTEE386 pKa = 4.47 SGEE389 pKa = 4.05 EE390 pKa = 4.04 LKK392 pKa = 10.9 NCRR395 pKa = 11.84 PFKK398 pKa = 10.68 NCIDD402 pKa = 3.76 NFKK405 pKa = 10.19 KK406 pKa = 10.39 AVYY409 pKa = 7.31 EE410 pKa = 4.08 APMKK414 pKa = 10.87 DD415 pKa = 4.48 DD416 pKa = 3.38 ILEE419 pKa = 4.24 FADD422 pKa = 4.66 SIKK425 pKa = 10.76 SSYY428 pKa = 9.76 PEE430 pKa = 3.61 HH431 pKa = 6.72 LRR433 pKa = 11.84 WNLAVSGSVDD443 pKa = 3.27 GSIINADD450 pKa = 3.83 NIVDD454 pKa = 3.58 IVEE457 pKa = 4.68 KK458 pKa = 10.68 SVQHH462 pKa = 6.47 DD463 pKa = 3.55 FNNIFRR469 pKa = 11.84 SEE471 pKa = 4.33 SEE473 pKa = 4.33 DD474 pKa = 3.28 VAAGYY479 pKa = 9.56 GIKK482 pKa = 10.37 DD483 pKa = 2.97 IFEE486 pKa = 4.15 QLEE489 pKa = 4.36 SEE491 pKa = 4.39 TTEE494 pKa = 4.32 LEE496 pKa = 3.82 SNYY499 pKa = 9.94 IVDD502 pKa = 4.23 EE503 pKa = 4.14 LAKK506 pKa = 10.37 QLVSSRR512 pKa = 11.84 QIQRR516 pKa = 11.84 MTTRR520 pKa = 11.84 PGHH523 pKa = 4.86 FLKK526 pKa = 10.8 RR527 pKa = 11.84 IPEE530 pKa = 3.81 YY531 pKa = 10.75 ALRR534 pKa = 11.84 HH535 pKa = 5.43 RR536 pKa = 11.84 SMLKK540 pKa = 8.45 TGEE543 pKa = 4.03 DD544 pKa = 2.51 VGYY547 pKa = 10.33 IMNSMVSNMKK557 pKa = 10.35 ALEE560 pKa = 4.13 KK561 pKa = 9.67 THH563 pKa = 6.09 GVEE566 pKa = 3.73 INADD570 pKa = 3.55 VKK572 pKa = 10.8 EE573 pKa = 4.55 VIAKK577 pKa = 9.38 QGNAIIKK584 pKa = 9.78 NLYY587 pKa = 9.76 SLYY590 pKa = 8.56 KK591 pKa = 8.15 TCVVVDD597 pKa = 5.01 FTRR600 pKa = 11.84 GLLEE604 pKa = 4.31 FIGRR608 pKa = 11.84 GGSANKK614 pKa = 10.01 HH615 pKa = 5.74 GLDD618 pKa = 3.36 EE619 pKa = 4.29 TDD621 pKa = 3.84 IDD623 pKa = 4.65 SEE625 pKa = 4.55 TLNVIYY631 pKa = 10.73 NHH633 pKa = 6.81 IKK635 pKa = 10.31 PSLTSNLNAYY645 pKa = 7.51 QTTINKK651 pKa = 9.63 IIEE654 pKa = 4.37 KK655 pKa = 10.37 VSGEE659 pKa = 4.17 LGVQSEE665 pKa = 4.55 EE666 pKa = 4.08 LEE668 pKa = 4.25 AANEE672 pKa = 4.04 EE673 pKa = 4.52 LFF675 pKa = 4.46
Molecular weight: 75.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.399
IPC2_protein 5.474
IPC_protein 5.461
Toseland 5.614
ProMoST 5.69
Dawson 5.575
Bjellqvist 5.652
Wikipedia 5.512
Rodwell 5.525
Grimsley 5.652
Solomon 5.575
Lehninger 5.55
Nozaki 5.766
DTASelect 5.931
Thurlkill 5.766
EMBOSS 5.728
Sillero 5.842
Patrickios 4.05
IPC_peptide 5.588
IPC2_peptide 5.83
IPC2.peptide.svr19 5.883
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
675
675
675
675.0
75.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.963 ± 0.0
1.481 ± 0.0
6.074 ± 0.0
6.37 ± 0.0
4.296 ± 0.0
5.037 ± 0.0
1.63 ± 0.0
8.148 ± 0.0
8.148 ± 0.0
8.741 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.926 ± 0.0
7.704 ± 0.0
3.407 ± 0.0
3.111 ± 0.0
2.667 ± 0.0
5.926 ± 0.0
6.815 ± 0.0
6.519 ± 0.0
0.741 ± 0.0
4.296 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here