Capybara microvirus Cap3_SP_586
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8X8|A0A4P8W8X8_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_586 OX=2585479 PE=4 SV=1
MM1 pKa = 7.32 FRR3 pKa = 11.84 NFCDD7 pKa = 3.3 IILRR11 pKa = 11.84 IMPSVSPQVFSSTSITQSGMDD32 pKa = 3.22 VSLRR36 pKa = 11.84 DD37 pKa = 3.83 MISKK41 pKa = 9.8 YY42 pKa = 7.26 VTYY45 pKa = 10.14 GVPIPDD51 pKa = 3.33 VGGEE55 pKa = 4.2 YY56 pKa = 10.81 YY57 pKa = 11.03 GDD59 pKa = 3.91 DD60 pKa = 3.91 TPDD63 pKa = 3.26 VNPFGFDD70 pKa = 3.64 LEE72 pKa = 4.88 DD73 pKa = 3.7 SPKK76 pKa = 10.06 VRR78 pKa = 11.84 QDD80 pKa = 3.45 YY81 pKa = 11.16 INAVNNAQALQNEE94 pKa = 4.56 QTSQNASAMSPQNAQLAEE112 pKa = 4.17 KK113 pKa = 10.67 DD114 pKa = 3.73 RR115 pKa = 11.84 VNVASEE121 pKa = 4.08 RR122 pKa = 11.84 ASEE125 pKa = 3.99 ASEE128 pKa = 3.82 RR129 pKa = 11.84 ASDD132 pKa = 3.16 ITAPLEE138 pKa = 3.93
Molecular weight: 15.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.297
IPC2_protein 4.151
IPC_protein 4.088
Toseland 3.884
ProMoST 4.19
Dawson 4.075
Bjellqvist 4.279
Wikipedia 3.999
Rodwell 3.923
Grimsley 3.795
Solomon 4.062
Lehninger 4.012
Nozaki 4.19
DTASelect 4.406
Thurlkill 3.935
EMBOSS 3.999
Sillero 4.202
Patrickios 3.694
IPC_peptide 4.062
IPC2_peptide 4.19
IPC2.peptide.svr19 4.14
Protein with the highest isoelectric point:
>tr|A0A4P8W555|A0A4P8W555_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_586 OX=2585479 PE=3 SV=1
MM1 pKa = 7.11 MCQSPIHH8 pKa = 6.42 LRR10 pKa = 11.84 DD11 pKa = 3.38 GPLRR15 pKa = 11.84 FVPCGKK21 pKa = 9.87 CANCLKK27 pKa = 10.27 RR28 pKa = 11.84 KK29 pKa = 8.81 HH30 pKa = 6.57 LEE32 pKa = 3.6 WQFRR36 pKa = 11.84 LQRR39 pKa = 11.84 EE40 pKa = 4.27 EE41 pKa = 4.78 RR42 pKa = 11.84 SSKK45 pKa = 10.43 CCYY48 pKa = 10.18 CVTLTYY54 pKa = 10.77 NEE56 pKa = 4.54 SNVPISWTSKK66 pKa = 10.34 NEE68 pKa = 3.69 GGNLIFTLCKK78 pKa = 10.14 SDD80 pKa = 3.27 VQAWLKK86 pKa = 10.48 RR87 pKa = 11.84 IRR89 pKa = 11.84 HH90 pKa = 5.3 YY91 pKa = 10.85 LPKK94 pKa = 10.04 FRR96 pKa = 11.84 YY97 pKa = 8.97 YY98 pKa = 10.98 LVGEE102 pKa = 4.48 YY103 pKa = 8.36 GTKK106 pKa = 8.32 TLRR109 pKa = 11.84 PHH111 pKa = 4.87 YY112 pKa = 10.07 HH113 pKa = 6.13 VILFFSDD120 pKa = 3.63 YY121 pKa = 11.17 CNLQSVFDD129 pKa = 3.55 ITFKK133 pKa = 10.71 YY134 pKa = 8.17 WCKK137 pKa = 10.87 SDD139 pKa = 3.93 IEE141 pKa = 4.25 GHH143 pKa = 5.95 KK144 pKa = 10.49 VDD146 pKa = 4.19 IVSPQAINYY155 pKa = 7.39 CSKK158 pKa = 10.62 YY159 pKa = 8.17 VQKK162 pKa = 10.98 LMSLPSWLDD171 pKa = 3.32 CQPPFALMSRR181 pKa = 11.84 KK182 pKa = 9.47 PGIGDD187 pKa = 3.38 SFIRR191 pKa = 11.84 NEE193 pKa = 3.78 ATATEE198 pKa = 4.11 ILSNYY203 pKa = 8.58 RR204 pKa = 11.84 SQVFFDD210 pKa = 3.18 EE211 pKa = 4.48 GHH213 pKa = 6.38 RR214 pKa = 11.84 VTLPRR219 pKa = 11.84 FYY221 pKa = 10.39 RR222 pKa = 11.84 DD223 pKa = 3.56 KK224 pKa = 11.56 LFIDD228 pKa = 4.07 NLDD231 pKa = 3.39 KK232 pKa = 11.03 QIYY235 pKa = 8.91 RR236 pKa = 11.84 DD237 pKa = 3.09 KK238 pKa = 10.82 CKK240 pKa = 10.37 RR241 pKa = 11.84 YY242 pKa = 9.46 FDD244 pKa = 4.61 NYY246 pKa = 8.94 TNQNFANYY254 pKa = 8.17 VNKK257 pKa = 10.3 FGFHH261 pKa = 4.88 QACKK265 pKa = 10.02 FFSDD269 pKa = 3.76 MPKK272 pKa = 10.56 NDD274 pKa = 3.57 LQKK277 pKa = 10.96 LIRR280 pKa = 11.84 FFKK283 pKa = 10.76 NKK285 pKa = 9.39 QKK287 pKa = 11.05 LL288 pKa = 3.49
Molecular weight: 34.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.777
IPC2_protein 8.668
IPC_protein 8.536
Toseland 9.019
ProMoST 9.033
Dawson 9.428
Bjellqvist 9.37
Wikipedia 9.575
Rodwell 9.589
Grimsley 9.428
Solomon 9.472
Lehninger 9.428
Nozaki 9.516
DTASelect 9.224
Thurlkill 9.326
EMBOSS 9.56
Sillero 9.516
Patrickios 4.291
IPC_peptide 9.458
IPC2_peptide 8.317
IPC2.peptide.svr19 7.797
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1364
79
553
272.8
31.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.158 ± 1.379
1.613 ± 0.997
5.792 ± 0.554
4.619 ± 0.399
6.158 ± 0.975
5.425 ± 0.987
1.906 ± 0.336
5.865 ± 0.322
6.158 ± 0.938
9.238 ± 0.788
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.493 ± 0.515
6.965 ± 1.087
4.692 ± 0.513
5.059 ± 0.433
4.545 ± 0.629
7.625 ± 0.825
5.059 ± 0.647
4.692 ± 0.509
1.246 ± 0.209
4.692 ± 0.426
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here