Liao ning virus
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2TV14|Q2TV14_9REOV VP6 OS=Liao ning virus OX=246280 PE=4 SV=1
MM1 pKa = 8.25 DD2 pKa = 4.91 IPSPNCGSVQKK13 pKa = 11.03 NCFQVYY19 pKa = 9.93 KK20 pKa = 9.59 SQNVYY25 pKa = 9.86 RR26 pKa = 11.84 VRR28 pKa = 11.84 FCVEE32 pKa = 3.85 GKK34 pKa = 9.48 IHH36 pKa = 7.16 DD37 pKa = 4.05 VPLPGVNTHH46 pKa = 6.49 RR47 pKa = 11.84 DD48 pKa = 3.05 IIGTVKK54 pKa = 10.1 YY55 pKa = 9.98 FCEE58 pKa = 4.0 LMGLNIVDD66 pKa = 3.48 NSLRR70 pKa = 11.84 LNWLNLSDD78 pKa = 4.07 MKK80 pKa = 11.42 NHH82 pKa = 6.66 GDD84 pKa = 3.43 NVTLAKK90 pKa = 10.5 VGSEE94 pKa = 4.0 YY95 pKa = 11.47 GDD97 pKa = 3.92 LFVKK101 pKa = 10.47 KK102 pKa = 10.56 LPTLPINIHH111 pKa = 4.28 STNYY115 pKa = 9.6 VFGKK119 pKa = 9.2 YY120 pKa = 8.59 SVFARR125 pKa = 11.84 VHH127 pKa = 6.37 GIVKK131 pKa = 10.36 LLNDD135 pKa = 3.92 DD136 pKa = 4.18 NYY138 pKa = 11.49 DD139 pKa = 3.38 VGVILEE145 pKa = 4.25 KK146 pKa = 10.95 CNEE149 pKa = 3.81 IRR151 pKa = 11.84 VMTVNIIVMGLKK163 pKa = 10.34 SLMDD167 pKa = 3.23 SHH169 pKa = 7.48 RR170 pKa = 11.84 EE171 pKa = 3.99 SGGTLHH177 pKa = 7.54 GDD179 pKa = 3.36 CTVQNLMCDD188 pKa = 3.08 KK189 pKa = 10.92 FGMLKK194 pKa = 10.55 LVDD197 pKa = 4.57 PGNLMSQTVIWKK209 pKa = 9.96 NDD211 pKa = 2.98 TQYY214 pKa = 11.37 FGNTVDD220 pKa = 6.01 DD221 pKa = 4.59 EE222 pKa = 4.77 VCSFVMACLKK232 pKa = 8.0 TTANLRR238 pKa = 11.84 NIEE241 pKa = 4.15 TSEE244 pKa = 3.8 LTIPFITDD252 pKa = 4.18 LYY254 pKa = 11.44 LLDD257 pKa = 4.48 TVLSDD262 pKa = 3.75 PNVIHH267 pKa = 6.44 GTRR270 pKa = 11.84 EE271 pKa = 4.22 SGWLEE276 pKa = 3.94 VGSDD280 pKa = 3.8 FVSIVQMFVALANMVRR296 pKa = 11.84 PYY298 pKa = 11.27 NLDD301 pKa = 3.6 DD302 pKa = 3.72 VVSALKK308 pKa = 9.02 TQVTGEE314 pKa = 4.26 NEE316 pKa = 4.01 TFSNVIEE323 pKa = 4.0 KK324 pKa = 10.65 HH325 pKa = 5.86 EE326 pKa = 5.01 DD327 pKa = 3.6 GEE329 pKa = 4.81 HH330 pKa = 6.87 PDD332 pKa = 3.59 SDD334 pKa = 4.72 LEE336 pKa = 4.16
Molecular weight: 37.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.854
IPC2_protein 4.927
IPC_protein 4.889
Toseland 4.825
ProMoST 4.991
Dawson 4.902
Bjellqvist 5.054
Wikipedia 4.813
Rodwell 4.8
Grimsley 4.749
Solomon 4.902
Lehninger 4.863
Nozaki 5.029
DTASelect 5.232
Thurlkill 4.838
EMBOSS 4.851
Sillero 5.092
Patrickios 3.668
IPC_peptide 4.914
IPC2_peptide 5.08
IPC2.peptide.svr19 5.026
Protein with the highest isoelectric point:
>tr|Q2TV15|Q2TV15_9REOV VP5 OS=Liao ning virus OX=246280 PE=4 SV=1
MM1 pKa = 7.58 SKK3 pKa = 10.79 LSTIPRR9 pKa = 11.84 TRR11 pKa = 11.84 SQVVGLTSKK20 pKa = 11.01 LKK22 pKa = 10.88 DD23 pKa = 3.37 FTKK26 pKa = 10.56 SVEE29 pKa = 4.1 KK30 pKa = 8.54 TTLLTQSEE38 pKa = 4.68 LKK40 pKa = 9.99 KK41 pKa = 9.38 QHH43 pKa = 5.96 RR44 pKa = 11.84 HH45 pKa = 5.67 LLSQQPEE52 pKa = 4.58 LFSGLAKK59 pKa = 10.68 LYY61 pKa = 9.31 TSNVSKK67 pKa = 10.63 FEE69 pKa = 4.39 RR70 pKa = 11.84 IIRR73 pKa = 11.84 LYY75 pKa = 10.38 GGQGSKK81 pKa = 10.72 HH82 pKa = 6.52 SISSVGFSHH91 pKa = 8.01 LIEE94 pKa = 4.25 YY95 pKa = 9.52 CNNYY99 pKa = 9.93 DD100 pKa = 5.58 LPDD103 pKa = 4.88 DD104 pKa = 5.2 DD105 pKa = 4.75 STLKK109 pKa = 10.75 AICFTIFQIHH119 pKa = 6.06 SNEE122 pKa = 3.75 LRR124 pKa = 11.84 NGGANALFTIASEE137 pKa = 4.43 YY138 pKa = 10.75 FNAPAVKK145 pKa = 9.78 EE146 pKa = 4.01 IVGEE150 pKa = 3.98 HH151 pKa = 5.25 VKK153 pKa = 10.39 IKK155 pKa = 10.36 SCNKK159 pKa = 8.91 VPVAIVNFKK168 pKa = 10.83 ALTQIIPYY176 pKa = 9.67 RR177 pKa = 11.84 GVKK180 pKa = 9.79 ALRR183 pKa = 11.84 TKK185 pKa = 10.79 LLEE188 pKa = 4.46 TQDD191 pKa = 3.84 GVALEE196 pKa = 5.09 HH197 pKa = 6.87 IGSFYY202 pKa = 9.92 NTCKK206 pKa = 10.5 INGEE210 pKa = 3.72 FDD212 pKa = 3.45 AVSFCGLKK220 pKa = 10.31 LRR222 pKa = 11.84 QSCIVSVAGHH232 pKa = 7.26 AIDD235 pKa = 5.03 VGPDD239 pKa = 3.08 YY240 pKa = 10.54 YY241 pKa = 11.0 RR242 pKa = 11.84 QMLAEE247 pKa = 4.18 VTALLKK253 pKa = 10.64 LKK255 pKa = 10.28 KK256 pKa = 9.96 KK257 pKa = 9.08 VGSVVMHH264 pKa = 6.74 GNNQFGFVKK273 pKa = 10.29 PLDD276 pKa = 3.84 ASQTQNVTQIRR287 pKa = 11.84 KK288 pKa = 8.99 ILNDD292 pKa = 3.32 ASRR295 pKa = 11.84 VSFINVRR302 pKa = 11.84 NAASRR307 pKa = 11.84 DD308 pKa = 3.65 EE309 pKa = 4.21 MVYY312 pKa = 10.46 AYY314 pKa = 9.6 DD315 pKa = 3.93 PCLLYY320 pKa = 11.12 YY321 pKa = 9.4 ITIPNNGNKK330 pKa = 8.65 SHH332 pKa = 7.52 CIQALNLSGLLEE344 pKa = 4.05 HH345 pKa = 7.19 RR346 pKa = 11.84 LLHH349 pKa = 6.25 FGSLGTSCRR358 pKa = 11.84 DD359 pKa = 3.15 PSCRR363 pKa = 11.84 VIGIDD368 pKa = 3.66 RR369 pKa = 11.84 PEE371 pKa = 4.75 LYY373 pKa = 10.44 SEE375 pKa = 4.69 LNRR378 pKa = 11.84 IVDD381 pKa = 3.37 NTTIKK386 pKa = 10.82 DD387 pKa = 3.33 RR388 pKa = 11.84 ADD390 pKa = 3.14 AMLEE394 pKa = 3.87 INADD398 pKa = 3.4 EE399 pKa = 4.11 MSSVKK404 pKa = 10.6 SSGSGKK410 pKa = 10.43 SLTKK414 pKa = 10.33 GKK416 pKa = 9.48 YY417 pKa = 8.57 VRR419 pKa = 11.84 NSIDD423 pKa = 3.06 KK424 pKa = 10.4 GHH426 pKa = 6.7 NLTDD430 pKa = 3.63 LGKK433 pKa = 10.71 ANGDD437 pKa = 3.67 QYY439 pKa = 11.77 SHH441 pKa = 6.82 YY442 pKa = 10.71 SQMSKK447 pKa = 10.64 PSGSEE452 pKa = 3.0 ISKK455 pKa = 10.64 FITAEE460 pKa = 3.88 AVKK463 pKa = 10.44 GISDD467 pKa = 3.78 SSLDD471 pKa = 3.61 ATEE474 pKa = 5.21 LSLKK478 pKa = 10.57 LAMLQSSAVTIGASKK493 pKa = 11.29 NEE495 pKa = 4.14 TKK497 pKa = 10.39 PVEE500 pKa = 4.38 TKK502 pKa = 10.04 VVKK505 pKa = 10.55 APSVNKK511 pKa = 9.05 TADD514 pKa = 3.52 EE515 pKa = 4.29 LEE517 pKa = 4.58 EE518 pKa = 4.05 II519 pKa = 4.67
Molecular weight: 57.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.261
IPC2_protein 8.244
IPC_protein 8.083
Toseland 8.551
ProMoST 8.668
Dawson 9.019
Bjellqvist 9.033
Wikipedia 9.092
Rodwell 9.194
Grimsley 8.77
Solomon 9.092
Lehninger 9.077
Nozaki 9.209
DTASelect 8.873
Thurlkill 8.99
EMBOSS 9.194
Sillero 9.209
Patrickios 4.609
IPC_peptide 9.092
IPC2_peptide 7.732
IPC2.peptide.svr19 7.704
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
6079
174
1222
506.6
56.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.284 ± 0.511
1.25 ± 0.241
6.317 ± 0.409
4.951 ± 0.196
3.866 ± 0.361
5.79 ± 0.272
2.5 ± 0.348
6.498 ± 0.383
5.083 ± 0.456
9.048 ± 0.279
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.158 ± 0.218
6.662 ± 0.296
3.915 ± 0.14
2.632 ± 0.186
5.593 ± 0.352
8.11 ± 0.446
6.021 ± 0.195
7.715 ± 0.339
0.592 ± 0.091
4.014 ± 0.368
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here