Amino acid dipepetide frequency for Rhizobiales bacterium GAS113

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.435AlaAla: 18.435 ± 0.115	1.245AlaCys: 1.245 ± 0.022	6.388AlaAsp: 6.388 ± 0.049	7.502AlaGlu: 7.502 ± 0.065	4.627AlaPhe: 4.627 ± 0.045	11.148AlaGly: 11.148 ± 0.09	2.514AlaHis: 2.514 ± 0.031	6.746AlaIle: 6.746 ± 0.057	4.454AlaLys: 4.454 ± 0.052	14.194AlaLeu: 14.194 ± 0.104	3.477AlaMet: 3.477 ± 0.035	2.949AlaAsn: 2.949 ± 0.045	6.073AlaPro: 6.073 ± 0.057	4.161AlaGln: 4.161 ± 0.044	9.522AlaArg: 9.522 ± 0.072	7.452AlaSer: 7.452 ± 0.059	6.422AlaThr: 6.422 ± 0.054	8.952AlaVal: 8.952 ± 0.059	1.604AlaTrp: 1.604 ± 0.026	2.727AlaTyr: 2.727 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	1.125CysAla: 1.125 ± 0.023	0.134CysCys: 0.134 ± 0.009	0.535CysAsp: 0.535 ± 0.013	0.492CysGlu: 0.492 ± 0.014	0.358CysPhe: 0.358 ± 0.014	1.025CysGly: 1.025 ± 0.021	0.256CysHis: 0.256 ± 0.01	0.386CysIle: 0.386 ± 0.011	0.198CysLys: 0.198 ± 0.01	0.904CysLeu: 0.904 ± 0.021	0.163CysMet: 0.163 ± 0.008	0.214CysAsn: 0.214 ± 0.008	0.481CysPro: 0.481 ± 0.013	0.211CysGln: 0.211 ± 0.009	0.739CysArg: 0.739 ± 0.018	0.459CysSer: 0.459 ± 0.012	0.361CysThr: 0.361 ± 0.011	0.708CysVal: 0.708 ± 0.015	0.117CysTrp: 0.117 ± 0.007	0.208CysTyr: 0.208 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	6.679AspAla: 6.679 ± 0.056	0.489AspCys: 0.489 ± 0.014	2.6AspAsp: 2.6 ± 0.032	3.193AspGlu: 3.193 ± 0.041	1.964AspPhe: 1.964 ± 0.028	4.688AspGly: 4.688 ± 0.048	1.104AspHis: 1.104 ± 0.021	2.76AspIle: 2.76 ± 0.035	1.638AspLys: 1.638 ± 0.029	5.735AspLeu: 5.735 ± 0.06	1.159AspMet: 1.159 ± 0.022	1.131AspAsn: 1.131 ± 0.023	3.547AspPro: 3.547 ± 0.036	1.651AspGln: 1.651 ± 0.027	4.063AspArg: 4.063 ± 0.041	1.938AspSer: 1.938 ± 0.027	2.338AspThr: 2.338 ± 0.032	3.665AspVal: 3.665 ± 0.04	0.902AspTrp: 0.902 ± 0.017	1.353AspTyr: 1.353 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	7.924GluAla: 7.924 ± 0.063	0.348GluCys: 0.348 ± 0.011	2.377GluAsp: 2.377 ± 0.028	2.794GluGlu: 2.794 ± 0.037	1.774GluPhe: 1.774 ± 0.025	3.975GluGly: 3.975 ± 0.042	1.262GluHis: 1.262 ± 0.025	3.321GluIle: 3.321 ± 0.03	1.837GluLys: 1.837 ± 0.029	5.59GluLeu: 5.59 ± 0.058	1.389GluMet: 1.389 ± 0.026	1.208GluAsn: 1.208 ± 0.021	2.866GluPro: 2.866 ± 0.037	1.757GluGln: 1.757 ± 0.023	5.277GluArg: 5.277 ± 0.061	2.235GluSer: 2.235 ± 0.03	3.127GluThr: 3.127 ± 0.034	3.601GluVal: 3.601 ± 0.043	0.675GluTrp: 0.675 ± 0.018	0.852GluTyr: 0.852 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	5.02PheAla: 5.02 ± 0.044	0.388PheCys: 0.388 ± 0.012	2.535PheAsp: 2.535 ± 0.034	2.137PheGlu: 2.137 ± 0.028	1.358PhePhe: 1.358 ± 0.024	3.777PheGly: 3.777 ± 0.044	0.747PheHis: 0.747 ± 0.017	1.641PheIle: 1.641 ± 0.024	0.976PheLys: 0.976 ± 0.021	3.453PheLeu: 3.453 ± 0.04	0.718PheMet: 0.718 ± 0.015	1.0PheAsn: 1.0 ± 0.022	1.681PhePro: 1.681 ± 0.027	1.003PheGln: 1.003 ± 0.021	2.353PheArg: 2.353 ± 0.031	2.07PheSer: 2.07 ± 0.031	1.905PheThr: 1.905 ± 0.029	2.935PheVal: 2.935 ± 0.039	0.554PheTrp: 0.554 ± 0.015	0.845PheTyr: 0.845 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	10.063GlyAla: 10.063 ± 0.08	0.88GlyCys: 0.88 ± 0.017	4.168GlyAsp: 4.168 ± 0.046	4.817GlyGlu: 4.817 ± 0.042	3.671GlyPhe: 3.671 ± 0.039	7.414GlyGly: 7.414 ± 0.105	1.851GlyHis: 1.851 ± 0.028	4.453GlyIle: 4.453 ± 0.048	3.164GlyLys: 3.164 ± 0.04	9.403GlyLeu: 9.403 ± 0.067	2.137GlyMet: 2.137 ± 0.029	2.027GlyAsn: 2.027 ± 0.036	3.684GlyPro: 3.684 ± 0.042	2.78GlyGln: 2.78 ± 0.034	6.583GlyArg: 6.583 ± 0.052	4.988GlySer: 4.988 ± 0.064	4.39GlyThr: 4.39 ± 0.077	6.317GlyVal: 6.317 ± 0.056	1.338GlyTrp: 1.338 ± 0.025	2.234GlyTyr: 2.234 ± 0.032	0.0GlyXaa: 0.0 ± 0.0
His	2.425HisAla: 2.425 ± 0.034	0.26HisCys: 0.26 ± 0.011	1.207HisAsp: 1.207 ± 0.022	1.097HisGlu: 1.097 ± 0.02	0.794HisPhe: 0.794 ± 0.017	1.992HisGly: 1.992 ± 0.029	0.55HisHis: 0.55 ± 0.02	0.955HisIle: 0.955 ± 0.016	0.497HisLys: 0.497 ± 0.014	2.235HisLeu: 2.235 ± 0.034	0.472HisMet: 0.472 ± 0.014	0.456HisAsn: 0.456 ± 0.012	1.379HisPro: 1.379 ± 0.027	0.596HisGln: 0.596 ± 0.015	1.527HisArg: 1.527 ± 0.022	0.995HisSer: 0.995 ± 0.019	0.738HisThr: 0.738 ± 0.017	1.552HisVal: 1.552 ± 0.023	0.348HisTrp: 0.348 ± 0.011	0.526HisTyr: 0.526 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	7.774IleAla: 7.774 ± 0.057	0.567IleCys: 0.567 ± 0.014	3.393IleAsp: 3.393 ± 0.032	3.457IleGlu: 3.457 ± 0.037	1.712IlePhe: 1.712 ± 0.028	5.362IleGly: 5.362 ± 0.053	0.861IleHis: 0.861 ± 0.019	2.186IleIle: 2.186 ± 0.031	1.406IleLys: 1.406 ± 0.025	4.528IleLeu: 4.528 ± 0.049	0.898IleMet: 0.898 ± 0.02	1.233IleAsn: 1.233 ± 0.027	2.246IlePro: 2.246 ± 0.031	1.099IleGln: 1.099 ± 0.021	3.18IleArg: 3.18 ± 0.036	2.811IleSer: 2.811 ± 0.038	2.476IleThr: 2.476 ± 0.033	4.317IleVal: 4.317 ± 0.04	0.633IleTrp: 0.633 ± 0.017	1.102IleTyr: 1.102 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	4.272LysAla: 4.272 ± 0.051	0.167LysCys: 0.167 ± 0.008	1.582LysAsp: 1.582 ± 0.032	1.51LysGlu: 1.51 ± 0.026	0.863LysPhe: 0.863 ± 0.019	2.623LysGly: 2.623 ± 0.033	0.56LysHis: 0.56 ± 0.015	1.644LysIle: 1.644 ± 0.023	0.988LysLys: 0.988 ± 0.023	3.494LysLeu: 3.494 ± 0.037	0.647LysMet: 0.647 ± 0.017	0.714LysAsn: 0.714 ± 0.016	2.101LysPro: 2.101 ± 0.029	0.936LysGln: 0.936 ± 0.019	2.443LysArg: 2.443 ± 0.04	1.799LysSer: 1.799 ± 0.027	1.861LysThr: 1.861 ± 0.031	2.291LysVal: 2.291 ± 0.035	0.351LysTrp: 0.351 ± 0.013	0.506LysTyr: 0.506 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	15.274LeuAla: 15.274 ± 0.115	1.016LeuCys: 1.016 ± 0.02	5.976LeuAsp: 5.976 ± 0.048	5.105LeuGlu: 5.105 ± 0.06	3.621LeuPhe: 3.621 ± 0.044	8.933LeuGly: 8.933 ± 0.075	1.899LeuHis: 1.899 ± 0.028	5.231LeuIle: 5.231 ± 0.052	3.329LeuLys: 3.329 ± 0.036	9.701LeuLeu: 9.701 ± 0.094	2.247LeuMet: 2.247 ± 0.029	2.384LeuAsn: 2.384 ± 0.037	5.756LeuPro: 5.756 ± 0.057	2.837LeuGln: 2.837 ± 0.038	7.805LeuArg: 7.805 ± 0.068	6.572LeuSer: 6.572 ± 0.061	5.624LeuThr: 5.624 ± 0.062	7.877LeuVal: 7.877 ± 0.057	1.186LeuTrp: 1.186 ± 0.021	2.017LeuTyr: 2.017 ± 0.029	0.0LeuXaa: 0.0 ± 0.0
Met	2.898MetAla: 2.898 ± 0.033	0.152MetCys: 0.152 ± 0.007	0.894MetAsp: 0.894 ± 0.017	0.888MetGlu: 0.888 ± 0.02	0.638MetPhe: 0.638 ± 0.015	1.626MetGly: 1.626 ± 0.026	0.438MetHis: 0.438 ± 0.013	1.235MetIle: 1.235 ± 0.021	0.796MetLys: 0.796 ± 0.018	2.467MetLeu: 2.467 ± 0.028	0.583MetMet: 0.583 ± 0.016	0.679MetAsn: 0.679 ± 0.016	1.433MetPro: 1.433 ± 0.021	0.815MetGln: 0.815 ± 0.02	2.064MetArg: 2.064 ± 0.029	1.672MetSer: 1.672 ± 0.026	1.764MetThr: 1.764 ± 0.024	1.512MetVal: 1.512 ± 0.026	0.195MetTrp: 0.195 ± 0.008	0.306MetTyr: 0.306 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	3.071AsnAla: 3.071 ± 0.049	0.229AsnCys: 0.229 ± 0.009	1.171AsnAsp: 1.171 ± 0.023	1.067AsnGlu: 1.067 ± 0.02	0.913AsnPhe: 0.913 ± 0.019	2.301AsnGly: 2.301 ± 0.047	0.476AsnHis: 0.476 ± 0.012	1.23AsnIle: 1.23 ± 0.025	0.608AsnLys: 0.608 ± 0.016	2.552AsnLeu: 2.552 ± 0.032	0.518AsnMet: 0.518 ± 0.014	0.634AsnAsn: 0.634 ± 0.023	1.738AsnPro: 1.738 ± 0.031	0.686AsnGln: 0.686 ± 0.016	1.66AsnArg: 1.66 ± 0.025	1.284AsnSer: 1.284 ± 0.031	1.171AsnThr: 1.171 ± 0.03	1.719AsnVal: 1.719 ± 0.028	0.401AsnTrp: 0.401 ± 0.014	0.627AsnTyr: 0.627 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	6.657ProAla: 6.657 ± 0.063	0.389ProCys: 0.389 ± 0.011	3.339ProAsp: 3.339 ± 0.04	3.276ProGlu: 3.276 ± 0.034	2.015ProPhe: 2.015 ± 0.027	4.705ProGly: 4.705 ± 0.044	1.183ProHis: 1.183 ± 0.022	2.557ProIle: 2.557 ± 0.032	1.777ProLys: 1.777 ± 0.024	5.056ProLeu: 5.056 ± 0.047	1.202ProMet: 1.202 ± 0.021	1.396ProAsn: 1.396 ± 0.025	3.0ProPro: 3.0 ± 0.049	1.852ProGln: 1.852 ± 0.031	3.413ProArg: 3.413 ± 0.043	3.388ProSer: 3.388 ± 0.037	2.601ProThr: 2.601 ± 0.034	3.979ProVal: 3.979 ± 0.044	0.72ProTrp: 0.72 ± 0.014	1.241ProTyr: 1.241 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	4.34GlnAla: 4.34 ± 0.043	0.212GlnCys: 0.212 ± 0.01	1.496GlnAsp: 1.496 ± 0.026	1.448GlnGlu: 1.448 ± 0.025	1.038GlnPhe: 1.038 ± 0.019	2.578GlnGly: 2.578 ± 0.029	0.625GlnHis: 0.625 ± 0.016	1.74GlnIle: 1.74 ± 0.033	0.96GlnLys: 0.96 ± 0.019	2.968GlnLeu: 2.968 ± 0.039	0.773GlnMet: 0.773 ± 0.018	0.799GlnAsn: 0.799 ± 0.018	1.738GlnPro: 1.738 ± 0.03	1.1GlnGln: 1.1 ± 0.024	2.547GlnArg: 2.547 ± 0.033	1.737GlnSer: 1.737 ± 0.029	1.554GlnThr: 1.554 ± 0.031	2.084GlnVal: 2.084 ± 0.031	0.398GlnTrp: 0.398 ± 0.013	0.576GlnTyr: 0.576 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	8.556ArgAla: 8.556 ± 0.068	0.662ArgCys: 0.662 ± 0.015	4.0ArgAsp: 4.0 ± 0.039	4.266ArgGlu: 4.266 ± 0.044	3.1ArgPhe: 3.1 ± 0.037	5.073ArgGly: 5.073 ± 0.047	1.926ArgHis: 1.926 ± 0.026	4.089ArgIle: 4.089 ± 0.044	2.371ArgLys: 2.371 ± 0.033	8.917ArgLeu: 8.917 ± 0.077	1.825ArgMet: 1.825 ± 0.024	1.828ArgAsn: 1.828 ± 0.031	3.954ArgPro: 3.954 ± 0.046	2.729ArgGln: 2.729 ± 0.037	6.875ArgArg: 6.875 ± 0.065	4.319ArgSer: 4.319 ± 0.04	3.195ArgThr: 3.195 ± 0.037	4.821ArgVal: 4.821 ± 0.046	1.032ArgTrp: 1.032 ± 0.021	1.773ArgTyr: 1.773 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	6.613SerAla: 6.613 ± 0.06	0.521SerCys: 0.521 ± 0.014	2.704SerAsp: 2.704 ± 0.03	2.696SerGlu: 2.696 ± 0.034	2.614SerPhe: 2.614 ± 0.036	5.711SerGly: 5.711 ± 0.067	1.121SerHis: 1.121 ± 0.019	2.865SerIle: 2.865 ± 0.032	1.572SerLys: 1.572 ± 0.025	6.015SerLeu: 6.015 ± 0.058	1.271SerMet: 1.271 ± 0.019	1.312SerAsn: 1.312 ± 0.025	3.199SerPro: 3.199 ± 0.034	1.656SerGln: 1.656 ± 0.026	3.929SerArg: 3.929 ± 0.034	3.258SerSer: 3.258 ± 0.051	2.828SerThr: 2.828 ± 0.051	4.098SerVal: 4.098 ± 0.045	0.882SerTrp: 0.882 ± 0.019	1.35SerTyr: 1.35 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	6.103ThrAla: 6.103 ± 0.062	0.397ThrCys: 0.397 ± 0.011	2.45ThrAsp: 2.45 ± 0.037	2.357ThrGlu: 2.357 ± 0.031	1.861ThrPhe: 1.861 ± 0.029	4.81ThrGly: 4.81 ± 0.054	1.04ThrHis: 1.04 ± 0.021	2.913ThrIle: 2.913 ± 0.033	1.519ThrLys: 1.519 ± 0.022	5.913ThrLeu: 5.913 ± 0.066	1.069ThrMet: 1.069 ± 0.019	1.339ThrAsn: 1.339 ± 0.038	3.261ThrPro: 3.261 ± 0.037	1.604ThrGln: 1.604 ± 0.043	3.351ThrArg: 3.351 ± 0.037	2.969ThrSer: 2.969 ± 0.049	3.044ThrThr: 3.044 ± 0.084	3.96ThrVal: 3.96 ± 0.058	0.597ThrTrp: 0.597 ± 0.015	1.156ThrTyr: 1.156 ± 0.021	0.0ThrXaa: 0.0 ± 0.0
Val	9.587ValAla: 9.587 ± 0.065	0.62ValCys: 0.62 ± 0.015	3.878ValAsp: 3.878 ± 0.039	4.363ValGlu: 4.363 ± 0.037	2.754ValPhe: 2.754 ± 0.035	5.603ValGly: 5.603 ± 0.058	1.387ValHis: 1.387 ± 0.026	3.893ValIle: 3.893 ± 0.038	2.285ValLys: 2.285 ± 0.032	7.334ValLeu: 7.334 ± 0.063	1.772ValMet: 1.772 ± 0.024	1.807ValAsn: 1.807 ± 0.032	3.776ValPro: 3.776 ± 0.041	2.008ValGln: 2.008 ± 0.028	4.8ValArg: 4.8 ± 0.047	4.285ValSer: 4.285 ± 0.043	4.491ValThr: 4.491 ± 0.061	5.867ValVal: 5.867 ± 0.048	0.817ValTrp: 0.817 ± 0.017	1.37ValTyr: 1.37 ± 0.023	0.0ValXaa: 0.0 ± 0.0
Trp	1.185TrpAla: 1.185 ± 0.022	0.153TrpCys: 0.153 ± 0.006	0.58TrpAsp: 0.58 ± 0.017	0.547TrpGlu: 0.547 ± 0.013	0.531TrpPhe: 0.531 ± 0.013	0.937TrpGly: 0.937 ± 0.019	0.34TrpHis: 0.34 ± 0.011	0.635TrpIle: 0.635 ± 0.017	0.43TrpLys: 0.43 ± 0.011	1.608TrpLeu: 1.608 ± 0.026	0.347TrpMet: 0.347 ± 0.01	0.43TrpAsn: 0.43 ± 0.013	0.752TrpPro: 0.752 ± 0.016	0.535TrpGln: 0.535 ± 0.012	1.384TrpArg: 1.384 ± 0.025	0.889TrpSer: 0.889 ± 0.019	0.799TrpThr: 0.799 ± 0.019	0.768TrpVal: 0.768 ± 0.016	0.22TrpTrp: 0.22 ± 0.01	0.302TrpTyr: 0.302 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.589TyrAla: 2.589 ± 0.032	0.248TyrCys: 0.248 ± 0.01	1.322TyrAsp: 1.322 ± 0.023	1.178TyrGlu: 1.178 ± 0.022	0.887TyrPhe: 0.887 ± 0.019	2.083TyrGly: 2.083 ± 0.031	0.458TyrHis: 0.458 ± 0.014	0.76TyrIle: 0.76 ± 0.017	0.549TyrLys: 0.549 ± 0.013	2.321TyrLeu: 2.321 ± 0.028	0.389TyrMet: 0.389 ± 0.011	0.52TyrAsn: 0.52 ± 0.016	1.083TyrPro: 1.083 ± 0.02	0.695TyrGln: 0.695 ± 0.017	1.797TyrArg: 1.797 ± 0.028	1.094TyrSer: 1.094 ± 0.021	1.024TyrThr: 1.024 ± 0.022	1.676TyrVal: 1.676 ± 0.027	0.4TyrTrp: 0.4 ± 0.011	0.567TyrTyr: 0.567 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9038 proteins (2831079 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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