Mycobacterium phage Kingsley

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 112 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JTS0|A0A1W6JTS0_9CAUD Uncharacterized protein OS=Mycobacterium phage Kingsley OX=1970794 GN=SEA_KINGSLEY_58 PE=4 SV=1
MM1 pKa = 7.19NASEE5 pKa = 5.76DD6 pKa = 3.69GLEE9 pKa = 4.36PLGEE13 pKa = 4.45APDD16 pKa = 4.44LGAPHH21 pKa = 6.8YY22 pKa = 10.26PMSAEE27 pKa = 4.24VTFHH31 pKa = 7.62RR32 pKa = 11.84ARR34 pKa = 11.84CTKK37 pKa = 10.5CGDD40 pKa = 3.08IEE42 pKa = 4.31TDD44 pKa = 3.37YY45 pKa = 11.82GDD47 pKa = 4.16FSAYY51 pKa = 10.05GDD53 pKa = 3.5PGGAINAVLAAADD66 pKa = 3.49WFGRR70 pKa = 11.84SAPTGEE76 pKa = 5.08LIDD79 pKa = 4.08FGGGRR84 pKa = 11.84MGQRR88 pKa = 11.84YY89 pKa = 7.81QLVEE93 pKa = 4.46LLCPDD98 pKa = 3.92CQRR101 pKa = 11.84CEE103 pKa = 3.95VCGTAKK109 pKa = 10.44AYY111 pKa = 9.85PIDD114 pKa = 3.85DD115 pKa = 4.0HH116 pKa = 6.93LVCEE120 pKa = 4.29DD121 pKa = 4.12HH122 pKa = 7.29EE123 pKa = 4.31DD124 pKa = 3.94HH125 pKa = 6.79EE126 pKa = 5.25FEE128 pKa = 4.96AAPP131 pKa = 3.95

Molecular weight: 14.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JTP9|A0A1W6JTP9_9CAUD Uncharacterized protein OS=Mycobacterium phage Kingsley OX=1970794 GN=SEA_KINGSLEY_61 PE=4 SV=1
MM1 pKa = 7.57RR2 pKa = 11.84RR3 pKa = 11.84GAKK6 pKa = 9.71LPEE9 pKa = 4.09WVVEE13 pKa = 4.49RR14 pKa = 11.84IVALSWNGWTTGDD27 pKa = 3.08IAKK30 pKa = 10.25EE31 pKa = 4.2VGCSDD36 pKa = 3.33RR37 pKa = 11.84TVSRR41 pKa = 11.84VRR43 pKa = 11.84VRR45 pKa = 11.84HH46 pKa = 5.55GISRR50 pKa = 11.84GEE52 pKa = 3.86AHH54 pKa = 7.28DD55 pKa = 5.3PIPEE59 pKa = 4.15HH60 pKa = 6.98VLAQAARR67 pKa = 11.84LLDD70 pKa = 4.61DD71 pKa = 4.18GASYY75 pKa = 10.87TDD77 pKa = 3.35AAATVGCSRR86 pKa = 11.84TALRR90 pKa = 11.84RR91 pKa = 11.84KK92 pKa = 9.78FPGRR96 pKa = 11.84GWDD99 pKa = 3.23RR100 pKa = 11.84RR101 pKa = 11.84QCAEE105 pKa = 3.42WRR107 pKa = 11.84VIARR111 pKa = 11.84MEE113 pKa = 3.81RR114 pKa = 11.84AIAA117 pKa = 3.76

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

112

0

112

19469

25

1176

173.8

19.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.499 ± 0.384

1.325 ± 0.179

6.657 ± 0.22

5.861 ± 0.339

2.892 ± 0.153

8.675 ± 0.575

2.209 ± 0.168

4.474 ± 0.149

3.508 ± 0.194

7.273 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.286 ± 0.129

3.231 ± 0.2

6.087 ± 0.206

3.518 ± 0.186

7.047 ± 0.409

5.891 ± 0.218

6.672 ± 0.332

7.211 ± 0.279

2.101 ± 0.11

2.584 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski