Aspergillus foetidus slow virus 1
Average proteome isoelectric point is 7.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J3JSF7|J3JSF7_9VIRU Coat protein OS=Aspergillus foetidus slow virus 1 OX=1087070 GN=CP PE=4 SV=1
MM1 pKa = 6.83 STIVRR6 pKa = 11.84 NGYY9 pKa = 8.85 LASVLANPRR18 pKa = 11.84 GTRR21 pKa = 11.84 INGDD25 pKa = 3.26 AQFRR29 pKa = 11.84 RR30 pKa = 11.84 YY31 pKa = 9.05 RR32 pKa = 11.84 ANIRR36 pKa = 11.84 TEE38 pKa = 3.88 ATVGGVNDD46 pKa = 3.58 ARR48 pKa = 11.84 HH49 pKa = 5.2 VQVSYY54 pKa = 10.85 EE55 pKa = 3.88 VGRR58 pKa = 11.84 VHH60 pKa = 6.13 RR61 pKa = 11.84 TKK63 pKa = 10.88 GAALAAAADD72 pKa = 3.56 NLVRR76 pKa = 11.84 VEE78 pKa = 4.04 AAYY81 pKa = 7.63 PTADD85 pKa = 3.23 TLVEE89 pKa = 4.22 DD90 pKa = 5.84 FIGLAKK96 pKa = 10.29 KK97 pKa = 10.42 YY98 pKa = 10.34 SNFSASFEE106 pKa = 4.18 YY107 pKa = 10.81 SSLAGVAEE115 pKa = 4.24 RR116 pKa = 11.84 LAKK119 pKa = 10.38 GLAASSIWDD128 pKa = 4.02 DD129 pKa = 3.43 MTATDD134 pKa = 4.17 LRR136 pKa = 11.84 GNRR139 pKa = 11.84 VLNISVLGTHH149 pKa = 7.29 DD150 pKa = 4.04 GPVNSLTNAVFIPRR164 pKa = 11.84 LVNTAVTGDD173 pKa = 3.7 VFTVMINAVAGEE185 pKa = 4.48 GSAVVTDD192 pKa = 3.89 VLEE195 pKa = 4.36 LDD197 pKa = 3.52 AATRR201 pKa = 11.84 QPIVATVDD209 pKa = 2.95 ADD211 pKa = 3.83 GFSRR215 pKa = 11.84 ACVEE219 pKa = 3.8 ALRR222 pKa = 11.84 IIGANMLAADD232 pKa = 4.09 QGPLFAYY239 pKa = 9.8 AVTRR243 pKa = 11.84 GIHH246 pKa = 5.5 NVFTVVGHH254 pKa = 5.33 TDD256 pKa = 2.98 EE257 pKa = 5.75 GGVSRR262 pKa = 11.84 DD263 pKa = 3.01 WLRR266 pKa = 11.84 ASGFAPPFGGIHH278 pKa = 6.27 YY279 pKa = 7.88 GLPVYY284 pKa = 10.65 SGLPALSSTAPADD297 pKa = 3.16 VSAYY301 pKa = 9.77 VDD303 pKa = 4.76 CIALTSAALVAHH315 pKa = 7.14 SDD317 pKa = 3.39 PGITYY322 pKa = 10.0 DD323 pKa = 4.59 DD324 pKa = 2.93 RR325 pKa = 11.84 WYY327 pKa = 10.14 PSFYY331 pKa = 10.25 QGTGADD337 pKa = 3.6 TAEE340 pKa = 4.16 VRR342 pKa = 11.84 PGQHH346 pKa = 5.95 QDD348 pKa = 2.59 GTADD352 pKa = 3.07 MGRR355 pKa = 11.84 RR356 pKa = 11.84 NWGQYY361 pKa = 6.63 TSNMPRR367 pKa = 11.84 FLDD370 pKa = 3.78 YY371 pKa = 11.09 YY372 pKa = 11.19 LPGLGKK378 pKa = 10.7 LFGADD383 pKa = 2.97 GSVQLACTVARR394 pKa = 11.84 THH396 pKa = 6.41 SGLMPISRR404 pKa = 11.84 HH405 pKa = 4.85 FNYY408 pKa = 10.61 ASIAPFFWIEE418 pKa = 3.85 PTSLIPHH425 pKa = 7.28 DD426 pKa = 4.36 FLGSPAEE433 pKa = 4.14 LNGSGSYY440 pKa = 9.09 CTVDD444 pKa = 3.5 VPRR447 pKa = 11.84 EE448 pKa = 3.7 KK449 pKa = 10.88 AGFEE453 pKa = 5.03 DD454 pKa = 3.5 ITAAGAADD462 pKa = 3.81 VAYY465 pKa = 10.29 SAYY468 pKa = 10.04 NVLMRR473 pKa = 11.84 NPRR476 pKa = 11.84 PAWFFAHH483 pKa = 7.21 WLNHH487 pKa = 5.67 PLNGLGAISVRR498 pKa = 11.84 QLDD501 pKa = 4.14 PNGIIHH507 pKa = 7.41 PGACPDD513 pKa = 3.74 HH514 pKa = 7.23 PDD516 pKa = 2.97 VRR518 pKa = 11.84 DD519 pKa = 3.4 RR520 pKa = 11.84 VEE522 pKa = 5.07 AALPWTSFMWTRR534 pKa = 11.84 GQSPFPAPGEE544 pKa = 3.86 LLNLSGTAGFMVKK557 pKa = 10.08 HH558 pKa = 5.67 LTFDD562 pKa = 4.23 DD563 pKa = 4.32 DD564 pKa = 5.61 AIPTPEE570 pKa = 4.37 HH571 pKa = 6.7 LPTARR576 pKa = 11.84 EE577 pKa = 3.9 FEE579 pKa = 4.44 GVSVRR584 pKa = 11.84 ITCGRR589 pKa = 11.84 PMSIANGTSNSPDD602 pKa = 2.9 STARR606 pKa = 11.84 RR607 pKa = 11.84 ARR609 pKa = 11.84 TRR611 pKa = 11.84 AARR614 pKa = 11.84 EE615 pKa = 3.65 LAAATARR622 pKa = 11.84 DD623 pKa = 3.7 RR624 pKa = 11.84 LFGRR628 pKa = 11.84 PAVAEE633 pKa = 4.1 MPILTTAPVMRR644 pKa = 11.84 TKK646 pKa = 10.6 VAAPPPQEE654 pKa = 4.23 PPQVSGGVSGWSRR667 pKa = 11.84 AVPAAEE673 pKa = 4.36 NGVNDD678 pKa = 4.75 DD679 pKa = 4.51 RR680 pKa = 11.84 NPDD683 pKa = 3.34 GAAVPVVPHH692 pKa = 5.66 YY693 pKa = 10.95 NSXKK697 pKa = 9.7 FPVVARR703 pKa = 11.84 PQAGAVIGGGAAPAAPGAPAADD725 pKa = 4.28 EE726 pKa = 5.59 DD727 pKa = 5.37 DD728 pKa = 4.22 NQPPQPVAIGNXNN741 pKa = 3.33
Molecular weight: 78.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.639
IPC2_protein 5.525
IPC_protein 5.575
Toseland 6.02
ProMoST 5.919
Dawson 5.83
Bjellqvist 5.842
Wikipedia 5.83
Rodwell 5.804
Grimsley 6.198
Solomon 5.83
Lehninger 5.817
Nozaki 6.084
DTASelect 6.287
Thurlkill 6.275
EMBOSS 6.237
Sillero 6.186
Patrickios 4.126
IPC_peptide 5.842
IPC2_peptide 6.198
IPC2.peptide.svr19 6.107
Protein with the highest isoelectric point:
>tr|J3JSF7|J3JSF7_9VIRU Coat protein OS=Aspergillus foetidus slow virus 1 OX=1087070 GN=CP PE=4 SV=1
MM1 pKa = 8.25 DD2 pKa = 3.63 IQTRR6 pKa = 11.84 ISEE9 pKa = 4.48 FGRR12 pKa = 11.84 IGTYY16 pKa = 10.6 LFGLINGTDD25 pKa = 3.89 FMDD28 pKa = 4.05 QINSHH33 pKa = 5.07 GTYY36 pKa = 8.99 VAKK39 pKa = 10.92 LIALRR44 pKa = 11.84 NSMSSLRR51 pKa = 11.84 EE52 pKa = 3.65 RR53 pKa = 11.84 HH54 pKa = 5.89 ALLPAAASLLLLPFPLQVEE73 pKa = 4.12 LSIGDD78 pKa = 3.6 VLLLLRR84 pKa = 11.84 SAFNLSYY91 pKa = 9.96 GTTIQAAISSNAIPEE106 pKa = 4.12 ILSDD110 pKa = 3.77 NLAGRR115 pKa = 11.84 ARR117 pKa = 11.84 RR118 pKa = 11.84 IVTLVTQRR126 pKa = 11.84 PDD128 pKa = 2.98 FRR130 pKa = 11.84 EE131 pKa = 3.84 KK132 pKa = 9.96 YY133 pKa = 10.25 FPVKK137 pKa = 10.03 KK138 pKa = 10.21 HH139 pKa = 6.32 PGAQTKK145 pKa = 10.94 ANVHH149 pKa = 5.6 LAGLVRR155 pKa = 11.84 SAVVLHH161 pKa = 5.87 GRR163 pKa = 11.84 GIIGRR168 pKa = 11.84 VLLATAGRR176 pKa = 11.84 LTDD179 pKa = 4.13 DD180 pKa = 3.6 QASSLLIYY188 pKa = 10.69 GSGLLPTFGALGWAIATHH206 pKa = 5.4 MVLYY210 pKa = 10.29 PDD212 pKa = 4.99 DD213 pKa = 4.9 AKK215 pKa = 11.51 ALNGALKK222 pKa = 10.76 ALGTNSTPYY231 pKa = 10.81 GCLLVEE237 pKa = 4.61 ANTLQGRR244 pKa = 11.84 GVGAIDD250 pKa = 3.4 MRR252 pKa = 11.84 EE253 pKa = 3.98 KK254 pKa = 10.78 ARR256 pKa = 11.84 EE257 pKa = 3.76 RR258 pKa = 11.84 CDD260 pKa = 3.31 PDD262 pKa = 5.56 FIQKK266 pKa = 10.19 SVVSANFDD274 pKa = 3.47 EE275 pKa = 4.89 LRR277 pKa = 11.84 EE278 pKa = 4.0 HH279 pKa = 5.89 VRR281 pKa = 11.84 AVITTEE287 pKa = 3.65 LSGRR291 pKa = 11.84 DD292 pKa = 3.28 VKK294 pKa = 10.9 MPTLSDD300 pKa = 3.59 FWTSRR305 pKa = 11.84 WAWCVNGSEE314 pKa = 4.34 TAKK317 pKa = 10.46 SDD319 pKa = 3.56 EE320 pKa = 4.43 VLGLDD325 pKa = 3.38 PKK327 pKa = 10.76 AYY329 pKa = 9.77 KK330 pKa = 9.16 RR331 pKa = 11.84 THH333 pKa = 4.52 TRR335 pKa = 11.84 SYY337 pKa = 10.77 RR338 pKa = 11.84 RR339 pKa = 11.84 MAAEE343 pKa = 4.07 SVRR346 pKa = 11.84 DD347 pKa = 3.88 EE348 pKa = 5.72 PISTWDD354 pKa = 3.11 GTTYY358 pKa = 11.35 ASASPKK364 pKa = 10.55 LEE366 pKa = 4.16 AGKK369 pKa = 8.26 TRR371 pKa = 11.84 AIFACDD377 pKa = 3.1 TRR379 pKa = 11.84 SYY381 pKa = 11.04 FAFSWCLNAVQKK393 pKa = 10.68 AWRR396 pKa = 11.84 NSRR399 pKa = 11.84 VLLDD403 pKa = 3.95 PGIGGHH409 pKa = 6.59 LGMVNRR415 pKa = 11.84 IRR417 pKa = 11.84 HH418 pKa = 4.75 AQRR421 pKa = 11.84 GGGVNLMLDD430 pKa = 3.56 FDD432 pKa = 5.04 DD433 pKa = 6.45 FNSQHH438 pKa = 6.08 HH439 pKa = 5.44 TVAMQIVFDD448 pKa = 4.56 EE449 pKa = 4.34 LCKK452 pKa = 11.12 YY453 pKa = 10.23 IGAPDD458 pKa = 3.97 WYY460 pKa = 10.52 RR461 pKa = 11.84 HH462 pKa = 4.54 VLVNSFTRR470 pKa = 11.84 THH472 pKa = 5.77 VSIDD476 pKa = 3.51 GRR478 pKa = 11.84 WEE480 pKa = 3.99 LVQGTLMSGHH490 pKa = 7.17 RR491 pKa = 11.84 GTTFINSVLNAAYY504 pKa = 9.68 IRR506 pKa = 11.84 MAVGGPYY513 pKa = 9.75 FDD515 pKa = 5.12 SLLSLHH521 pKa = 6.57 TGDD524 pKa = 5.19 DD525 pKa = 3.71 VYY527 pKa = 11.3 IRR529 pKa = 11.84 ANTLGDD535 pKa = 3.52 CDD537 pKa = 4.69 YY538 pKa = 11.25 ILNRR542 pKa = 11.84 CRR544 pKa = 11.84 DD545 pKa = 3.67 YY546 pKa = 11.61 GCRR549 pKa = 11.84 LNPAKK554 pKa = 10.49 QSVGYY559 pKa = 10.18 YY560 pKa = 8.92 GAEE563 pKa = 4.13 FLRR566 pKa = 11.84 VAIRR570 pKa = 11.84 GEE572 pKa = 3.64 RR573 pKa = 11.84 AYY575 pKa = 10.46 GYY577 pKa = 8.7 FARR580 pKa = 11.84 GVSGFVNGNWTSSDD594 pKa = 3.71 PLSLTEE600 pKa = 4.57 GLSSAIASCRR610 pKa = 11.84 TLINRR615 pKa = 11.84 SGDD618 pKa = 3.22 ASLADD623 pKa = 4.08 LLGPAIRR630 pKa = 11.84 YY631 pKa = 8.51 RR632 pKa = 11.84 RR633 pKa = 11.84 GLTTRR638 pKa = 11.84 QTIQLLRR645 pKa = 11.84 GTLSLEE651 pKa = 3.89 GSPVFNTNYY660 pKa = 9.84 IIRR663 pKa = 11.84 NLRR666 pKa = 11.84 VEE668 pKa = 4.62 GVDD671 pKa = 3.84 KK672 pKa = 10.34 EE673 pKa = 4.62 TVPIDD678 pKa = 3.89 NRR680 pKa = 11.84 WKK682 pKa = 10.38 RR683 pKa = 11.84 YY684 pKa = 7.36 ATTDD688 pKa = 3.39 YY689 pKa = 10.47 LTDD692 pKa = 3.47 HH693 pKa = 7.24 LSPVEE698 pKa = 3.95 VEE700 pKa = 4.8 AIHH703 pKa = 6.76 LAKK706 pKa = 9.91 TDD708 pKa = 4.27 PGPLMIASGYY718 pKa = 10.34 RR719 pKa = 11.84 KK720 pKa = 10.07 GLNLDD725 pKa = 4.0 DD726 pKa = 5.57 KK727 pKa = 10.22 GTPSQVRR734 pKa = 11.84 FRR736 pKa = 11.84 PLPPRR741 pKa = 11.84 LATGFASASQLTARR755 pKa = 11.84 RR756 pKa = 11.84 AEE758 pKa = 4.19 PGCLAKK764 pKa = 10.99 YY765 pKa = 9.5 PVLSLIAGRR774 pKa = 11.84 LTDD777 pKa = 4.18 DD778 pKa = 3.77 EE779 pKa = 4.82 LRR781 pKa = 11.84 HH782 pKa = 5.24 LVAMEE787 pKa = 4.56 GGNNTTSDD795 pKa = 2.93 IRR797 pKa = 11.84 KK798 pKa = 8.37 EE799 pKa = 4.06 AFGEE803 pKa = 4.41 SPTSKK808 pKa = 10.7 NIIGFLSYY816 pKa = 11.38 ADD818 pKa = 3.72 AAALSKK824 pKa = 9.77 VTSSGNIFTSISVRR838 pKa = 11.84 VV839 pKa = 3.49
Molecular weight: 92.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.693
IPC2_protein 8.609
IPC_protein 8.565
Toseland 8.902
ProMoST 8.975
Dawson 9.326
Bjellqvist 9.268
Wikipedia 9.531
Rodwell 9.399
Grimsley 9.37
Solomon 9.37
Lehninger 9.326
Nozaki 9.297
DTASelect 9.151
Thurlkill 9.194
EMBOSS 9.428
Sillero 9.385
Patrickios 4.596
IPC_peptide 9.355
IPC2_peptide 8.17
IPC2.peptide.svr19 7.777
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1580
741
839
790.0
85.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.582 ± 1.673
1.076 ± 0.172
5.949 ± 0.167
3.544 ± 0.11
3.608 ± 0.111
8.861 ± 0.204
2.342 ± 0.056
4.747 ± 0.539
2.278 ± 0.688
8.924 ± 1.582
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.709 ± 0.029
4.367 ± 0.143
5.759 ± 1.337
2.215 ± 0.036
7.532 ± 0.507
7.089 ± 0.569
6.582 ± 0.242
7.215 ± 0.832
1.329 ± 0.013
3.165 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here