Physeter macrocephalus (Sperm whale) (Physeter catodon)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Artiodactyla;

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41868 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A455B9Y6|A0A455B9Y6_PHYMC RNA-binding protein EWS OS=Physeter macrocephalus OX=9755 GN=LOC102983969 PE=3 SV=1
MM1 pKa = 7.97DD2 pKa = 3.9VLFVAILAVPLILGQEE18 pKa = 4.28YY19 pKa = 10.35EE20 pKa = 4.25DD21 pKa = 4.2EE22 pKa = 4.59EE23 pKa = 4.67GLEE26 pKa = 4.09EE27 pKa = 4.86DD28 pKa = 5.18DD29 pKa = 4.61YY30 pKa = 11.18YY31 pKa = 11.46QVVYY35 pKa = 10.49YY36 pKa = 7.58YY37 pKa = 10.36TVTPNYY43 pKa = 10.56DD44 pKa = 3.23DD45 pKa = 4.83FGANFTVDD53 pKa = 3.11YY54 pKa = 11.65SMFEE58 pKa = 3.98SEE60 pKa = 4.61NRR62 pKa = 11.84LNKK65 pKa = 9.79LDD67 pKa = 4.07KK68 pKa = 10.75EE69 pKa = 4.28VMEE72 pKa = 4.44AVEE75 pKa = 4.46TTVSRR80 pKa = 11.84EE81 pKa = 3.62TDD83 pKa = 3.34PADD86 pKa = 3.27HH87 pKa = 6.24QKK89 pKa = 10.63PVTEE93 pKa = 4.83KK94 pKa = 9.48PVKK97 pKa = 9.79MEE99 pKa = 4.14PQSPDD104 pKa = 2.94LNNAVSSLQSPGVLLLSWALVQGGMYY130 pKa = 10.45FMM132 pKa = 6.15

Molecular weight: 15.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A455BHE3|A0A455BHE3_PHYMC threonine--tRNA ligase mitochondrial 1-like OS=Physeter macrocephalus OX=9755 GN=LOC114485773 PE=4 SV=1
MM1 pKa = 7.03QWCQRR6 pKa = 11.84SSPGGVGGGGRR17 pKa = 11.84RR18 pKa = 11.84GRR20 pKa = 11.84GLARR24 pKa = 11.84VRR26 pKa = 11.84RR27 pKa = 11.84ARR29 pKa = 11.84LGSAGARR36 pKa = 11.84AALQLGPRR44 pKa = 11.84LRR46 pKa = 11.84AAPLLAPLWLLAPTPGSHH64 pKa = 5.57MTPAPLALRR73 pKa = 11.84ASQGWRR79 pKa = 11.84GKK81 pKa = 10.48FLLLVV86 pKa = 3.86

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20083

21785

41868

27548336

31

35134

658.0

73.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.124 ± 0.012

2.127 ± 0.01

4.862 ± 0.009

7.277 ± 0.017

3.47 ± 0.008

6.493 ± 0.016

2.592 ± 0.006

4.17 ± 0.01

5.787 ± 0.015

9.828 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.086 ± 0.005

3.519 ± 0.009

6.482 ± 0.019

4.913 ± 0.014

5.837 ± 0.012

8.526 ± 0.014

5.232 ± 0.009

5.96 ± 0.01

1.159 ± 0.004

2.543 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski